Transfer of useful variability of high grain iron and zinc from<i>Aegilops kotschyi</i>into wheat through seed irradiation approach

Verma, Shreya; Kumar, Satish; Sheikh, Imran; Malik, Sachin; Mathpal, Priyanka; Chugh, Vishal; Kumar, Sunil; Prasad, Ramasare; Dhaliwal, H. S.

doi:10.3109/09553002.2016.1135263

Cited by 27 publications

(12 citation statements)

References 36 publications

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“…The number of reports related to biofortified crops have increased in the recent past due to their imperative social impact, dietary needs, and market potential (De Steur et al 2015;Verma et al 2016). Most of the commercial wheat cultivars have poor grain Fe and Zn content, whereas the related wild species including Ae.…”

Section: Discussionmentioning

confidence: 99%

“…A majority of the QTL for grain Fe and Zn content have been mapped on groups 2 and 7 chromosomes of wheat, and 2S and 7U chromosomes had superior genetic system for micronutrient uptake, transport, and storage Tiwari et al 2010;Verma et al 2016). Several genes responsible for iron and zinc transport have been mapped on group 2 chromosomes of wheat.…”

Section: Discussionmentioning

confidence: 99%

“…The high purity and ICPMS grade Fe and Zn standards of Merck were used in this study. The grain Fe and Zn data of BC 2 F 1 and BC 2 F 2 generations was statistically analyzed through Student's t test to analyze the significance of difference among both the parents and wheat-Aegilops interspecific derivatives (Verma et al 2016).…”

Section: Biochemical Methodsmentioning

confidence: 99%

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Induced Homoeologous Pairing for Transfer of Useful Variability for High Grain Fe and Zn from Aegilops kotschyi into Wheat

et al. 2016

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Biofortification of bread wheat by the transfer of useful variability of high grain Fe and Zn from Aegilops kotschyi through induced homoeologous pairing is the most feasible approach to alleviate micronutrient malnutrition worldwide. Deficiency of chromosome 5B in interspecific hybrids allows homoeologous pairing and recombination of chromosomes of wheat with those of the related species. The interspecific hybrid plants without 5B chromosome showed much higher chromosome pairing than did the plants with 5B. The F 1 plants without 5B chromosome were selected and repeatedly backcrossed with wheat cultivar PBW343. The chromosome number of BC 2 F 1 plants ranged from 43 to 60 with several univalents and multivalents. Molecular markers and GISH analysis confirmed the introgression of U/S chromosomes of Ae. kotschyi and their fragments in wheat. The BC 2 F 2 plants showed up to 125 % increase in Fe and 158 % increase in Zn compared to PBW343 with Lr24 and Yr36. Induced homoeologous pairing in the absence of 5B was found to be an effective approach for transfer of useful variability for enhanced grain Fe and Zn content for biofortification of wheat for high grain micronutrient content.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Induced Homoeologous Pairing for Transfer of Useful Variability for High Grain Fe and Zn from Aegilops kotschyi into Wheat

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“…kotschyi addition lines were used for enhancement of grain protein, Fe and Zn content (Verma et al 2016a). The use of irradiated seed is an alternative approach for precise gene transfer from related species of wheat and mapping (Verma et al 2016b). In wheat, radiation hybrids have been used for transfer of genes controlling high grain iron and zinc (Verma et al 2016a, b).…”

Section: Introductionmentioning

confidence: 99%

Transfer of HMW glutenin subunits from Aegilops kotschyi to wheat through radiation hybridization

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High molecular weight glutenin subunits (HMWGS) are responsible for dough elasticity and bread making quality of bread wheat. Related wild non-progenitor species, Aegilops kotschyi possesses higher molecular weight x and y glutenin subunits than the bread wheat cultivars. A wheat-Aegilops substitution line with 1U chromosome was used for the transfer of (HMWGS) of 1U to wheat by using pollen radiation hybridization approach. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis profiling showed different patterns of allelic variations with either the presence or absence of HMWGS, Glu-1A (1, null), Glu-1B (7, 7 ? 8, 17 ? 18) and Glu-1D (5 ? 10, 2 ? 12, null). The pollen irradiated wheat-Aegilops derivatives, B-56-1-4-2, B-56-1-4-3, B-14-1 and B-14-2 with Glu1Ux and 1Uy and absence or presence of some Glu-1A and Glu-1B HMWGS showed high micro SDS sedimentation test (MST) values while B-16-1 and B-16-2 had moderate MST values and high protein content. However, B-58-3 with transfer of Glu-1Ux ? 1Uy for Glu-1D showed very low MST values indicating that Glu1Ux ? 1Uy enhance MST value only in the presence of Glu1D HMWGS. The transfer/substitution of alien HMW-GS for Glu-1A and or Glu-1B loci only can lead to improved bread making quality of wheat.

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“…Related progenitor and non-progenitor Triticeae and other species with a vast reservoir of useful variability have been extensively utilized for the development of wheatalien addition and substitution lines. The U and S genomes of Aegilops possess superior genetic systems for abiotic stress tolerance, grain micronutrient content, and disease and pest resistance (Verma et al 2016a;Wulff and Moscou 2014) and have a syntenic relationship with ABD genomes of wheat (Middleton et al 2014;Molnar et al 2013). However, precise gene transfer of useful variability without linkage drag is the major bottleneck to exploiting germplasm of related non-progenitor species.…”

Section: Introductionmentioning

confidence: 99%

Transferability and Polymorphism Between Group 7 Chromosome Specific Simple Sequence Repeat (SSR) Markers of Bread Wheat and Its Related Non-ProgenitorAegilopsSpecies

Kumar

Verma

Kundu

et al. 2016

Journal of Crop Improvement

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Simple sequence repeat (SSR) markers are highly efficient for genetic mapping and molecular breeding in crop plants. Chromosomes 7S and 7U of related non-progenitor Aegilops species possess superior genetic systems for high grain iron and zinc content. This study was undertaken to conduct a comprehensive analysis of transferability and polymorphism among group-7 anchored SSR markers of bread wheat (Triticum aestivum L.) to Aegilops species for wheat improvement. The study revealed 77% transferability of group 7-specific SSR markers of bread wheat to 7U/7S chromosomes of Aegilops species. More than 80% of the 7D specific markers were found to be transferable with a high level of polymorphism. The transferability to 7S and 7U genome suggested higher similarity between bread wheat genome(s) and S genome as compared with U genome of Aegilops species. These polymorphic markers are highly informative, robust and cost-effective for molecular breeding and could be further utilized for identification of introgression/transfer(s) of 7S/7U chromosomal fragments in bread wheat genome through wide hybridization. ARTICLE HISTORY

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Transfer of useful variability of high grain iron and zinc fromAegilops kotschyiinto wheat through seed irradiation approach

Abstract: Radiation-induced hybrids showed more than 65% increase in grain iron and 54% increase in Zn contents with better harvest index than the elite wheat cultivar WL711 indicating effective and compensating translocations of 2S(k) fragments into wheat genome.

Cited by 27 publications

References 36 publications

Induced Homoeologous Pairing for Transfer of Useful Variability for High Grain Fe and Zn from Aegilops kotschyi into Wheat

Induced Homoeologous Pairing for Transfer of Useful Variability for High Grain Fe and Zn from Aegilops kotschyi into Wheat

Transfer of HMW glutenin subunits from Aegilops kotschyi to wheat through radiation hybridization

Transferability and Polymorphism Between Group 7 Chromosome Specific Simple Sequence Repeat (SSR) Markers of Bread Wheat and Its Related Non-ProgenitorAegilopsSpecies

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