Variation in sequences containing microsatellite motifs in the perennial biomass and forage grass, Phalaris arundinacea (Poaceae)

Barth, Susanne; Jankowska, M.; Hodkinson, Trevor R.; Vellani, Tia; Klaas, Manfred

doi:10.1186/s13104-016-1994-6

Cited by 6 publications

(14 citation statements)

References 15 publications

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“…arundinacea contrasts with analogous data for P . arundinacea populations sampled in different ways and greater distances (Anderson et al, ; Barth et al, ; Jakubowski et al, ; Jakubowski et al, ; Jakubowski, Jackson, et al, ; Nelson et al, ; Perdereau et al, ). In our study, molecular variance within populations was lower (69%) compared with the same parameter (74.5%) obtained for SSR loci of cultivars and wild accessions of P .…”

Section: Discussionmentioning

confidence: 99%

“…In our study, molecular variance within populations was lower (69%) compared with the same parameter (74.5%) obtained for SSR loci of cultivars and wild accessions of P . arundinacea sampled on the global scale (Jakubowski, Jackson, et al, ), 85% obtained for ISSR loci of populations sampled on the global scale (Anderson et al, ), or for others used for SSR loci (Barth et al, ; Perdereau et al, ). This lowered variation in the Merkys populations could be due to close proximity and shared gene flow in this wind‐pollinated species.…”

Section: Discussionmentioning

confidence: 99%

“…An advantage of studying P . arundinacea is the large set of molecular markers applied (Anderson et al, ; Barth, Jankowska, Hodkinson, Vellani, & Klaas, ; Bloju, Boldura, Samfira, & Popescu, ; Casler et al, ; Jakubowski, Casler, & Jackson, ; Jakubowski, Jackson, Johnson, Hu, & Casler, ; Jakubowski, Casler, & Jackson, ; Jakubowski, Jackson, & Casler, ; Lavergne & Molofsky, ; Nelson et al, ; Perdereau, Klaas, Barth, & Hodkinson, ), providing tested methodological approaches for further research.…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity of phalaris arundinacea populations in relation to river regulation in the Merkys basin, Lithuania

Anderson

Jocienė

Krokaitė

et al. 2018

River Research & Apps

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During the 1950s-1960s, the Merkys river basin, the largest protected area of Lithuania, underwent severe anthropogenic regulations. Within the Baltic States, the genetic diversity of Phalaris arundinacea populations is unknown and how they might be affected by anthropogenic activities such as river regulation. The objectives of this study were to compare molecular parameters (SSRs or microsatellite loci) within and among populations from natural river fragments with populations from regulated river parts. Study populations have greater genetic diversity within, rather than among, populations. The upstream portion of the Merkys basin populations had lower genetic diversity compared with further downstream. The mean number of polymorphic SSR loci was lower for populations from regulated parts of the river basin compared with natural ones. Main principle coordinate analysis revealed populations of regulated rivers at marginal positions. Bayesian clustering showed that current populations are admixtures of 3 distinct genetic groups, based on STRUCTURE analysis (K = 3 groupings) in geographic subdivisions of (a) downstream populations (Varėnė, Verseka, Upper Grūda, Lower Grūda, Upper Merkys, Lower Merkys), (b) upstream populations (Upper Šalčia, Beržė, Lower Šalčia, Visinčia, Lower Šaltykščia, Nedilė), and (c) the 2 regulated sites in the distinct geographic area of Taurupis and Upper Šaltykščia.P. arundinacea in the Merkys river basin does not all belong to a single, random-mating population encompassing its tributaries or among populations across its geographic scales. In several instances, river regulation might impair the genetic diversity of P. arundinacea populations.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genetic diversity of phalaris arundinacea populations in relation to river regulation in the Merkys basin, Lithuania

Anderson

Jocienė

Krokaitė

et al. 2018

River Research & Apps

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“…This is a new molecular technology approach for use with reed canarygrass. Previous research used inter-SSRs [ISSRs (Anderson et al, 2016;K avov a et al, 2018)], SSRs (Anderson et al, 2018;Jakubowski et al, 2014), or expressed sequence tag (EST)-SSR markers (Barth et al, 2016). This preliminary research was necessary for trouble-shooting methodology before performing full-scale reed canarygrass population structure analyses to distinguish between native and exotic reed canarygrass in our core collection of samples.…”

Section: Use Of Diversity Arraysmentioning

confidence: 99%

Challenges of Establishing Native versus Exotic Status of Herbarium Specimens

Noyszewski

Anderson

Smith

et al. 2019

hortte

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In cases where invasive species are presumed to be strictly exotic, the discovery that the species is also native can be disconcerting for researchers and land managers responsible for eradicating an exotic invasive. Such is the case with reed canarygrass (Phalaris arundinacea), for which decades of misinformation led to the call for nationwide control of this species in the United States. However, native populations were first reported by LaVoie and then later confirmed by Casler with molecular analyses. This, coupled with the discovery by Anderson that this species has been used in weavings by Native Americans for centuries, also made the native forms of interest for protection. Identifying the native status of historic, herbarium specimens via molecular analyses is of great interest to determine localities of native populations for confirmation with extant specimens. Genetic-based methods describing DNA polymorphism of reed canary grass are not well developed. The goal of the presented research is to assess the utility of genomic DNA obtained from historic (herbaria) and extant (fresh) tissue of reed canarygrass and the application of using Diversity Arrays Technology sequencing low density for genetic population studies.

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“…Slight changes in the genetic code, which include simple sequence repeats (SSRs) and single nucleotide polymorphisms (SNPs), are directly linked to phenotypic differences (Barth et al, 2016). It is important that introgression of SG QTL does not have adverse effects on other aspects of the phenotype.…”

Section: Plant Height and Floweringmentioning

confidence: 99%

A comparative assessment of the performance of a stay-green sorghum (Sorghum bicolor (L) Moench) introgression line developed by marker-assisted selection and its parental lines

Galyuon¹,

Hash²,

Bidinger³

et al. 2019

Afr. J. Biotechnol.

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A glasshouse experiment was conducted to compare the performance of a stay-green QTL introgression line (RSG 03123) and its parental lines (B35, a stay-green and R16, a high yielding cultivar with rapid senescence) after flowering, when water-limited (WL) or well-watered (WW). Flowering time and plant height were similar in RSG 03123 and the recurrent parent, R16. Under WW conditions, retention of green leaf area (GLA) and chlorophyll was similar in RSG 03123 and R16, whereas under WL conditions RSG 03123 retained significantly (P<0.05) more GLA. The rate of senescence was also lower in RSG 03123 as compared to R16 under WL conditions. B35 displayed a delayed onset of senescence under both WW and WL conditions while both R16 and RSG 01323 displayed an earlier onset of senescence under WL conditions. Photochemical efficiency of photosystem II (ΦPSII), gas exchange characteristics, and total soluble carbohydrates contents, in both leaf and stem, were higher in RSG 03123 than in R16 under WL conditions. These parameters were enhanced in RSG 03123 due to higher retention of chloroplast proteins, such as light-harvesting chlorophyll binding protein complex of PSII (LHCPII), oxygen-evolving complex 33 kDa (OEC33), phosphoenolpyruvate carboxylase (PEPC) and the large subunit of ribulose bisphosphate carboxylase-oxygenase (Rubisco). The stay-green characteristics of RSG 03123 were intermediate between R16 and B35; nonetheless, the results show that stay-green QTL are functional during senescence and improve tolerance to water limitation after flowering.

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Variation in sequences containing microsatellite motifs in the perennial biomass and forage grass, Phalaris arundinacea (Poaceae)

Cited by 6 publications

References 15 publications

Genetic diversity of phalaris arundinacea populations in relation to river regulation in the Merkys basin, Lithuania

Genetic diversity of phalaris arundinacea populations in relation to river regulation in the Merkys basin, Lithuania

Challenges of Establishing Native versus Exotic Status of Herbarium Specimens

A comparative assessment of the performance of a stay-green sorghum (Sorghum bicolor (L) Moench) introgression line developed by marker-assisted selection and its parental lines

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