Silk fibroin scaffold as a potential choice for female pelvic reconstruction: A study on the biocompatibility in abdominal wall, pelvic, and vagina

Chang, Yun Woo; Sun, Xiuli; Li, Qi; Ding, Xia; Liu, Haifeng; Wang, Jianliu

doi:10.1002/jemt.22653

Cited by 16 publications

(10 citation statements)

References 29 publications

(35 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Poly(lactic acid) (PLA) dramatically promoted the formation of ECM by increasing the attachment and metabolic activity of fibroblasts on PLA meshes as compared to other meshes: AlloDerm (LifeCell Corp., Branchburg, NJ, USA), cadaveric dermis, porcine dermis, polypropylene, sheep forestomach, and porcine small intestinal submucosa (SIS) [ 84 ]. Silk fibroin (SF) appeared to be successful in promoting the growth of fibroblasts into the scaffold and inducing healthy tissue formation in defective areas such as the abdomen, vagina, and pelvis [ 85 ]. Polylactic-co-glycolic acid/polycaprolactone (PLGA/PCL) electro-spun scaffolds promoted the differentiation and proliferation of myofibroblasts and enhanced the integration of host cells and scaffolds [ 82 ].…”

Section: Role Of Fibroblasts In Wound Healing and Tissue Regenerationmentioning

confidence: 99%

Role of Fibroblasts and Myofibroblasts on the Pathogenesis and Treatment of Pelvic Organ Prolapse

Guler

Roovers

2022

Biomolecules

View full text Add to dashboard Cite

Pelvic organ prolapse (POP) is a multifactorial connective tissue disorder caused by damage to the supportive structures of the pelvic floor, leading to the descent of pelvic organs in the vagina. In women with POP, fibroblast function is disturbed or altered, which causes impaired collagen metabolism that affects the mechanical properties of the tissue. Ideal surgical repair, either native tissue repair or POP surgery using an implant, aims to create a functional pelvic floor that is load-bearing, activating fibroblasts to regulate collagen metabolism without creating fibrotic tissue. Fibroblast function plays a crucial role in the pathophysiology of POP by directly affecting the connective tissue quality. On the other hand, fibroblasts determine the success of the POP treatment, as the fibroblast-to-(myo)fibroblast transition is the key event during wound healing and tissue repair. In this review, we aim to resolve the question of “cause and result” for the fibroblasts in the development and treatment of POP. This review may contribute to preventing the development and progress of anatomical abnormalities involved in POP and to optimizing surgical outcomes.

show abstract

Section: Role Of Fibroblasts In Wound Healing and Tissue Regenerationmentioning

confidence: 99%

Role of Fibroblasts and Myofibroblasts on the Pathogenesis and Treatment of Pelvic Organ Prolapse

Guler

Roovers

2022

Biomolecules

View full text Add to dashboard Cite

show abstract

“…In all these cases, natural fibrin‐agarose hydrogels were combined with stromal adult stem or stromal cells to reproduce the histological structure of native connective tissues by generating a bioengineered stroma. Other researchers described the use of several types of meshes and devices generated by tissue engineering using recellularized dermal acellular matrices and other materials such as silk fibroin, collagen, and intestinal submucosa previously seeded with cells. All these bioengineered substitutes offer high biocompatibility as compared to synthetic materials.…”

Section: Introductionmentioning

confidence: 99%

Generation and Evaluation of Novel Stromal Cell‐Containing Tissue Engineered Artificial Stromas for the Surgical Repair of Abdominal Defects

Martín-Piedra

Garzón

Gómez-Sotelo

et al. 2017

Biotechnology Journal

View full text Add to dashboard Cite

Repair of abdominal wall defects is one of the major clinical challenges in abdominal surgery. Most biomaterials are associated to infection and severe complications, making necessary safer and more biocompatible approaches. In the present work, the adequate mechanical properties of synthetic polymer meshes with tissue-engineered matrices containing stromal mesenchymal cells is combined to generate a novel cell-containing tissue-like artificial stroma (SCTLAS) for use in abdominal wall repair. SCTLAS consisting on fibrin-agarose hydrogels seeded with stromal cells and reinforced with commercial surgical meshes (SM) are evaluated in vitro and in vivo in animal models of abdominal wall defect. Inflammatory cells, collagen, and extracellular matrix (ECM) components are analyzed and compared with grafted SM. Use of SCTLAS results in less inflammation and less fibrosis than SM, with most ECM components being very similar to control abdominal wall tissues. Cell migration and ECM remodeling within SCTLAS is comparable to control tissues. The use of SCTLAS could contribute to reduce the side-effects associated to currently available SM and regenerated tissues are more similar to control abdominal wall tissues. Bioengineered SCTLAS could contribute to a safer treatment of abdominal wall defects with higher biocompatibility than currently available SM.

show abstract

“…There was considerable inflammatory cell infiltration when used as a mesh in the vesicouterine space, although the acute inflammation lasted no more than 4 weeks. 101 …”

Section: The Safety Of Silkmentioning

confidence: 99%

Silk fibroin safety in the eye: a review that highlights a concern

2020

View full text Add to dashboard Cite

The biomedical use of silk as a suture dates back to antiquity. Fibroin is the structural element that determines the strength of silk and here we consider the safety of fibroin in its role in ophthalmology. The high mechanical strength of silk meant sufficiently thin threads could be made for eye microsurgery, but such usage was all but superseded by synthetic polymer sutures, primarily because silk in its entirety was more inflammatory. Significant immunological response can normally be avoided by careful manufacturing to provide high purity fibroin, and it has been utilised in this form for tissue engineering an array of fibre and film substrata deployed in research with cells of the eye. Films of fibroin can also be made transparent, which is a required property in the visual pathway. Transparent layers of corneal epithelial, stromal and endothelial cells have all been demonstrated with maintenance of phenotype, as have constructs supporting retinal cells. Fibroin has a lack of demonstrable infectious agent transfer, an ability to be sterilised and prepared with minimal contamination, long-term predictable degradation and low direct cytotoxicity. However, there remains a known ability to be involved in amyloid formation and potential amyloidosis which, without further examination, is enough to currently question whether fibroin should be employed in the eye given its innervation into the brain.

show abstract

Silk fibroin scaffold as a potential choice for female pelvic reconstruction: A study on the biocompatibility in abdominal wall, pelvic, and vagina

Cited by 16 publications

References 29 publications

Role of Fibroblasts and Myofibroblasts on the Pathogenesis and Treatment of Pelvic Organ Prolapse

Role of Fibroblasts and Myofibroblasts on the Pathogenesis and Treatment of Pelvic Organ Prolapse

Generation and Evaluation of Novel Stromal Cell‐Containing Tissue Engineered Artificial Stromas for the Surgical Repair of Abdominal Defects

Silk fibroin safety in the eye: a review that highlights a concern

Contact Info

Product

Resources

About