Nuclear ribosomal internal transcribed spacer 1 (ITS1) variation in the Anastrepha fraterculus cryptic species complex (Diptera, Tephritidae) of the Andean region

Sutton, Bruce D.; Steck, Gary J.; Norrbom, Allen L.; Rodríguez, Erick J.; Srivastava, Pratibha; Alvarado, Norma Nolazco; Colque, Fredy; Yábar-Landa, Erick; Quisberth, Elizabeth; Peñaranda, E. Arévalo; Clavijo, Pedro A. Rodriguez; Alvarez-Baca, Jeniffer K.; Zapata, Tito Guevara; Ponce, Patricio

doi:10.3897/zookeys.540.6147

Cited by 24 publications

(37 citation statements)

References 11 publications

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“…The polytene chromosome map for one morphotype was constructed (Gariou-Papalexiou et al unpublished data). (ITS 1)Internal Transcriber Spacer 1 was found to be a good molecular marker to identify different groups (Sutton et al 2015). Microsatellites were developed (Lanzavecchia et al 2014) and proved to be successful to discriminate among morphotypes (Lima et al unpublished data, Manni et al 2015).…”

Section: Situation Analysis At the Start Of The Crp And Ouputs For Eamentioning

confidence: 99%

Resolving cryptic species complexes of major tephritid pests

Hendrichs¹,

Vera²,

Meyer³

et al. 2015

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An (CRP)FAO/IAEA Co-ordinated Research Project on “Resolution of Cryptic Species Complexes of Tephritid Pests to Overcome Constraints to SIT Application and International Trade” was conducted from 2010 to 2015. As captured in the CRP title, the objective was to undertake targeted research into the systematics and diagnostics of taxonomically challenging fruit fly groups of economic importance. The scientific output was the accurate alignment of biological species with taxonomic names; which led to the applied outcome of assisting FAO and IAEA Member States in overcoming technical constraints to the application of the (SIT)Sterile Insect Technique against pest fruit flies and the facilitation of international agricultural trade. Close to 50 researchers from over 20 countries participated in the CRP, using coordinated, multidisciplinary research to address, within an integrative taxonomic framework, cryptic species complexes of major tephritid pests. The following progress was made for the four complexes selected and studied:Anastrepha fraterculus complex – Eight morphotypes and their geographic and ecological distributions in Latin America were defined. The morphotypes can be considered as distinct biological species on the basis of differences in karyotype, sexual incompatibility, post-mating isolation, cuticular hydrocarbon, pheromone, and molecular analyses. Discriminative taxonomic tools using linear and geometric morphometrics of both adult and larval morphology were developed for this complex.Bactrocera dorsalis complex – Based on genetic, cytogenetic, pheromonal, morphometric, and behavioural data, which showed no or only minor variation between the Asian/African pest fruit flies Bactrocera dorsalis, Bactrocera papayae, Bactrocera philippinensis and Bactrocera invadens, the latter three species were synonymized with Bactrocera dorsalis. Of the five target pest taxa studied, only Bactrocera dorsalis and Bactrocera carambolae remain as scientifically valid names. Molecular and pheromone markers are now available to distinguish Bactrocera dorsalis from Bactrocera carambolae.Ceratitis FAR Complex (Ceratitis fasciventris, Ceratitis anonae, Ceratitis rosa) – Morphology, morphometry, genetic, genomic, pheromone, cuticular hydrocarbon, ecology, behaviour, and developmental physiology data provide evidence for the existence of five different entities within this fruit fly complex from the African region. These are currently recognised as Ceratitis anonae, Ceratitis fasciventris (F1 and F2), Ceratitis rosa and a new species related to Ceratitis rosa (R2). The biological limits within Ceratitis fasciventris (i.e. F1 and F2) are not fully resolved. Microsatellites markers and morphological identification tools for the adult males of the five different FAR entities were developed based on male leg structures.Zeugodacus cucurbitae (formerly Bactrocera (Zeugodacus) cucurbitae) – Genetic variability was studied among melon fly populations throughout its geographic range in Africa and the Asia/Pacific region and found to ...

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Section: Situation Analysis At the Start Of The Crp And Ouputs For Eamentioning

confidence: 99%

Resolving cryptic species complexes of major tephritid pests

Hendrichs¹,

Vera²,

Meyer³

et al. 2015

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“…Hernández-Ortiz et al (2015) compared the same populations used in our study with other populations from Central America and northern South America, using linear and geometric morphology, and found that all of them belong to the Andean morphotype of the complex. However, genetic tools used by Sutton et al (2015) showed variability in Colombian specimens belonging to the Venezuelan or Peruvian morphotypes (those collected at low altitudes) and to the Andean morphotype (those from medium to high altitude); 4 specimens in the Andean morphotype showed a small polymorphism.…”

Section: Egg Size Variabilitymentioning

confidence: 99%

Phenotypic Structure of Colombian Populations ofAnastrepha fraterculusComplex (Diptera: Tephritidae)

Canal

Galeano-Olaya

Castañeda

2018

Florida Entomologist

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“…Recent studies focused on the elucidation of species from the A. fraterculus complex have followed an integrative approach. These scientific works addressed this taxonomic issue using different strategies based on morphology [73,77], behavior and reproductive isolation [76,[78][79][80][81], and cytology and genetics [82][83][84][85][86]. Based on mating compatibility studies [87][88][89] and population genetic analysis [90,91], a single biological entity of the A. fraterculus complex was identified in Argentina and southern Brazil.…”

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Wolbachiainfection in Argentinean populations ofAnastrepha fraterculus sp1: preliminary evidence of sex ratio distortion by one of two strains

Conte

Segura

Milla

et al. 2019

Preprint

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Background: Wolbachia, one of the most abundant taxa of intracellular Alphaproteobacteria, is widespread among arthropods and filarial nematodes. The presence of these maternally inherited bacteria is associated with modifications of host fitness, including a variety of reproductive abnormalities, such as cytoplasmic incompatibility, thelytokous parthenogenesis, host feminization and male-killing. Wolbachia has attracted much interest for its role in biological, ecological and evolutionary processes as well as for its potential use in novel and environmentally-friendly strategies for the control of insect pests and disease vectors including a major agricultural pest, the South American fruit fly, Anastrepha fraterculus Wiedemann (Diptera: Tephritidae). Results:We used wsp, 16S rRNA and a multilocus sequence typing (MLST) scheme including gatB, coxA, hcpA, fbpA, and ftsZ genes to detect and characterize the Wolbachia infection in laboratory strains and wild populations of A. fraterculus from Argentina. Wolbachia was found in all A. fraterculus individuals studied. Nucleotide sequences analysis of wsp gene allowed the identification of two Wolbachia nucleotide variants (named wAfraCast1_A and wAfraCast2_A). After the analysis of 76 individuals, a high prevalence of the wAfraCast2_A variant was found both, in laboratory (82%) and wild populations (95%). MLST analysis identified both Wolbachia genetic variants as sequence type 13. Phylogenetic analysis of concatenated MLST datasets clustered wAfraCast1/2_A in the supergroup A. Paired-crossing experiments among single infected laboratory strains showed a phenotype specifically associated to wAfraCast1_A that includes slight detrimental effects on larval survival, a female-biased sex ratio; suggesting the induction of male-killing phenomena, and a decreased proportion of females producing descendants that appears attributable to the lack of sperm in their spermathecae. Conclusions:We detected and characterized at the molecular level two wsp gene sequence variants of Wolbachia both in laboratory and wild populations of A. fraterculus sp.1 from Argentina. Crossing experiments on singly-infected A. fraterculus strains showed evidence of a male killing-like mechanism potentially associated to the wAfraCast1_A -A. fraterculus interactions. Further mating experiments including antibiotic treatments and the analysis of early and late immature stages of descendants will contribute to our understanding of the phenotypes elicited by the Wolbachia variant wAfraCast1_A in A. fraterculus sp.1.

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Nuclear ribosomal internal transcribed spacer 1 (ITS1) variation in the Anastrepha fraterculus cryptic species complex (Diptera, Tephritidae) of the Andean region

Cited by 24 publications

References 11 publications

Resolving cryptic species complexes of major tephritid pests

Resolving cryptic species complexes of major tephritid pests

Phenotypic Structure of Colombian Populations ofAnastrepha fraterculusComplex (Diptera: Tephritidae)

Wolbachiainfection in Argentinean populations ofAnastrepha fraterculus sp1: preliminary evidence of sex ratio distortion by one of two strains

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