Influence of moderate hypoxia on vaccine efficacy against Vibrio anguillarum in Oreochromis niloticus (Nile tilapia)

Gallage, Sanchala; Katagiri, Takayuki; Endo, Makoto; Futami, Kunihiko; Maita, Masashi

doi:10.1016/j.fsi.2016.02.024

Cited by 56 publications

(17 citation statements)

References 46 publications

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“…For example, chronic and moderate intermittent hypoxia have been shown to decrease leucocyte respiratory burst in Atlantic salmon (42). Gallage et al (117) observed a lower, and delayed, antibody production in Nile tilapia chronically exposed to hypoxia, and the authors associated the delay in antibody production with a reduction in the transcript levels of i1β, tcrβ, and mhcIIβ. Kvamme et al (43) found that Atlantic salmon exposed to chronic hypoxia had either reduced expression or a delay in the expression of immune-related genes.…”

Section: The Overall Effects Of An Incremental Temperature Increase Amentioning

confidence: 99%

The Innate Immune Response of Atlantic Salmon (Salmo salar) Is Not Negatively Affected by High Temperature and Moderate Hypoxia

et al. 2020

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Climate change is predicted to increase water temperatures and decrease oxygen levels in freshwater and marine environments, however, there is conflicting information regarding the extent to which these conditions may impact the immune defenses of fish. In this study, Atlantic salmon were exposed to: (1) normoxia (100-110% air saturation) at 12 • C; (2) an incremental temperature increase (1 • C per week from 12 to 20 • C), and then held at 20 • C for an additional 4 weeks; and (3) "2" with the addition of moderate hypoxia (∼65-75% air saturation). These conditions realistically reflect what farmed salmon in some locations are currently facing, and future conditions in Atlantic Canada and Europe, during the summer months. The salmon were sampled for the measurement of head kidney constitutive anti-bacterial and anti-viral transcript expression levels, and blood parameters of humoral immune function. Thereafter, they were injected with either the multi-valent vaccine Forte V II (contains both bacterial and viral antigens) or PBS (phosphate-buffer-saline), and the head kidney and blood of these fish were sampled at 6, 12, 24, and 48 h post-injection (HPI). Our results showed that: (1) neither high temperature, nor high temperature + moderate hypoxia, adversely affected respiratory burst, complement activity or lysozyme concentration; (2) the constitutive transcript expression levels of the anti-bacterial genes il1β, il8-a, cox2, hamp-a, stlr5-a, and irf7-b were up-regulated by high temperature; (3) while high temperature hastened the peak in transcript expression levels of most anti-bacterial genes by 6-12 h following V II injection, it did not affect the magnitude of changes in transcript expression; (4) anti-viral (viperin-b, mx-b, and isg15-a) transcript expression levels were either unaffected, or downregulated, by acclimation temperature or V II injection over the 48 HPI; and (5) hypoxia, in addition to high temperature, did not impact immune transcript expression. In conclusion, temperatures up to 20 • C, and moderate hypoxia, do not impair the capacity of the Atlantic salmon's innate immune system to respond to bacterial antigens. These findings are surprising, and highlight the salmon's capacity to mount robust innate immune responses (i.e., similar to control fish under optimal conditions) under conditions approaching their upper thermal limit.

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Section: The Overall Effects Of An Incremental Temperature Increase Amentioning

confidence: 99%

The Innate Immune Response of Atlantic Salmon (Salmo salar) Is Not Negatively Affected by High Temperature and Moderate Hypoxia

et al. 2020

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“…Currently, little hypoxia related molecular data in this fish is available. In tilapia, acute hypoxia triggered an overall down-regulation of the immune system [39] and moderate hypoxia negatively affects antibody production, ultimately affecting vaccine efficacy [40]. The depression of whole animal oxygen consumption rate, heart rate and cardiac output were found contribute to hypoxic survival [41].…”

Section: Introductionmentioning

confidence: 99%

Characterization and functional analysis of hypoxia-inducible factor HIF1α and its inhibitor HIF1αn in tilapia

et al. 2017

PLoS ONE

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Hypoxia is a major cause of fish morbidity and mortality in the aquatic environment. Hypoxia-inducible factors are very important modulators in the transcriptional response to hypoxic stress. In this study, we characterized and conducted functional analysis of hypoxia-inducible factor HIF1α and its inhibitor HIF1αn in Nile tilapia (Oreochromis niloticus). By cloning and Sanger sequencing, we obtained the full length cDNA sequences for HIF1α (2686bp) and HIF1αn (1308bp), respectively. The CDS of HIF1α includes 15 exons encoding 768 amino acid residues and the CDS of HIF1αn contains 8 exons encoding 354 amino acid residues. The complete CDS sequences of HIF1α and HIF1αn cloned from tilapia shared very high homology with known genes from other fishes. HIF1α show differentiated expression in different tissues (brain, heart, gill, spleen, liver) and at different hypoxia exposure times (6h, 12h, 24h). HIF1αn expression level under hypoxia is generally increased (6h, 12h, 24h) and shows extremely highly upregulation in brain tissue under hypoxia. A functional determination site analysis in the protein sequences between fish and land animals identified 21 amino acid sites in HIF1α and 2 sites in HIF1αn as significantly associated sites (α = 0.05). Phylogenetic tree-based positive selection analysis suggested 22 sites in HIF1α as positively selected sites with a p-value of at least 95% for fish lineages compared to the land animals. Our study could be important for clarifying the mechanism of fish adaptation to aquatic hypoxia environment.

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“…Previous findings by Gallage et al. (2006) demonstrated that fish vaccinated with S . agalactiae and exposed to moderate hypoxic conditions could have insufficient phagocytosis against the bacterium.…”

Section: Resultsmentioning

confidence: 88%

Prevention and Control of Streptococcosis in Tilapia Culture: A Systematic Review

Maulu¹,

Hasimuna

Mphande

et al. 2021

J Aqua Anim Hlth

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Tilapia culture is a very promising industry within the aquaculture sector. However, disease outbreaks have continued to threaten the industry, causing serious economic losses among the producers. Streptococcosis has become the major bacterial disease affecting tilapia production in most regions of the world. To combat the disease and minimize its economic impact on fish producers, numerous preventive and control measures have been developed and reported over the years. This paper aims to systematically review the measures that could be used to manage the disease outbreaks and maintain fish health based on previously published scientific studies. Although numerous measures currently available have been highlighted, it is far better for the producers to maximize the preventive measures for management to be economically feasible. Among the currently available preventive measures, the use of vaccines has been shown to have the most promise, while the use of herbs has been demonstrated to be a more sustainable and economically affordable control measure. However, there are still a number of important gaps in existing literature that require further investigation. Overall, significant progress has been made in preventing and controlling streptococcosis in tilapia although, no single effective measure has been identified. Therefore, a combination of these measures may provide a more effective result.

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Influence of moderate hypoxia on vaccine efficacy against Vibrio anguillarum in Oreochromis niloticus (Nile tilapia)

Cited by 56 publications

References 46 publications

The Innate Immune Response of Atlantic Salmon (Salmo salar) Is Not Negatively Affected by High Temperature and Moderate Hypoxia

The Innate Immune Response of Atlantic Salmon (Salmo salar) Is Not Negatively Affected by High Temperature and Moderate Hypoxia

Characterization and functional analysis of hypoxia-inducible factor HIF1α and its inhibitor HIF1αn in tilapia

Prevention and Control of Streptococcosis in Tilapia Culture: A Systematic Review

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