Tumor suppressor role of microRNA-1296 in triple-negative breast cancer

Phan, Binh; Majid, Shahana; Ursu, Sarah; Semir, David de; Nosrati, Mehdi; Bezrookove, Vladimir; Kashani‐Sabet, Mohammed; Dar, Altaf A.

doi:10.18632/oncotarget.6961

Cited by 45 publications

(56 citation statements)

References 38 publications

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“…MiRNA-next generation sequencing analysis reveals the potential association of miR-1296 with breast cancer chemoresistance and self-renewal capability [4]. The other studies show that miR-1296 is down-regulated in prostate cancer and triple negative breast cancer (TNBC) samples [5, 6], suggesting that it might have a role as a tumor-suppressor gene in these cancers mentioned above. However, the role of miR-1296-5p as tumor-suppressor in cancer metastasis is still largely unknown.…”

Section: Discussionmentioning

confidence: 99%

“…On cellular and molecular levels, miR-1296 overexpression plays a evidently suppressive role in the expression of Cyclin D1 and the multiplying of TNBC cell lines [5]. While in prostate cancer PC3 cells of human beings, the low expression of miR-1296 can upregulate both minichromosome maintenance 2 (MCM2) mRNA and protein, and conversely, its overexpression can lower the level of the both and even shorten the S phase of the cell cycle [6].…”

Section: Discussionmentioning

confidence: 99%

“…For instance, miR-1296 has been proved of its potential linkage with breast cancer chemoresistance and self-renewal capability [4]. In triple negative breast cancer (TNBC) cell lines and tissues samples, miR-1296 expression was significantly suppressed and increased the sensitiveness of TNBC cells to cisplatin treatment [5]. MiR-1296 was found significantly downregulated in prostate cancer tissues, bringing about an obvious decrease in the S phase of the cell cycle [6].…”

Section: Introductionmentioning

confidence: 99%

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miR 1296-5p Inhibits the Migration and Invasion of Gastric Cancer Cells by Repressing ERBB2 Expression

et al. 2017

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The metastasis of gastric cancer, one of the most common tumors, has a molecular mechanism that is still largely unclear. Here we investigated the role of possible tumor-suppressor miR-1296-5p in the cell migration and invasion of ERBB2-positive gastric cancer. It found that miR-1296-5p was significantly down-regulated in gastric cancer tissues. Moreover, it was down-regulated in lymph node metastatic gastric cancer tissues compared with non-metastatic gastric cancer tissues. The luciferase activity of ERBB2 3'-untranslated region-based reporters constructed in SNU-216 and NUGC-4 gastric cancer cells suggested that ERBB2 was the target gene of miR-1296-5p. Overexpressed miR-1296-5p reduced its target protein level and Rac1 activation, and inhibited the migration and invasion of SNU-216 and NUGC-4 gastric cancer cells. MiR-1296-5p was down-regulated in ERBB2-positive gastric cancer tissues compared with ERBB2-negative gastric cancer tissues. In ERBB2-positive gastric cancers, the miR-1296-5p expression was suppressed in a majority of metastatic lymph node tissues compared to non-metastatic gastric cancer samples. The migration and invasion of gastric cancer cells was inhibited by miR-1296-5p overexpression or herceptin treatment, and rescued by the overexpression of constitutively active Rac1-Q61L or ERBB2. Taken together, our findings first suggest that miR-1296-5p might be involved in the regulation on the migration and invasion of human gastric cancer cells at least in part via targeting ERBB2/Rac1 signaling pathway.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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miR 1296-5p Inhibits the Migration and Invasion of Gastric Cancer Cells by Repressing ERBB2 Expression

et al. 2017

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“…miR-147, miR-340 and miR-1296) in breast cancer tissues or cells, and revealed their potential roles in breast cancer progression by contributing to cell proliferation, survival and metastasis. 17, 18, 19 However, the molecular basis for these inhibitory effects of miR-26a/26b targeting ST8SIA4 has not been fully elucidated yet in breast cancer.…”

mentioning

confidence: 99%

Functional roles of sialylation in breast cancer progression through miR-26a/26b targeting ST8SIA4

Dong

et al. 2016

Cell Death Dis

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Sialylation is one of the altered glycosylation patterns associated with cancer progression. In this study, we investigated the N-glycan profiles of breast cancer patients and cell lines to reveal sialylation associated with breast cancer progression, and provided new evidences of miRNA-mediated sialylation. MALDI-TOF MS analysis revealed that N-glycans found in breast cancer tissues and breast cancer cell MDA-MB-231 featured increased levels of sialylation compared with adjacent tissues and normal breast epithelial cell MCF-10A. The expressional profiles of 20 sialyltransferase genes were then analyzed and found significantly different comparing breast cancer samples with adjacent tissues, and two breast cancer cell lines MDA-MB-231 and MCF-7 with different metastatic potential and MCF-10A cells. Tumor tissues and highly metastatic breast cancer cell line MDA-MB-231 exhibited higher levels of ST8SIA4. Knocking down ST8SIA4 in breast cancer cell lines significantly inhibited their malignant behaviors including cell proliferation and invasion in a sialyltransferase-dependent manner. By applying bioinformatic approaches for the prediction of miRNA targeting 3′-UTR of ST8SIA4, we identified ST8SIA4 as one of the miR-26a/26b-targeted genes. Further data analysis revealed the inversely related expression of ST8SIA4 and miR-26a/26b in breast cancer cells, tumor tissues and corresponding adjacent tissues. The ability of miR-26a/26b to interact specifically with and regulate the 3′-UTR of ST8SIA4 was demonstrated via a luciferase reporter assay. The forced expression of miR-26a/26b was able to induce a decrease of ST8SIA4 level and also to affect breast cancer cells progression, while altered expression of ST8SIA4 in breast cancer cells modulated progression upon transfection with miR-26a/26b mimics or inhibiter. Taken together, these results indicate that changes in the glycosylation patterns and sialylation levels may be useful markers of the progression of breast cancer, as well as miR-26a/26b may be widely involved in the regulation of sialylation machinery by targeting ST8SIA4.

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“…As above, miR-1296 is associated with cancer and metastasis, with one study finding it down-regulated in gastric cancer (compared with adjacent normal tissue) and even more down-regulated in lymph node metastases (compared with gastric cancer) [34]. Meanwhile, induction of miR-1296 was found to induce apoptosis in triple negative breast cancer [28]. Although the other miRNAs presented in Figure 5 have not yet been associated with metastasis per se, several have been linked to cancer more generally, including miR-423 which is found to promote tumour progression in laryngeal cancer [15], and miR-101-2 and miR-4687 which are found to be associated with gastric cancer [31] and breast cancer [25] respectively.…”

Section: Microrna As a Predictor Of Mrna Expressionmentioning

confidence: 93%

A cross-cancer metastasis signature in the microRNA-mRNA axis of paired tissue samples

Lee

Nguyen

Venkatesh

et al. 2018

Preprint

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In the progression of cancer, cells acquire genetic mutations that cause uncontrolled growth. Over time, the primary tumour may undergo additional mutations that allow for the cancerous cells to spread throughout the body as metastases. Since metastatic development typically results in markedly worse patient outcomes, research into the identity and function of metastasisassociated biomarkers could eventually translate into clinical diagnostics or novel therapeutics. Although the general processes underpinning metastatic progression are understood, no consistent nor clear cross-cancer biomarker profile has yet emerged. However, the literature suggests that some microRNAs (miRNAs) may play an important role in the metastatic progression of several cancer types. Using a subset of The Cancer Genome Atlas (TCGA) data, we performed an integrated analysis of mRNA and miRNA expression with paired metastatic and primary tumour samples to interrogate how the miRNA-mRNA regulatory axis influences metastatic progression. From this, we successfully built mRNA-and miRNA-specific classifiers that can discriminate pairs of metastatic and primary samples across 11 cancer types. In addition, we identified a number of miRNAs whose metastasis-associated dysregulation could predict mRNA metastasis-associated dysregulation. Among the most predictive miRNAs, we found several previously implicated in cancer progression, including miR-301b, miR-1296, and miR-423. Taken together, our results suggest that cross-cancer metastatic samples have unique biomarker signatures when compared with paired primary tumours, and that these miRNA biomarkers can be used to predict both metastatic status and mRNA expression.

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Tumor suppressor role of microRNA-1296 in triple-negative breast cancer

Cited by 45 publications

References 38 publications

miR 1296-5p Inhibits the Migration and Invasion of Gastric Cancer Cells by Repressing ERBB2 Expression

miR 1296-5p Inhibits the Migration and Invasion of Gastric Cancer Cells by Repressing ERBB2 Expression

Functional roles of sialylation in breast cancer progression through miR-26a/26b targeting ST8SIA4

A cross-cancer metastasis signature in the microRNA-mRNA axis of paired tissue samples

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