TheVviMYB80Gene is Abnormally Expressed inVitis viniferaL. cv. ‘Zhong Shan Hong’ and its Expression in Tobacco Driven by the 35S Promoter Causes Male Sterility

Abstract

Anther development is a very precise and complicated process. In Arabidopsis, the AtMYB80 transcription factor regulates genes involved in pollen development and controls the timing of tapetal programmed cell death (PCD). In this study, we isolated and characterized cDNA for VviMYB80 expressed in flower buds of male-sterile Vitis vinifera L. cv. 'Zhong Shan Hong', a late-maturing cultivar derived from self-progeny of cv. 'Wink'. VviMYB80 belongs to the MYB80 subfamily and clusters with AtMYB35/TDF1 in a distinct clade. We found that in flower buds, expression of the VviMYB80 gene in cv. 'Zhong Shan Hong' sharply increased at the tetrad stage, resulting in a higher and earlier transcript level than that found in cv. 'Wink'. Expression of the VviMYB80 gene, driven by the 35S promoter, caused pleiotropic effects on the stamens, including smaller and shriveled anthers, delayed dehiscence, fewer seeds, shorter anther filaments, distorted pollen shape and a lack of cytoplasm, with the tapetum exhibiting hypertrophy in transformed tobacco. These results suggest that VviMYB80 may play an important role in stamen development and that expression of VviMYB80 driven by the 35S promoter in tobacco induces male sterility.

A systems-oriented analysis of the grapevine R2R3-MYB transcription factor family uncovers new insights into the regulation of stilbene accumulation

“…Grapevine MYBA1-A2 regulate anthocyanin accumulation by modulating UDP-GLUCOSE:FLAVONOID 3-O-GLUCOSYLTRANSFERASE ( UFGT ) expression. 13 We were able to associate eight MYBA genes ( MYBA1 , MYBA2 , MYBA3 , MYBA7 , MYBA9 , MYBA10 , MYBA11 and MYBA12 ) and four additional MYB TFs ( MYB108B , MYB15 , MYBPA10 and MYB142 ) with anthocyanin-related genes. For example, MYBA1-2 were co-expressed with UFGT (VIT_16s0039g02230 ) while MYB108B was co-expressed with ANTHOCYANIN GLUTATHIONE S-TRANSFERASE 4 (GST4, VIT_04s0079g00690 ), the gene involved in anthocyanin transport into vacuoles.…”

“…Despite its size, less than 10% of the members of this family have been functionally characterized. With the exception of VviMYB60 (regulating stomata aperture 12 ) and VviMYB80 (controlling stamen development, 13 all other R2R3-MYBs characterized to date are involved in regulating the phenylpropanoid pathway. These include activators of the anthocyanin, 14 , 15 flavonoid, 16–18 proanthocyanidin (PA) 19 and stilbene 20 pathways, and also members of the C2-repressor motif clade.…”

Identification and characterization of PsDREB2 promoter involved in tissue-specific expression and abiotic stress response from Paeonia suffruticosa

“…Constitutive promoters can initiate the expression of target genes in various plant tissues, and gene transcription is persistent without space–time specificity. Currently, in plant genetic engineering, the most commonly used constitutive promoter is CaMV35S .This promoter has been widely applied (Okayama et al, 2010; Yang et al, 2015; Zheng et al, 2016; Cai, Song & Zhang, 2016; Azizi et al, 2016; Testroet et al, 2017) but shows some drawbacks. CaMV35S induces constant and continuous gene expression during the whole plant growth period in various tissues and organs.…”

Evaluation of mitotic activity in tapetal cells of grapevine (Vitis L.)

“…Several transcription factors and genes have been reported to be associated with the proper tapetum development and degeneration. Studies developed in the model plant Arabidopsis thaliana demonstrated that the mutation in members of LLR receptor kinase EMS1signaling complex resulted in impairing tapetal cells proliferation (Feng and Dickinson, 2010) while the mutant gene ms1 caused a delay of tapetum degeneration (Quilichini et al, 2014) and the mutant gene myb80 gave rise to a precocious tapetum degeneration (Zheng et al, 2016). In rice, it was observed that the transcription factor EAT1positively regulated the tapetum PCD and triggers the phasiRNA biogenesis that was exported to PMCs during early meiosis.…”