Gold nanoparticles assisted laser desorption/ionization mass spectrometry and applications: from simple molecules to intact cells

Abdelhamid, Hani Nasser; Wu, Hui‐Fen

doi:10.1007/s00216-016-9374-6

Cited by 96 publications

(51 citation statements)

References 128 publications

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“…Another positive effect is that the spatial resolution depends only by instrumental specification like laser spot diameter and not by the crystallization of the matrix16. Metallic NPs have been largely employed in MALDI experiments1718192021 and in particular, TiO2 nanoparticles have been used for distribution studies of small endogenous molecules2223.…”

mentioning

confidence: 99%

3D Mass Spectrometry Imaging Reveals a Very Heterogeneous Drug Distribution in Tumors

Giordano

Morosi

Veglianese

et al. 2016

Sci Rep

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Mass Spectrometry Imaging (MSI) is a widespread technique used to qualitatively describe in two dimensions the distribution of endogenous or exogenous compounds within tissue sections. Absolute quantification of drugs using MSI is a recent challenge that just in the last years has started to be addressed. Starting from a two dimensional MSI protocol, we developed a three-dimensional pipeline to study drug penetration in tumors and to develop a new drug quantification method by MALDI MSI. Paclitaxel distribution and concentration in different tumors were measured in a 3D model of Malignant Pleural Mesothelioma (MPM), which is known to be a very heterogeneous neoplasm, highly resistant to different drugs. The 3D computational reconstruction allows an accurate description of tumor PTX penetration, adding information about the heterogeneity of tumor drug distribution due to the complex microenvironment. The use of an internal standard, homogenously sprayed on tissue slices, ensures quantitative results that are similar to those obtained using HPLC. The 3D model gives important information about the drug concentration in different tumor sub-volumes and shows that the great part of each tumor is not reached by the drug, suggesting the concept of pseudo-resistance as a further explanation for ineffective therapies and tumors relapse.

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mentioning

confidence: 99%

3D Mass Spectrometry Imaging Reveals a Very Heterogeneous Drug Distribution in Tumors

Giordano

Morosi

Veglianese

et al. 2016

Sci Rep

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“…Nanoparticles were suitable as MALDI matrices for small molecule imaging because of their uniform deposition over tissues (increasing lateral resolution) and the lack of background signals from matrix degradation. TiO 2 nanoparticles have been previously reported to be suitable for MALDI mass spectrometry analysis of low molecular weight compounds, with almost complete absence of background noise [40], and recently gold based nanoparticles have been reported to assist laser desorption/ionization, avoiding co-crystallization of the analyte and the matrix [41,42]. For the synthesis of AuNPs, we used a classical chemical reduction method that has been extensively used in the preparation of nanoparticles because this method is simple, cheap, and can be used to prepare large quantities of nanoparticles with an accurate shape and dimensions.…”

Section: Discussionmentioning

confidence: 99%

A Nanostructured Matrices Assessment to Study Drug Distribution in Solid Tumor Tissues by Mass Spectrometry Imaging

et al. 2017

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The imaging of drugs inside tissues is pivotal in oncology to assess whether a drug reaches all cells in an adequate enough concentration to eradicate the tumor. Matrix-Assisted Laser Desorption Ionization Mass Spectrometry Imaging (MALDI-MSI) is one of the most promising imaging techniques that enables the simultaneous visualization of multiple compounds inside tissues. The choice of a suitable matrix constitutes a critical aspect during the development of a MALDI-MSI protocol since the matrix ionization efficiency changes depending on the analyte structure and its physico-chemical properties. The objective of this study is the improvement of the MALDI-MSI technique in the field of pharmacology; developing specifically designed nanostructured surfaces that allow the imaging of different drugs with high sensitivity and reproducibility. Among several nanomaterials, we tested the behavior of gold and titanium nanoparticles, and halloysites and carbon nanotubes as possible matrices. All nanomaterials were firstly screened by co-spotting them with drugs on a MALDI plate, evaluating the drug signal intensity and the signal-to-noise ratio. The best performing matrices were tested on control tumor slices, and were spotted with drugs to check the ion suppression effect of the biological matrix. Finally; the best nanomaterials were employed in a preliminary drug distribution study inside tumors from treated mice.

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“…Recently, Abdelhamid and Wu [73] reviewed the applications of Au NPs assisted LDI-MS in various analytes including simple molecules and intact cells. However, Au NPs as a LDI matrix often produces many Au cluster peaks, which not only suppress the ionization of the analytes, but also result in complex mass spectrum.…”

Section: Nanomaterial-assisted Ldi Methods Developmentmentioning

confidence: 99%

Nanomaterials as Assisted Matrix of Laser Desorption/Ionization Time-of-Flight Mass Spectrometry for the Analysis of Small Molecules

Yang

et al. 2017

Nanomaterials

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Matrix-assisted laser desorption/ionization (MALDI), a soft ionization method, coupling with time-of-flight mass spectrometry (TOF MS) has become an indispensible tool for analyzing macromolecules, such as peptides, proteins, nucleic acids and polymers. However, the application of MALDI for the analysis of small molecules (<700 Da) has become the great challenge because of the interference from the conventional matrix in low mass region. To overcome this drawback, more attention has been paid to explore interference-free methods in the past decade. The technique of applying nanomaterials as matrix of laser desorption/ionization (LDI), also called nanomaterial-assisted laser desorption/ionization (nanomaterial-assisted LDI), has attracted considerable attention in the analysis of low-molecular weight compounds in TOF MS. This review mainly summarized the applications of different types of nanomaterials including carbon-based, metal-based and metal-organic frameworks as assisted matrices for LDI in the analysis of small biological molecules, environmental pollutants and other low-molecular weight compounds.

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Gold nanoparticles assisted laser desorption/ionization mass spectrometry and applications: from simple molecules to intact cells

Cited by 96 publications

References 128 publications

3D Mass Spectrometry Imaging Reveals a Very Heterogeneous Drug Distribution in Tumors

3D Mass Spectrometry Imaging Reveals a Very Heterogeneous Drug Distribution in Tumors

A Nanostructured Matrices Assessment to Study Drug Distribution in Solid Tumor Tissues by Mass Spectrometry Imaging

Nanomaterials as Assisted Matrix of Laser Desorption/Ionization Time-of-Flight Mass Spectrometry for the Analysis of Small Molecules

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