Multidrug resistant, extended spectrum β-lactamase (ESBL)-producingEscherichia coliisolated from a dairy farm

Abstract

Escherichia coli strains were isolated from a single dairy farm as a sentinel organism for the persistence of antibiotic resistance genes in the farm environment. Selective microbiological media were used to obtain 126 E. coli isolates from slurry and faeces samples from different farm areas. Antibiotic resistance profiling for 17 antibiotics (seven antibiotic classes) showed 57.9% of the isolates were resistant to between 3 and 15 antibiotics. The highest frequency of resistance was to ampicillin (56.3%), and the lowest to imipenem (1.6%), which appeared to be an unstable phenotype and was subsequently lost. Extended spectrum β-lactamase (ESBL) resistance was detected in 53 isolates and blaCTX-M, blaTEM and blaOXA genes were detected by PCR in 12, 4 and 2 strains, respectively. Phenotypically most isolates showing resistance to cephalosporins were AmpC rather than ESBL, a number of isolates having both activities. Phenotypic resistance patterns suggested co-acquisition of some resistance genes within subsets of the isolates. Genotyping using ERIC-PCR demonstrated these were not clonal, and therefore co-resistance may be associated with mobile genetic elements. These data show a snapshot of diverse resistance genes present in the E. coli population reservoir, including resistance to historically used antibiotics as well as cephalosporins in contemporary use.

Pola Resistensi Antibiotik pada Escherichia coli Penghasil ESBL dari Sampel Lingkungan di RPH-R Kota Bogor

“…Hal ini menjadi masalah kesehatan global karena perkembangannya dapat menyebabkan bakteri menjadi resisten terhadap antibiotik. Kotoran hewan menjadi sumber utama bagi bakteri yang telah resisten untuk menyebar ke lingkungan, khususnya tanah (Ibrahim et al, 2016). Penggunaan obat-obatan, termasuk antibiotik, dalam peternakan tidak dapat dihindarkan.…”

“…Keranjang terkontaminasi feses maupun darah dari proses produksi sebelumnya. Mengingat bahwa mikrob yang berada di dalam feses atau kotoran sapi dapat bermigrasi ke lingkungan di sekitarnya (Ibrahim et al, 2016). Sumber kontaminasi di rumah potong hewan dapat berasal dari tanah sekitar, kulit, rambut, isi saluran pencernaan, darah, air, peralatan, dan fasilitas yang digunakan selama proses produksi (seperti pisau, katrol, pengait, dan peralatan di tempat jeroan), kotoran, udara, dan pekerja (Hansson, 2001;Sofos, 2008).…”

Detection of Ampicillin Resistance Encoding Gene of Escherichia coli from Chickens in Bandung and Purwakarta

“…E. coli is known to show resistance to several types of antibiotics. Bacteria that naturally have antibiotic resistant genes can act as the reservoir by transferring these genes to other bacteria (Ibrahim et al 2016). The nature of resistance can be transferred through a mechanism called horizontal gene transfer to other bacteria including pathogenic bacteria, and it ultimately can affect on human health.…”

Ceasing the use of the highest priority critically important antimicrobials does not adversely affect production, health or welfare parameters in dairy cows

“…A recent review of published literature highlighted that, for some bacteria, AMR patterns seen in humans could be a result of antimicrobial (AM) use in livestock (1). There are also increasing reports of resistant bacteria being recovered from production animals (2)(3)(4)(5) and evidence that the use of certain classes of antibiotics in livestock increase the risks of multiresistant bacteria being present on farms using these medicines (6,7).…”