Depression of Macrophages Modifies Serum Lipid Profile in Hyperlipidemia

Abstract

Suppression of functional activity of macrophages by gadolinium chloride, suppressing the macrophage population and the endocytosis velocity, was studied in vivo. Injection of GdCl3 led to an increase in serum cholesterol concentration. Preliminary injection of GdCl3 to mice with lipidemia 24 h before poloxamer 407 reduced the concentrations of triglycerides and LDL during marked depression of macrophages (in 24 h). Macrophage repopulation (days 5, 7) was associated with development of a trend to an increase of triglyceride and LDL levels. lectron microscopic study of Kupffer cells after injection of poloxamer 407 and its combination with gadoliniun chloride detected the intralysosomal accumulation syndrome in these cells (formation of auto- and heterophagolysosomes). Activity of cathepsin B, characteristic of macrophages, reduced 24 h after injections of GdCl3 and poloxamer 407 alone and restored in response to their combination.

Treatment with Autophagy Inducer Trehalose Alleviates Memory and Behavioral Impairments and Neuroinflammatory Brain Processes in db/db Mice

“…Mice were subdivided into four groups (6-8 animals each): (1) WT mice drinking water ad libitum during the whole experiment (24 days); (2) WT mice drinking 2% trehalose solution instead; (3) db/db mice drinking water ad libitum during the whole experiment; (4) db/db mice drinking 2% trehalose solution instead. Behavior testing began two weeks after the start of the experiment (on days [15][16][17][18][19][20][21][22][23][24] without stopping the consumption of trehalose. On the day of euthanasia (day 25), animals of all groups were culled with CO 2 and transcardially perfused as described below (Section 2.5).…”

“…The blood samples were collected during chest dissection prior to transcardial perfusion. The blood collection, serum preparation and storage, and biochemical assays in the serum were performed as described earlier [22]. Total serum cholesterol and triglycerides (TGs) were quantified on an Architect c8000 biochemical analyzer (Abbott, Abbott Park, IL, USA), whereas low-density lipoprotein (LDL) cholesterol was assayed with the Biosystems kits, Costa Brava (Biosystems S.A., Barcelona, Spain) on a Labio 200 biochemical analyzer BS-200 (Shenzhen Mindray Bio-Medical Electronics Co., Ltd., Shenzhen, China) [23,24].…”