MMP2-sensing up-conversion nanoparticle for fluorescence biosensing in head and neck cancer cells

Chan, Yung Chieh; Chen, Chieh Wei; Chan, Ming Hsien; Chang, Yu Chan; Chang, Wei‐Min; Chi, Li-Hsing; Yu, Hui; Lin, Yu-Fung; Tsai, Din Ping; Liu, Ru‐Shi; Hsiao, Michael

doi:10.1016/j.bios.2016.01.049

Cited by 45 publications

(34 citation statements)

References 16 publications

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“…These reports suggest that MMP2 is regulated in highly dynamic manner at multiple levels. It has been reported that deregulation of MMP2 can lead to the cancerous growth of tissue and hence its activity has to be controlled in a growing tissue (Hojilla et al, ; Chan et al, ). Other than ECM remodelling during wound healing, the MMP2 localization result here in this study explains an indirect role for MMP2 in the proliferative blastema stage.…”

Section: Discussionmentioning

confidence: 99%

Variation in expression and activity pattern ofmmp2andmmp9on different time scales in the regenerating caudal fin ofPoecilia latipinna

Murawala

Patel

Ranadive

et al. 2018

Journal of Fish Biology

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Alteration in the expression pattern of matrix metalloproteinase (MMP)2 and MMP9 was studied in the regenerating caudal fin of Poecilia latipinna immediately following amputation until the new tissues gained structural integrity. Timed expression pattern of these two MMPs was studied at enzyme, transcript as well as protein levels. Additionally, both the gelatinases were localized in the regenerating caudal fin during three specific stages of regeneration. The results revealed a progressive increase in the expression of MMP2 starting at 1 h post amputation (hpa), indicating its possible role in the remodelling of extracellular matrix early on during caudal-fin regeneration. Nevertheless, a reduction in transcript level expression of MMP2 at 6 hpa and 12 hpa stages, points towards a possible transcriptional regulation, to further moderate its activity. As observed in the case of MMP2, expression of MMP9 too increased from 1 hpa and remained elevated until 5 dpa. However, the active MMP9 revealed its presence only 12 hpa onwards. Moreover, both the gelatinases were localised in the apical epithelial cap and in the progress zone at wound epithelium (1 dpa) and blastema (60 hpa) stages respectively. Further, during early differentiation stage (5 dpa), high intensities of MMP2 and MMP9 were localized in the newly formed actinotrichia as compared with the tissue proximal to it. Based on the results, it could be construed that the controlled up-regulation of MMP2 and MMP9 from 1 hpa until the early differentiation stage ensures a regulated digestion of extracellular matrix, perhaps to facilitate the recruitment, proliferation, morphogenesis and re-patterning of resident stem cells during caudal fin regeneration in P. latipinna.

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Section: Discussionmentioning

confidence: 99%

Variation in expression and activity pattern ofmmp2andmmp9on different time scales in the regenerating caudal fin ofPoecilia latipinna

Murawala

Patel

Ranadive

et al. 2018

Journal of Fish Biology

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“…Moreover, UCNP@p-QD showed the same consequence in OEC-M1 and FADU cells (Figure S5, Supporting Information ), which were MMP2-null and MMP2-overexpressing cells, respectively. 35 This result indicated that UCNP@p-QD could be detected in extracellular MMP2. The effective penetration is usually an obstacle for scientists to develop the photodependent biosensors, so the high penetrating infrared has attracted the attention of investigators.…”

Section: Results and Discussionmentioning

confidence: 83%

“… 23 UCNP@p was conjugated with the CIS/ZnS QD in DMSO/chloroform solution (1:5) for 24 h conjugation because of disulfide binding. 35 The folic acid (10 nM) was further conjugated onto UCNP@p-QD using the NHS/EDC method. The upconversion nanoparticles were coated with silica shed using a modified microemulsion method.…”

Section: Methodsmentioning

confidence: 99%

Near-Infrared-Activated Fluorescence Resonance Energy Transfer-Based Nanocomposite to Sense MMP2-Overexpressing Oral Cancer Cells

Chan

Chen

et al. 2018

ACS Omega

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The matrix metalloproteinases (MMPs) are well-known mediators that are activated in tumor progression. MMP2 is a kind of gelatinase in extracellular matrix remodeling and cancer metastasis processes. MMP2 secretion increased in many types of cancer diseases, and its abnormal expression is associated with a poor prognosis. We fabricated a nanocomposite that sensed MMP2 expression by a red and blue light change. This nanocomposite consisted of an upconversion nanoparticle (UCNP), MMP2-sensitive peptide, and CuInS2/ZnS quantum dot (CIS/ZnS QD). An UCNP is composed of NaYF4:Tm/Yb@NaYF4:Nd/Yb, which has multiple emissions at UV/blue-visible wavelengths under 808 nm laser excitation. The conjugated CIS/ZnS QD showed the red-visible fluorescence though the FRET process. The two fluorophores were connected by a MMP2-sensitive peptide to form a novel MMP2 biosensor, named UCNP@p-QD. UCNP@p-QD was highly biocompatible according to cell viability assay. The FRET-based biosensor was employed in the MMP2 determination in vitro and in vivo. Furthermore, it was administrated into the tumor-bearing mouse to check MMP2 expression. UCNP@p-QD could be a promising tool for biological study and biomedical application. In this study, we demonstrated that the CIS/ZnS QD improved the upconversion intensity through a near-infrared-induced FRET process. This nanocomposite has the advantage of light penetration, excellent biocompatibility, and high sensitivity to sense MMP2. The near-infrared-induced composites are a potential inspiration for use in biomedical applications.

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“…For instance, a complex of UCNPs with GNPs targeting MMP2-recognizing peptides was employed for HN cancer-cell visualization in vitro. 74 For improved properties, UCNPs can be surrounded by other structures, such as erythrocyte membrane.…”

Section: And 111mentioning

confidence: 99%

Future trends and emerging issues for nanodelivery systems in oral and oropharyngeal cancer

Irimie

Sonea²,

Jurj

et al. 2017

IJN

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Oral cancer is a prevalent cancer type on a global scale, whose traditional treatment strategies have several drawbacks that could in the near future be overcome through the development of novel therapeutic and prognostic strategies. Nanotechnology provides an alternative to traditional therapy that leads to enhanced efficiency and less toxicity. Various nanosystems have been developed for the treatment of oral cancer, including polymeric, metallic, and lipid-based formulations that incorporate chemotherapeutics, natural compounds, siRNA, or other molecules. This review summarizes the main benefits of using these nanosystems, in parallel with a particular focus on the issues encountered in medical practice. These novel strategies have provided encouraging results in both in vitro and in vivo studies, but few have entered clinical trials. The use of nanosystems in oral cancer has the potential of becoming a valid therapeutic option for patients suffering from this malignancy, considering that clinical trials have already been completed and others are currently being developed.

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MMP2-sensing up-conversion nanoparticle for fluorescence biosensing in head and neck cancer cells

Cited by 45 publications

References 16 publications

Variation in expression and activity pattern ofmmp2andmmp9on different time scales in the regenerating caudal fin ofPoecilia latipinna

Variation in expression and activity pattern ofmmp2andmmp9on different time scales in the regenerating caudal fin ofPoecilia latipinna

Near-Infrared-Activated Fluorescence Resonance Energy Transfer-Based Nanocomposite to Sense MMP2-Overexpressing Oral Cancer Cells

Future trends and emerging issues for nanodelivery systems in oral and oropharyngeal cancer

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