Expression of the energy substrate transporters in uterine fibroids

Knapp, Paweł; Chabowski, Adrian; Posmyk, Renata; Górski, Jan

doi:10.1016/j.prostaglandins.2016.02.002

Cited by 7 publications

(7 citation statements)

References 21 publications

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“…It is also known that fatty acid transporters content is highly coupled with FA transport. This phenomenon was observed in human breast cancer (Schimanski et al, 2010) and also in uterine fibroids (Knapp et al, 2016) with diminished FA transport proteins expression, which resulted in decreased FA transport into these cells. With regards to the potential mechanism of how AS160 knockdown could lead to an increase in the protein expression of FA transporters.…”

Section: Journal Of Cellular Physiologymentioning

confidence: 80%

Challenging of AS160/TBC1D4 Alters Intracellular Lipid milieu in L6 Myotubes Incubated With Palmitate

Mikłosz

Łukaszuk

Żendzian-Piotrowska

et al. 2017

Journal Cellular Physiology

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The Akt substrate of 160 kDa (AS160) is a key regulator of GLUT4 translocation from intracellular depots to the plasma membrane in myocytes. Likely, AS160 also controls LCFAs transport, which requires relocation of fatty acid transporters. The aim of the present study was to determine the impact of AS160 knockdown on lipid milieu in L6 myotubes incubated with palmitate (PA). Therefore, we compared two different settings, namely: 1) AS160 knockdown prior to palmitate incubation (pre‐PA‐silencing, AS160−/PA); 2) palmitate incubation with subsequent AS160 knockdown (post‐PA‐silencing, PA/AS160−). The efficiency of AS160 silencing was checked at mRNA and protein levels. The expression and localization of FA transporters were determined using Western Blot and immunofluorescence analyses. Intracellular lipid content (FFA, DAG, TAG, and PL) and FA composition were estimated by GLC, whereas basal palmitate uptake was analyzed by means of scintigraphy. Both groups with silenced AS160 were characterized by a greater expression of FA transporters (FAT/CD36, FATP‐1, 4) which had contributed to an increased FA cellular influx. Accordingly, we observed that post‐PA‐silencing of AS160 resulted in a marked decrement in DAG, TAG, and PL contents, but increased FFA content (PA/AS160− vs. PA). The opposite effect was observed in the group with pre‐PA‐silencing of AS160 in which AS160 knockdown did not affect the lipid pools (AS160−/PA vs. PA). Our results indicate that post‐PA‐silencing of AS160 has a capacity to decrease the lipotoxic effect(s) of PA by decreasing the content of lipids (DAG and PL) that promote insulin resistance in myotubes. J. Cell. Physiol. 232: 2373–2386, 2017. © 2016 The Authors. Journal of Cellular Physiology Published by Wiley Periodicals Inc.

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Section: Journal Of Cellular Physiologymentioning

confidence: 80%

Challenging of AS160/TBC1D4 Alters Intracellular Lipid milieu in L6 Myotubes Incubated With Palmitate

Mikłosz

Łukaszuk

Żendzian-Piotrowska

et al. 2017

Journal Cellular Physiology

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“…Our previous study demonstrated that in uterine leiomyomas the content of basic sphingolipids remains quite constant. However, the increase in CER level with a concomitant decrease in S1P concentration was observed as compared to normal uterus [22]. Based on our previous research we may also speculate that the neoplastic progression towards more aggressive neoplasms is related to the marked increase in sphingolipid content.…”

Section: Sphingolipid Metabolic Pathwaysmentioning

confidence: 89%

Unique Roles of Sphingolipids in Selected Malignant and Nonmalignant Lesions of Female Reproductive System

Knapp

Chomicz

Świderska

et al. 2019

BioMed Research International

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Cancer develops as a result of the loss of self-control mechanisms by a cell; it gains the ability to induce angiogenesis, becomes immortal and resistant to cell death, stops responding to growth suppressor signals, and becomes capable of invasion and metastasis. Sphingolipids—a family of membrane lipids—are known to play important roles in the regulation of cell proliferation, the response to chemotherapeutic agents, and/or prevention of cancer. Despite the underlying functions of sphingolipids in cancer biology, their metabolism in different malignant tumors is poorly investigated. Some studies showed marked differences in ceramide content between the tumor and the respective healthy tissue. Interestingly, the level of this sphingolipid could be either low or elevated, suggesting that the alterations in ceramide metabolism in cancer tissue may depend on the biology of the tumor. These processes are indeed related to the type of cancer, its stage, and histology status. In this paper we present the unique roles of bioactive sphingolipid derivative in selected gynecologic malignant and nonmalignant lesions.

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“…In order to determine the possible role of PGI2 in the porcine myometrium, tissue explants were exposed to iloprost, a stable analog of PGI2, and examined for mRNA transcript abundance of selected genes; in particular, genes involved in contractility, nutrient transport, inflammatory response, and PG synthesis and action were studied. These processes were previously reported as important for the proper function of the myometrium [ 7 , 8 , 10 , 11 , 45 ].…”

Section: Discussionmentioning

confidence: 99%

“…Cellular growth and activity require the supply of various nutrients. Myometrial smooth muscle cells use both glucose and fatty acids as major energy substrates [ 45 ]. Moreover, amino acids are important for the proper functioning of the myometrium [ 53 ].…”

Section: Discussionmentioning

confidence: 99%

Prostacyclin Synthesis and Prostacyclin Receptor Expression in the Porcine Myometrium: Prostacyclin Potential to Regulate Fatty Acid Transporters, Cytokines and Contractility-Related Factors

Blitek¹,

Łuba²,

Szymańska³

2022

Animals

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Although prostacyclin (PGI2) has been well described as a regulator of smooth muscle activity, limited data are available concerning its role in the myometrium of pigs. The present research aimed to examine profiles of PGI2 synthase (PTGIS) and PGI2 receptor (PTGIR) expression and 6-keto PGF1α (a PGI2 metabolite) concentrations in the myometrium of gilts throughout the estrous cycle and during early pregnancy using qPCR, Western blot, and/or ELISA methods. Furthermore, myometrial explants were exposed to iloprost (a stable PGI2 analog) to investigate the effect of PGI2 on the mRNA expression of factors engaged in smooth muscle contraction, nutrient transport, prostaglandin synthesis and action, and inflammatory response. PTGIS mRNA expression was greater in cyclic than in pregnant gilts on days 11–12 after estrus and was accompanied by greater concentrations of 6-keto PGF1α detected in cyclic than in pregnant animals on days 11–20. Iloprost stimulated fatty acid transporters and contractility-related calponin 1 and caldesmon 1 mRNA expression and decreased interleukin 1β and tumor necrosis factor transcript abundance. The obtained results indicate a physiologically relevant role of PGI2 during the estrous cycle in the porcine myometrium with its importance for regulating the expression of contractility-, nutrient transport- and inflammatory response-related factors.

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Expression of the energy substrate transporters in uterine fibroids

Cited by 7 publications

References 21 publications

Challenging of AS160/TBC1D4 Alters Intracellular Lipid milieu in L6 Myotubes Incubated With Palmitate

Challenging of AS160/TBC1D4 Alters Intracellular Lipid milieu in L6 Myotubes Incubated With Palmitate

Unique Roles of Sphingolipids in Selected Malignant and Nonmalignant Lesions of Female Reproductive System

Prostacyclin Synthesis and Prostacyclin Receptor Expression in the Porcine Myometrium: Prostacyclin Potential to Regulate Fatty Acid Transporters, Cytokines and Contractility-Related Factors

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