The effects of Lycii Radicis Cortex on RANKL-induced osteoclast differentiation and activation in RAW 264.7 cells

Abstract

Post-menopausal osteoporosis is a serious age-related disease. After the menopause, estrogen deficiency is common, and excessive osteoclast activity causes osteoporosis. Osteoclasts are multinucleated cells generated from the differentiation of monocyte/macrophage precursor cells such as RAW 264.7 cells. The water extract of Lycii Radicis Cortex (LRC) is made from the dried root bark of Lycium chinense Mill. and is termed 'Jigolpi' in Korea. Its effects on osteoclastogenesis and post-menopausal osteoporosis had not previously been tested. In the present study, the effect of LRC on receptor activator of nuclear factor-κB (NF-κB) ligand (RANKL)-induced osteoclast differentiation was demonstrated using a tartrate-resistant acid phosphatase (TRAP) assay and pit formation assay. Moreover, in order to analyze molecular mechanisms, we studied osteoclastogenesis-related markers such as nuclear factor of activated T-cells cytoplasmic 1 (NFATc1), c-Fos, receptor activator of NF-κB (RANK), TRAP, cathepsin K (CTK), matrix metallopeptidase-9 (MMP-9), calcitonin receptor (CTR) and carbonic anhydrase II (CAII) using RT-qPCR and western blot analysis. Additionally, we also determined the effect of LRC on an ovariectomized (OVX) rat model. We noted that LRC inhibited RANKL-induced osteoclast differentiation via suppressing osteoclastogenesis-related markers. It also inhibited osteoporosis in the OVX rat model by decreasing loss of bone density and trabecular area. These results suggest that LRC exerts a positive effect on menopausal osteoporosis.

Effects of interleukin‐7/interleukin‐7 receptor on RANKL‐mediated osteoclast differentiation and ovariectomy‐induced bone loss by regulating c‐Fos/c‐Jun pathway

“…Osteoclasts are multinucleated, terminally differentiated cells found in bone tissues, which are difficult to culture in vitro, while in recent years, RANKL has been widely and successfully used to induce the differentiation and maturation of osteoclast precursors into osteoclasts (Kim et al, ; Wang, Zheng, et al, ). Therefore, we used RANKL to induce osteoclastogenesis of RAW264.7 cells and BMMs in vitro, and our results revealed that IL‐7 significantly up‐regulated the expression of IL‐7R and activated the c‐Fos/c‐Jun signaling pathway.…”

Protective Effects of 2,3,5,4′-Tetrahydroxystilbene-2-O-β-d-glucoside on Ovariectomy Induced Osteoporosis Mouse Model

“…Sex hormones influence body fat distribution and adipocyte differentiation. Some researchers have reported that an estrogen deficiency may be an important obesity-triggering factor [ 44 ], and several studies have reported that estrogen deficiency increases the body weight of mice [ 45 , 46 , 47 ]. In the present study, we showed that the administration of TSG effectively inhibited weight gain by estrogen deficiency.…”

“…These results suggest that TSG may prevent disease associated with increased body weight in postmenopausal women. Moreover, estrogen deficiency induces atrophy of the uterus, and uterine atrophy is evidence of the success of ovariectomy [ 45 ]. In the present study, we demonstrated that TSG reduced uterine atrophy.…”

Kukoamine A inhibits human glioblastoma cell growth and migration through apoptosis induction and epithelial-mesenchymal transition attenuation

“…It was generally used to treat lung fever, cold blood, help reduce blood pressure, etc17. The crude extract of Cortex lycii radicis has growth inhibition effect on GBM cells (U87MG)16.…”