“…Nonomuraea gerenzanensis
[31] and Streptomyces coelicolor A3(2) working cell banks (WCBs) were prepared as previously described [29]. Preinoculum cultures were set up by inoculating 0.75 ml of the WCB into 15 ml VM liquid medium (in g/l: 24 soluble starch, 1 glucose, 3 meat extract, 5 yeast extract, and 5 tryptose) in 100 ml Erlenmeyer flasks, incubated at 28 °C and 200 revolutions per minute (rpm) for 72 h. An aliquot of 1.8 ml of these cultures was transferred into 300-ml baffled Erlenmeyer flasks containing 50 ml of two different basal media, VM and MM-L (liquid version, without agar), to which the following components could be added: 0.8 or 1.5 g/l alkali lignin, 6 or 12 g/l yeast extract, 2 mM MnCl 2 , 2 mM CuSO 4 , 0.2 mM FeSO 4 ·7H 2 O, 5 mM tryptophan, 0.5 g/l mannose, 0.5 g/l glucose, 6 g/l meat extract, 1 g/l hydrolyzed casein, and 3.5 or 5.0% v/v ethanol.…”