Expression analysis of ClpB/Hsp100 gene in faba bean (Vicia faba L.) plants in response to heat stress

Kumar, Ritesh; Singh, Amit Kumar; Lavania, Dhruv; Siddiqui, Manzer H.; Al-Whaibi, Mohamed H.; Grover, Anil

doi:10.1016/j.sjbs.2015.03.006

Cited by 18 publications

(8 citation statements)

References 19 publications

(22 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Analysis of Hsp100 in faba bean indicated upregulation of transcript level of ClpB/ Hsp100 gene under heat stress (38˚C) in leaf tissues. However, expression was below detection limit under control condition [30].…”

Section: Plos Onementioning

confidence: 91%

Phylogenetic and expression dynamics of tomato ClpB/Hsp100 gene under heat stress

et al. 2021

View full text Add to dashboard Cite

Heat shock proteins (Hsps) are stress-responsive molecular chaperones, which uphold proper protein folding in response to external and internal stresses. The Hsp100 gene family plays a substantial role in thermos-tolerance of plants. This study investigated evolutionary relationship and expression of ClpB/Hsp100 gene family in tomato under heat stress. Six SlHsp100 genes were identified using bioinformatics tools. In silico sub-cellular localization indicated that of these 6 ClpB/Hsp100 members, 4 are found in chloroplast, 1 in mitochondria and 1 in the cytoplasm. For evolutionary study, 36 SlHsp100 genes were included in the phylogenetic tree showing a hierarchical clustering shared by the members of the kingdoms Plantae, Archaea, Chromista, Fungi and Bacteria. A total 4 pairs of orthologous and 5 pairs of paralogous genes were identified. Functional divergence between different Hsp100 clusters showed considerable functional homology. Thermo-tolerance measured in terms of cell viability, cell membrane stability and pollen viability indicated that it was paralleled by thermal resistance of Hsps. Reverse transcriptase polymerase chain reaction was used to analyze gene expression in leaves of five-week-old tomato seedlings following exposure to heat stress (45°C) and control (25°C). Chloroplastic LeHSP110/ClpB gene was upregulated in all tomato genotypes after exposure to heat stress highlighting the crucial role of this gene family in acquired thermo-tolerance.

show abstract

Section: Plos Onementioning

confidence: 91%

Phylogenetic and expression dynamics of tomato ClpB/Hsp100 gene under heat stress

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Presence of ClpB/HSP100 protein was detected under heat stress in Phaseolus lunatus ( Keeler et al, 2000 ). It has been observed that accumulation of ClpB/HSP100 during high temperatures increased the pollen viability in faba bean ( Kumar et al, 2015b ). Recently, in faba bean VfHsp17.9-CII (a novel HSP protein) was identified which implements heat tolerance ( Kumar et al, 2015a ).…”

Section: Genetic Approaches For Heat Tolerance In Legumesmentioning

confidence: 99%

Food Legumes and Rising Temperatures: Effects, Adaptive Functional Mechanisms Specific to Reproductive Growth Stage and Strategies to Improve Heat Tolerance

et al. 2017

View full text Add to dashboard Cite

Ambient temperatures are predicted to rise in the future owing to several reasons associated with global climate changes. These temperature increases can result in heat stress- a severe threat to crop production in most countries. Legumes are well-known for their impact on agricultural sustainability as well as their nutritional and health benefits. Heat stress imposes challenges for legume crops and has deleterious effects on the morphology, physiology, and reproductive growth of plants. High-temperature stress at the time of the reproductive stage is becoming a severe limitation for production of grain legumes as their cultivation expands to warmer environments and temperature variability increases due to climate change. The reproductive period is vital in the life cycle of all plants and is susceptible to high-temperature stress as various metabolic processes are adversely impacted during this phase, which reduces crop yield. Food legumes exposed to high-temperature stress during reproduction show flower abortion, pollen and ovule infertility, impaired fertilization, and reduced seed filling, leading to smaller seeds and poor yields. Through various breeding techniques, heat tolerance in major legumes can be enhanced to improve performance in the field. Omics approaches unravel different mechanisms underlying thermotolerance, which is imperative to understand the processes of molecular responses toward high-temperature stress.

show abstract

“…The abundance of Hsp20, chaperone protein DnaJ homolog subfamily B member Os05g06440.1 and Hsp101 (Os05g44340) were upregulated across all the three genotypes under stress condition. Hsp101 is an important protein in imparting thermotolerance in bacteria, yeast and Arabidopsis ( Kumar et al, 2016 ; Kumar et al., 2020 ; Tiwari et al, 2021 ). The introduction of Arabidopsis thaliana Hsp101 (AtHsp101) into rice made the host variety more thermotolerant ( Katiyar-Agarwal et al., 2003 ).…”

Section: Discussionmentioning

confidence: 99%

Heat-induced proteomic changes in anthers of contrasting rice genotypes under variable stress regimes

Kumar

Ghatak²,

Goyal³

et al. 2023

Front. Plant Sci.

Self Cite

View full text Add to dashboard Cite

Heat stress drastically affects anther tissues resulting in poor plant fertility, necessitating an urgent need to determine the key proteome regulation associated with mature anther in response to heat stress. We identified several genotype - specific protein alterations in rice anthers of Moroberekan (Japonica, heat sensitive), IR64 (Indica, moderately heat tolerant), and Nagina22 (Aus, heat tolerant) in the short-term (ST_HS; one cycle of 42°C, 4 hours before anthesis) and long-term (LT_HS; 6 cycles of 38°C, 6 hours before anthesis) heat stress. The proteins upregulated in long-term heat stress in Nagina22 were enriched in biological processes related to unfolded protein binding and carboxylic acid metabolism, including amino acid metabolism. In short-term heat stress, Nagina22 anthers were enriched in proteins associated with vitamin E biosynthesis and GTPase activator activity. In contrast, downregulated proteins were related to ribosomal proteins. The expression of different Hsp20 and DnaJ was genotype specific. Overall, the heat response in Nagina22 was associated with its capacity for adequate metabolic control and cellular homeostasis, which may be critical for its higher reproductive thermotolerance. This study improves our understanding of thermotolerance mechanisms in rice anthers during anthesis and lays a foundation for breeding thermotolerant varieties via molecular breeding.

show abstract

Expression analysis of ClpB/Hsp100 gene in faba bean (Vicia faba L.) plants in response to heat stress

Cited by 18 publications

References 19 publications

Phylogenetic and expression dynamics of tomato ClpB/Hsp100 gene under heat stress

Phylogenetic and expression dynamics of tomato ClpB/Hsp100 gene under heat stress

Food Legumes and Rising Temperatures: Effects, Adaptive Functional Mechanisms Specific to Reproductive Growth Stage and Strategies to Improve Heat Tolerance

Heat-induced proteomic changes in anthers of contrasting rice genotypes under variable stress regimes

Contact Info

Product

Resources

About