Toxicological evaluation of an Allium-based commercial product in a 90-day feeding study in Sprague–Dawley rats

Mellado-García, Pilar; Puerto, María; Pichardo, Silvia; Llana-Ruiz-Cabello, María; Moyano, Rosario; Blanco, Alfonso; Jos, Ángeles; Cameán, Ana M.

doi:10.1016/j.fct.2016.01.019

Cited by 20 publications

(17 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Despite toxicological data of organosulfur from garlic are limited; in vitro studies report no mutagenicity (210). Besides, oral administration of allium extracts showed no mortality or side effects in rats (211). Importantly, the beneficial effect of these compounds is closely related to its bioavailability, and therefore thermal instability of these compounds must be considered (212).…”

Section: Garlic-derived Organosulfur Compoundsmentioning

confidence: 99%

Naturally Derived Heme-Oxygenase 1 Inducers and Their Therapeutic Application to Immune-Mediated Diseases

et al. 2020

View full text Add to dashboard Cite

Heme oxygenase (HO) is the primary antioxidant enzyme involved in heme group degradation. A variety of stimuli triggers the expression of the inducible HO-1 isoform, which is modulated by its substrate and cellular stressors. A major anti-inflammatory role has been assigned to the HO-1 activity. Therefore, in recent years HO-1 induction has been employed as an approach to treating several disorders displaying some immune alterations components, such as exacerbated inflammation or self-reactivity. Many natural compounds have shown to be effective inductors of HO-1 without cytotoxic effects; among them, most are chemicals present in plants used as food, flavoring, and medicine. Here we discuss some naturally derived compounds involved in HO-1 induction, their impact in the immune response modulation, and the beneficial effect in diverse autoimmune disorders. We conclude that the use of some compounds from natural sources able to induce HO-1 is an attractive lifestyle toward promoting human health. This review opens a new outlook on the investigation of naturally derived HO-1 inducers, mainly concerning autoimmunity.

show abstract

Section: Garlic-derived Organosulfur Compoundsmentioning

confidence: 99%

Naturally Derived Heme-Oxygenase 1 Inducers and Their Therapeutic Application to Immune-Mediated Diseases

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The interest in the genotoxicity evaluation of PTS is growing because of new potential applications in the food industry (feed additive, active in food packaging, etc.). Genotoxicity studies are one of the basic sets of toxicological studies included in the guidelines on the safety of feed additives, or substances employed in food contact materials, required by EFSA [ 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 ] before their approval.…”

Section: Discussionmentioning

confidence: 99%

“…Toxicological data about Allium extracts and essential oil are still scarce, especially in vivo. However, subchronic toxicity has been studied through a 90-day oral assay of Proallium [ 22 ], whose main component is PTSO. In this study, the extract demonstrated a complete absence of signs of toxicity.…”

Section: Introductionmentioning

confidence: 99%

Genotoxicity Evaluation of Propyl-Propane-Thiosulfinate (PTS) from Allium genus Essential Oils by a Combination of Micronucleus and Comet Assays in Rats

Cascajosa-Lira

Puerto

Prieto

et al. 2021

Foods

View full text Add to dashboard Cite

Propyl-propanethiosulfinate (PTS) is a component of Allium essential oils. This organosulfur molecule can be used as a feed additive to decrease the appearance of bacterial resistances caused by the residues of antibiotics. In previous in vitro genotoxicity studies, contradictory results were reported for PTS. In this work, the in vivo genotoxicity of PTS in male and female rats was assessed for the first time, following OECD (Organisation for Economic Co-operation and Development) guidelines. After oral administration (doses: 5.5, 17.4, and 55.0 mg/kg PTS body weight), a combination of the micronucleus (MN) assay (OECD 474) in bone marrow and the standard and enzyme-modified comet assay (OECD 489) was performed. After necropsy, histopathological studies were also carried out. The results did not show the in vivo genotoxicity of PTS at any doses assayed, revealed by the absence of increased MN, and DNA strand breaks or oxidative DNA damage in the standard and enzyme-modified comet assays. The histopathological study revealed that only the highest dose tested (55.0 mg/kg) in the liver and all dose groups in the stomach presented minimal pathological lesions in the organs studied. Consequently, the present work confirms that PTS is not genotoxic at the doses assayed, and it is a promising natural alternative to synthetic preservatives and antibiotics in animal feed.

show abstract

“…Therefore, the selected doses were calculated by dividing this data by a factor of 2, being the doses 50, 100 y 200 mg/kg b.w./day (d). Dietary dose individual formulations were prepared according to Mellado-García et al (2016).…”

Section: Dietsmentioning

confidence: 99%

A subchronic 90-day oral toxicity study of Origanum vulgare essential oil in rats

Llana-Ruiz-Cabello

Maisanaba

Puerto

et al. 2017

Food and Chemical Toxicology

Self Cite

View full text Add to dashboard Cite

Toxicological evaluation of an Allium-based commercial product in a 90-day feeding study in Sprague–Dawley rats

Cited by 20 publications

References 25 publications

Naturally Derived Heme-Oxygenase 1 Inducers and Their Therapeutic Application to Immune-Mediated Diseases

Naturally Derived Heme-Oxygenase 1 Inducers and Their Therapeutic Application to Immune-Mediated Diseases

Genotoxicity Evaluation of Propyl-Propane-Thiosulfinate (PTS) from Allium genus Essential Oils by a Combination of Micronucleus and Comet Assays in Rats

A subchronic 90-day oral toxicity study of Origanum vulgare essential oil in rats

Contact Info

Product

Resources

About