Transcriptome analysis and anthocyanin-related genes in red leaf lettuce

Zhang, Y Z; Xu, Shuzhen; Cheng, Yanwei; Ya, Huiyuan; Han, Jianming

doi:10.4238/gmr.15017023

Cited by 30 publications

(23 citation statements)

References 31 publications

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“…In this study, expression of the MYB10 gene was high, and correlated with elevated anthocyanin content in red-leaf peach (Figures 11 and 12). This is consistent with the results of previous studies (Ban et al, 2007;Wan et al, 2015;Zhang et al, 2016), in which MYB-like genes have been found to positively regulate the synthesis and accumulation of anthocyanin.…”

Section: Discussionsupporting

confidence: 82%

Cloning and bioinformatic analysis of transcription factor MYB10 from the red-leaf peach

et al. 2016

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ABSTRACT. In higher plants, the transcription factor MYB10 is an important regulator of anthocyanin biosynthesis. In order to study its role in the development of red coloration in peach leaves, the full-length MYB10 complementary DNA sequence of the red-leaf peach cultivar 'Tsukuba No. 5' (Prunus persica f. atropurpurea) was successfully cloned using reverse transcription-polymerase chain reaction. The sequence was assigned the GenBank accession No. KP315904. Bioinformatic analysis identified the complete MYB10 open reading frame, consisting of 678 bp encoding 225 amino acids. The predicted protein has a molecular weight of 26.56 kDa and a theoretical isoelectric point of 8.97. The secondary structure was found to comprise approximately 34.22% alpha helix, 15.11% extended strand, 10.67% beta turn, and 40% random coil. Subcellular analysis indicated that MYB10 may function in the cytoplasm. Assessment of the amino acid sequence suggested the presence of one serine and two threonine phosphorylation sites. Quantitative real-time polymerase chain reaction revealed that MYB10 expression positively correlated with anthocyanin content in red-leaf peach, indicating that this transcription factor plays a role in the biosynthesis of this pigment in peach trees.

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Section: Discussionsupporting

confidence: 82%

Cloning and bioinformatic analysis of transcription factor MYB10 from the red-leaf peach

et al. 2016

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“…Comparative transcriptome analysis by RNA-Seq has been effectively used for investigating the genes involved in anthocyanin biosynthesis in several plants, such as Vitis vinifera [27], Solanum melongena L. [29], Litchi chinensis Sonn. [48], and Lactuca sativa [49]. In the present study on L. ruthenicum, a total of 43,573 unigenes were assembled, of which only 54.44% were identified (S2 Table).…”

Section: Discussionmentioning

confidence: 94%

Transcriptomic analysis of Lycium ruthenicum Murr. during fruit ripening provides insight into structural and regulatory genes in the anthocyanin biosynthetic pathway

Duan

Zhang

et al. 2018

PLoS ONE

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Fruit development in Lycium ruthenicum Murr. involves a succession of physiological and biochemical changes reflecting the transcriptional modulation of thousands of genes. Although recent studies have investigated the dynamic transcriptomic responses during fruit ripening in L. ruthenicum, most have been limited in scope, and thus systematic data representing the structural genes and transcription factors involved in anthocyanin biosynthesis are lacking. In this study, the transcriptomes of three ripening stages associated with anthocyanin accumulation, including S1 (green ripeness stage), S2 (skin color change) and S3 (complete ripeness stage) in L. ruthenicum were investigated using Illumina sequencing. Of a total of 43,573 assembled unigenes, 12,734 were differentially expressed during fruit ripening in L. ruthenicum. Twenty-five significantly differentially expressed structural genes (including PAL, C4H, 4CL, CHS, CHI, F3H, F3'H, F3'5'H, DFR, ANS and UFGT) were identified that might be associated with anthocyanin biosynthesis. Additionally, several transcription factors, including MYB, bHLH, WD40, NAC, WRKY, bZIP and MADS, were correlated with the structural genes, implying their important interaction with anthocyanin biosynthesisrelated genes. Our findings provide insight into anthocyanin biosynthesis and regulation patterns in L. ruthenicum and offer a systematic basis for elucidating the molecular mechanisms governing anthocyanin biosynthesis in L. ruthenicum.

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“…This red color was caused by increased anthocyanin accumulation. Zhang et al (2016a) previously reported several putative genes involved in the anthocyanin biosynthetic pathway. However, the molecular mechanisms governing light-induced anthocyanins in red leaf lettuce are still unknown.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome profiling of anthocyanin-related genes reveals effects of light intensity on anthocyanin biosynthesis in red leaf lettuce

Zhang

Cheng

et al. 2018

PeerJ

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Red leaf lettuce (Lactuca sativa L.) is popular due to its high anthocyanin content, but poor leaf coloring often occurs under low light intensity. In order to reveal the mechanisms of anthocyanins affected by light intensity, we compared the transcriptome of L. sativa L. var. capitata under light intensities of 40 and 100 μmol m−2 s−1. A total of 62,111 unigenes were de novo assembled with an N50 of 1,681 bp, and 48,435 unigenes were functionally annotated in public databases. A total of 3,899 differentially expressed genes (DEGs) were detected, of which 1,377 unigenes were up-regulated and 2,552 unigenes were down-regulated in the high light samples. By Kyoto Encyclopedia of Genes and Genomes enrichment analysis, the DEGs were significantly enriched in 14 pathways. Using gene annotation and phylogenetic analysis, we identified seven anthocyanin structural genes, including CHS, CHI, F3H, F3′H, DFR, ANS, and 3GT, and two anthocyanin transport genes, GST and MATE. In terms of anthocyanin regulatory genes, five MYBs and one bHLH gene were identified. An HY5 gene was discovered, which may respond to light-signaling and regulate anthocyanin structural genes. These genes showed a log2FC of 2.7–9.0 under high irradiance, and were validated using quantitative real-time-PCR. In conclusion, our results indicated transcriptome variance in red leaf lettuce under low and high light intensity, and observed a anthocyanin biosynthesis and regulation pattern. The data should further help to unravel the molecular mechanisms of anthocyanins influenced by light intensity.

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Transcriptome analysis and anthocyanin-related genes in red leaf lettuce

Cited by 30 publications

References 31 publications

Cloning and bioinformatic analysis of transcription factor MYB10 from the red-leaf peach

Cloning and bioinformatic analysis of transcription factor MYB10 from the red-leaf peach

Transcriptomic analysis of Lycium ruthenicum Murr. during fruit ripening provides insight into structural and regulatory genes in the anthocyanin biosynthetic pathway

Transcriptome profiling of anthocyanin-related genes reveals effects of light intensity on anthocyanin biosynthesis in red leaf lettuce

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