Dietary choline regulates antibacterial activity, inflammatory response and barrier function in the gills of grass carp (Ctenopharyngodon idella)

Zhao, Hua-Fu; Jiang, Wei‐Dan; Liu, Yang; Jiang, Jun; Wu, Pei; Kuang, Sheng‐Yao; Tang, Ling; Tang, Wu‐Neng; Zhang, Yongan; Zhou, Xiao‐Qiu; Feng, Lin

doi:10.1016/j.fsi.2016.03.029

Cited by 35 publications

(18 citation statements)

References 72 publications

(125 reference statements)

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“…Both choline and betaine, as the precursor substances of trimethylamine N‐oxide (TMAO), are two essential and nutritional biomolecules. Several reports demonstrated that the deficiency of choline and betaine could accelerate the production of reactive oxygen species (ROS) and are thus considered as a key factor in initiating and maintaining inflammatory diseases caused by oxidative damage [35,36]. Recent studies indicated that the absence of the essential amino acid L‐tryptophan could affect ROS scavenging and immune response control in vitiligo, which is generally regarded as an autoimmune disease and which is also a prominent feature in VKH disease [5,37].…”

Section: Discussionmentioning

confidence: 99%

Integrated omics analysis of sweat reveals an aberrant amino acid metabolism pathway in Vogt–Koyanagi–Harada disease

Cui

Zhang

et al. 2020

Clinical and Experimental Immunology

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Vogt-Koyanagi-Harada (VKH) disease is an autoimmune disease leading to visual impairment. Its pathogenic mechanisms remain poorly understood. Our purpose was to investigate the distinctive protein and metabolic profiles of sweat in patients with VKH disease. In the present study, proteomics and metabolomics analysis was performed on 60 sweat samples (30 VKH patients and 30 normal controls) using liquid chromatography tandem mass spectrometry. Parallel reaction monitoring (PRM) analysis was used to validate the results of our omics analysis. In total, we were able to detect 716 proteins and 175 metabolites. Among them, 116 proteins (99 decreased and 17 increased) were observed to be significantly different in VKH patients when compared to controls. Twenty-one differentially expressed metabolites were identified in VKH patients, of which 18 included choline, L-tryptophan, betaine and L-serine were reduced, while the rest were increased. Our multi-omics strategy reveals an important role for the amino acid metabolic pathway in the pathogenesis of VKH disease. Significant differences in proteins and metabolites were identified in the sweat of VKH patients and, to some extent, an aberrant amino acid metabolism pathway may be a pathogenic factor in the pathogenesis of VKH disease.

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Section: Discussionmentioning

confidence: 99%

Integrated omics analysis of sweat reveals an aberrant amino acid metabolism pathway in Vogt–Koyanagi–Harada disease

Cui

Zhang

et al. 2020

Clinical and Experimental Immunology

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“…Previous studies have demonstrated that choline is a vitamin for young vertebrates, it is the most abundant vitamin constituent in most fish feeds, and it can provide active methyl groups, which can participate in the anabolism of important physiological compounds such as methionine, phospholipids and carnitine (22,24,25,28,29,(44)(45)(46) . Recent studies showed that adequate dietary supplementation of choline could improve growth performance and feed utilisation in various fish species (24,25,(45)(46)(47) .…”

Section: Discussionmentioning

confidence: 99%

“…Recent studies reported that dietary choline supplementation affected hepatic transport and lipid deposition in various fish species, which suggested that dietary choline could reduce hepatic lipid content and influence expression of lipid metabolism genes (23)(24)(25)(26) . Moreover, previous studies demonstrated that dietary choline could modulate immune responses by reducing expression of pro-inflammatory biomarkers such as TNF-α (tnfα), IL-1β (il-1β) and NFκB (nfκb), and up-regulating mRNA expression of anti-inflammatory cytokine IL-10 (il-10) and transforming growth factor β-1 (tgfβ-1) in vertebrates including fish (27)(28)(29) . However, there are few studies investigating the possible mechanisms whereby dietary choline affects lipid metabolism and subsequently reduces inflammatory response.…”

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confidence: 99%

Dietary choline supplementation attenuated high-fat diet-induced inflammation through regulation of lipid metabolism and suppression of NFκB activation in juvenile black seabream (Acanthopagrus schlegelii)

et al. 2019

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The present study aimed to investigate whether dietary choline can regulate lipid metabolism and suppress NFκB activation and, consequently, attenuate inflammation induced by a high-fat diet in black sea bream (Acanthopagrus schlegelii). An 8-week feeding trial was conducted on fish with an initial weight of 8·16 ± 0·01 g. Five diets were formulated: control, low-fat diet (11 %); HFD, high-fat diet (17 %); and HFD supplemented with graded levels of choline (3, 6 or 12 g/kg) termed HFD + C1, HFD + C2 and HFD + C3, respectively. Dietary choline decreased lipid content in whole body and tissues. Highest TAG and cholesterol concentrations in serum and liver were recorded in fish fed the HFD. Similarly, compared with fish fed the HFD, dietary choline reduced vacuolar fat drops and ameliorated HFD-induced pathological changes in liver. Expression of genes of lipolysis pathways were up-regulated, and genes of lipogenesis down-regulated, by dietary choline compared with fish fed the HFD. Expression of nfκb and pro-inflammatory cytokines in liver and intestine was suppressed by choline supplementation, whereas expression of anti-inflammatory cytokines was promoted in fish fed choline-supplemented diets. In fish that received lipopolysaccharide to stimulate inflammatory responses, the expression of nfκb and pro-inflammatory cytokines in liver, intestine and kidney were all down-regulated by dietary choline compared with the HFD. Overall, the present study indicated that dietary choline had a lipid-lowering effect, which could protect the liver by regulating intrahepatic lipid metabolism, reducing lipid droplet accumulation and suppressing NFκB activation, consequently attenuating HFD-induced inflammation in A. schlegelii.

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“…Besides, several studies recently showed that choline also exerted an anti-in ammatory effect. Dietary choline inhibited the expressions of proin ammatory biomarkers including TNF-α, IL-1β and NF-κB, while increased the abundance of anti-in ammatory cytokine genes in sh models [5,6]. To our knowledge, however, evidence to con rm the effect of dietary choline on the intestinal mucosal barrier function of mammal, especially weaned piglets was scarce.…”

Section: Introductionmentioning

confidence: 88%

Choline and Bile Acids Mixture Improved Growth Performance and Intestinal Immunity via Altering Gut Microbiota and Bacterial Metabolites in the Colon of Weaned Piglets.

Qiu

Liu

et al. 2020

Preprint

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Background: Choline or bile acids has many beneficial roles in physiological function. However, little was known about growth performance, intestinal mucosal function and microbiota-host interactions of weaned piglets in response to choline or bile acids supplementation. This study aimed to investigate the effect of choline and bile acids mixtures (ChB) supplementation on growth performance, intestinal mucosal barrier function, gut microbiota and bacterial metabolites of weaned piglets. One hundred and twenty-eight crossbred (Duroc × Landrace × Large White) weaned piglets (initial body weight: approximately 8 kg; 21 d of age) were randomly allocated to four different dietary treatments(a control diet (Control) and the other three groups were control diet supplemented with 800 mg/kg choline chloride (choline), 500 mg/kg bile acids (bile acids) or 800 mg/kg choline chloride plus 500 mg/kg bile acids (ChB), respectively) and for 28-d feeding trail. Results: ChB significantly increased average daily gain (ADG) and reduced feed/gain (F/G) ratio, associated with elevation of lipase activity and total bile acids level in ileal digesta compared with control diet. Additionally, ChB altered colonic microbiota by increasing the relative abundance of Lactobacillus and Faecalibacterium , and decreasing the relative abundances of unidentified-Clostridiales , Parabacteroides and Unidentified-Ruminococcaceae , when compared with control diet. Meanwhile, ChB increased the butyrate level and decreased the production of bile acid profiles in the colonic digesta. Besides, feeding ChB improved gut immunity, as reflected by increasing the abundance of IL-10 , FXR and mucin2 transcript, while downregulated expression of TLR4 , MyD88 , NF-κBp65 and TNF-α genes in the intestinal mucosa. Quantitative proteomics of jejunal mucosa further showed that ChB regulated the proteins that were related to inflammatory response. Furthermore, the changes in the ADG and genes expression were associated with alteration of gut microbiota composition and their metabolites. Conclusions: Collectively, our findings demonstrated that choline and bile acids mixture may improve the growth performance and intestinal immune response of weaned piglets through alteration of gut microbiota composition and bacterial metabolites, which promoted gut health.

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Dietary choline regulates antibacterial activity, inflammatory response and barrier function in the gills of grass carp (Ctenopharyngodon idella)

Cited by 35 publications

References 72 publications

Integrated omics analysis of sweat reveals an aberrant amino acid metabolism pathway in Vogt–Koyanagi–Harada disease

Integrated omics analysis of sweat reveals an aberrant amino acid metabolism pathway in Vogt–Koyanagi–Harada disease

Dietary choline supplementation attenuated high-fat diet-induced inflammation through regulation of lipid metabolism and suppression of NFκB activation in juvenile black seabream (Acanthopagrus schlegelii)

Choline and Bile Acids Mixture Improved Growth Performance and Intestinal Immunity via Altering Gut Microbiota and Bacterial Metabolites in the Colon of Weaned Piglets.

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