Recombinant canarypox virus expressing the VP2 protein of infectious bursal disease virus induces protection in vaccinated SPF chickens

Zanetti, Flavia; Cardona, Romina; Federico, Carlos Rodolfo; Chimeno-Zoth, Silvina; Calamante, Gabriela

doi:10.1007/s12250-015-3680-6

Cited by 5 publications

(4 citation statements)

References 12 publications

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“…However, an interesting finding of this study is that, despite not being significantly different, the histopathological scores were slightly better in the group primed with pVP2 and boosted with rVP2. This contrasts with the results of the humoral evaluation, although is consistent with other reports that found that less or even no detectable antibodies did not obliterate the ability of certain vaccine candidates to protect in both avian [11,36,37] and mammalian [38,39] models.…”

Section: Discussionsupporting

confidence: 88%

“…In order to overcome these issues, different immunogens such as subunit, DNA or live viral-vectored vaccines have been rationally designed and evaluated [6,7]. Our group has had successful experiences immunizing chickens with VP2 protein produced both in Nicotiana benthamiana plants [8] and through non replicative poxviral vectors [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

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Comparison of homologous and heterologous prime-boost immunizations combining MVA-vectored and plant-derived VP2 as a strategy against IBDV

Richetta

Gómez

Lucero³

et al. 2017

Vaccine

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Different immunogens such as subunit, DNA or live viral-vectored vaccines against Infectious Bursal Disease virus (IBDV) have been evaluated in the last years. However, the heterologous prime-boost approach using recombinant modified vaccinia Ankara virus (rMVA), which has shown promising results in both mammals and chickens, has not been tried against this pathogen yet. IBD is a highly contagious and immunosuppressive disease of poultry that affects mainly young chicks. It is caused by IBDV, a double-stranded RNA virus carrying its main antigenic epitopes on the capsid protein VP2. Our objective was to evaluate the immune response elicited by two heterologous prime-boost schemes combining an rMVA carrying the VP2 mature gene (rVP2) and a recombinant VP2 protein produced in Nicotiana benthamiana (pVP2), and to compare them with the performance of the homologous pVP2-pVP2 scheme usually used in our laboratory. The SPF chickens immunized with the three evaluated schemes elicited significantly higher anti-VP2 antibody titers (p<0.001) and seroneutralizing titers (p<0.05) and had less T-cell infiltration (p<0.001), histological damage (p<0.001) and IBDV particles (p<0.001) in their bursae of Fabricius when compared with control groups. No significant differences were found between both heterologous schemes and the homologous one. However, the rVP2-pVP2 scheme showed significantly higher anti-VP2 antibody titers than pVP2-rVP2 and a similar tendency was found in the seroneutralization assay. Conversely, pVP2-rVP2 had the best performance when evaluated through bursal parameters despite having a less potent humoral immune response. These findings suggest that the order in which rVP2 and pVP2 are combined can influence the immune response obtained. Besides, the lack of a strong humoral immune response did not lessen the ability to protect from IBDV challenge. Therefore, further research is needed to evaluate the mechanisms by which these immunogens are working in order to define the combination that performs better against IBDV.

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Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Comparison of homologous and heterologous prime-boost immunizations combining MVA-vectored and plant-derived VP2 as a strategy against IBDV

Richetta

Gómez

Lucero³

et al. 2017

Vaccine

Self Cite

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“…Despite the fact that not all chicken had specific anti-VP2 antibodies and that VN titers might not be as high as the ones observed when the immunogen is im administered, we were able to see protection against viral infection evidenced by BB ratio, T-cell infiltration, and viral load in bursa, in all but one animal. Although neutralizing antibodies have been considered as the most relevant tool to protect against IBDV infection, there is evidence that protection can be achieved in their absence, suggesting that other mechanisms might also be relevant in the defense against IBDV ( Yeh et al, 2002 ; Ingrao et al, 2013 ; Zanetti et al, 2016 ). On the other hand, in this experiment, we did not measure mucosal IgA in the intestine, which is one of the desirable immune responses pursued by oral vaccines.…”

Section: Discussionmentioning

confidence: 99%

Oral Immunization With Plant-Based Vaccine Induces a Protective Response Against Infectious Bursal Disease

et al. 2021

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Infectious bursal disease virus (IBDV) is the etiological agent of an immunosuppressive and highly contagious disease that affects young birds causing important economic losses in the poultry industry worldwide. We have previously developed a plant-based vaccine candidate for infectious bursal disease (IBD) that is able to protect against infection with IBDV when administered through intramuscular (im) route. Given that oral vaccination is non-invasive and stimulates the immunity of the mucosal gastrointestinal surface, the initial site of contact and entry of IBDV, the aim of this work was to study if our immunogen was also able to elicit a protective immune response when orally administered. We demonstrated that 85% of the animals that received two oral doses of the vaccine formulation and all animals that were orally boosted after an im prime scheme developed virus neutralizing antibodies and were protected against IBDV infection, evidenced by the bursa/body weight (BB) ratio, absence of T-cell infiltration, and low viral load in bursa. Although mild to moderate bursal damage was observed in some of these animals, these lesions were not as severe as the ones observed in challenged control groups, which also presented signs of acute inflammation, bursal atrophy, T-cell infiltration, and absence of viral clearance. These results show that two immunizations with our recombinant immunogen are able to induce a specific and protective immune response in chicken against IBDV when orally administered in a prime/boost scheme or when the oral boost follows an im prime scheme. In conclusion, our oral plant-based vaccine candidate could represent a viable alternative to conventional vaccines and is of great interest to the poultry industry.

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“…Other viral vector vaccines. A canarypox vector virus has been developed for chickens as a vaccine against IBD in Argentina (188,358,359). This vector is poorly or nonreplicative in chickens and therefore, high dose is required to induce protection (358).…”

Section: New Technology Vaccinesmentioning

confidence: 99%

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Avian Diseases

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Recombinant canarypox virus expressing the VP2 protein of infectious bursal disease virus induces protection in vaccinated SPF chickens

Cited by 5 publications

References 12 publications

Comparison of homologous and heterologous prime-boost immunizations combining MVA-vectored and plant-derived VP2 as a strategy against IBDV

Comparison of homologous and heterologous prime-boost immunizations combining MVA-vectored and plant-derived VP2 as a strategy against IBDV

Oral Immunization With Plant-Based Vaccine Induces a Protective Response Against Infectious Bursal Disease

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