Dietary supplementation of pyrroloquinoline quinone disodium protects against oxidative stress and liver damage in laying hens fed an oxidized sunflower oil-added diet

Abstract

The protective effects of dietary pyrroloquinoline quinone disodium (PQQ.Na 2 ) supplementation against oxidized sunflower oil-induced oxidative stress and liver injury in laying hens were examined. Three hundred and sixty 53-week-old Hy-Line Gray laying hens were randomly allocated into one of the five dietary treatments. The treatments included: (1) a diet containing 2% fresh sunflower oil; (2) a diet containing 2% thermally oxidized sunflower oil; (3) an oxidized sunflower oil diet with 100 mg/kg of added vitamin E; (4) an oxidized sunflower oil diet with 0.08 mg/kg of PQQ.Na 2 ; and (5) an oxidized sunflower oil diet with 0.12 mg/kg of PQQ.Na 2 . Birds fed the oxidized sunflower oil diet showed a lower feed intake compared to birds fed the fresh oil diet or oxidized oil diet supplemented with vitamin E ( P = 0.009). Exposure to oxidized sunflower oil increased plasma malondialdehyde ( P < 0.001), hepatic reactive oxygen species ( P < 0.05) and carbonyl group levels ( P < 0.001), but decreased plasma glutathione levels (P = 0.006) in laying hens. These unfavorable changes induced by the oxidized sunflower oil diet were modulated by dietary vitamin E or PQQ.Na 2 supplementation to levels comparable to the fresh oil group. Dietary supplementation with PQQ.Na 2 or vitamin E increased the activities of total superoxide dismutase and glutathione peroxidase in plasma and the liver, when compared with the oxidized sunflower oil group ( P < 0.05). PQQ.Na 2 or vitamin E diminished the oxidized sunflower oil diet induced elevation of liver weight ( P = 0.026), liver to BW ratio ( P = 0.001) and plasma activities of alanine aminotransferase ( P = 0.001) and aspartate aminotransferase ( P < 0.001) and maintained these indices at the similar levels to the fresh oil diet. Furthermore, oxidized sunflower oil increased hepatic DNA tail length ( P < 0.05) and tail moment ( P < 0.05) compared with the fresh oil group. Dietary supplementation of PQQ.Na 2 or vitamin E decreased the oxidized oil diet induced DNA tail length and tail moment to the basal levels in fresh oil diet. These results indicate that PQQ.Na 2 is a potential antioxidant and is as effective against oxidized oil-related liver injury in laying hens as vitamin E. The protective effects of PQQ.Na 2 against liver damage induced by oxidized oil may be partially due to its role in the scavenging of free radicals, inhibiting of lipid peroxidation and enhancing of antioxidant defense systems.

Effect of pyrroloquinoline quinone disodium in female rats during gestating and lactating on reproductive performance and the intestinal barrier functions in the progeny

“…The use of PQQ in nutrition is increasingly being discussed in the literature ( 8 , 12 ) . There are several studies that have found beneficial effects of PQQ supplementation on the growth performance or body composition of growing rats ( 18 , 19 ) .…”

“…The potential number of catalyst cycles is approximately 2000, and four catalytic cycles of ascorbic acid have been reported ( 5 ) . Due to its antioxidant properties ( 11 ) , PQQ further exerts a neuroprotective and peritoneal protective action against mammalian oxidative stress ( 12 ) .…”

Effects of dietary quercetin on the antioxidative status and cecal microbiota in broiler chickens fed with oxidized oil

“…Lipid peroxidation not only damages DNA, protein, and membrane lipids but also decreases feed utilization and growth performance ( Liang et al., 2015 ). Broilers are easily exposed to cumulative oxidative by-products through oxidized oil intake, resulting in an imbalance of redox status especially in the liver, which is the target organ for detoxication ( Wang et al., 2016 ). Oxidized fish oil diet also caused microbial disorder and decreased the species richness and intestinal microbial diversity in fish ( Peng et al., 2019 ).…”

Effect of dietary pyrroloquinoline quinone disodium in sows on intestinal health of the offspring

“…The use of PQQ in nutrition is increasingly being discussed in the literature [13,21]. Zhang Unlike in humans, the weaning process for pigs involves an abrupt dietary shift from sow milk to a completely feed-based diet, which consequentially inhibits the intestinal architecture and function, resulting in gut associated disorders, such as in ammation and diarrhea [23].…”