Isolation, Characterization, and Differentiation of Dental Pulp Stem Cells in Ferrets

Homayounfar, Negar; Verma, Prashant; Nosrat, Ali; Ayachi, Ikbale El; Yu, Zongdong; Romberg, Elaine; Huang, George T.‐J.; Fouad, Ashraf F.

doi:10.1016/j.joen.2015.12.002

Cited by 18 publications

(15 citation statements)

References 36 publications

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“…Cells were passaged at the ratio of 1:3 when they reached 85-90 % confluence. The cell populations were characterized by positive staining with anti-CD90 (Sigma-Aldrich, St. Louis, MO, USA), anti-CD105 (Sigma-Aldrich, USA), anti-CD146 (Sigma-Aldrich) and STRO-1 (Sigma-Aldrich) and the absence of CD45 (Sigma-Aldrich) (Homayounfar et al 2016). Cells at passage 3 (P 3 ) were used in the subsequent experiments.…”

Section: Cell Culturesmentioning

confidence: 99%

The role of oncostatin M regulates osteoblastic differentiation of dental pulp stem cells through STAT3 pathway

et al. 2016

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Dental pulp stem cells (DPSCs) are a type of mesenchymal stem cells, which have the selfrenewal and multi-lineage differentiation potential, including chondrocytes, adipocytes, neural cells and osteoblasts. So they play a significant role in pulp repair and bone regeneration. Oncostatin M (OSM), one of the IL-6 family cytokines, inhibits adipogenic differentiation and stimulates osteogenic differentiation of human bone marrow mesenchymal stem cells. However, the effect of OSM on DPSCs is unclear. We found that OSM induced osteogenic differentiation of DPSCs, promoting matrix mineralization as measured by Alizarin Red S staining. OSM also increased expression of osteogenesis-associated gene products Alkaline phosphatase, Bone morphogenetic protein 2 (BMP2), Runt-related transcription factor 2 and Osteocalcin (OCN) as assessed by immunoblotting. We also found that OSM activated the Signal Transducer And Activator Of Transcription 3 (STAT3) pathway during the osteogenic differentiation of DPSCs. Blocking the osteogenic differentiation by silencing of STAT3 can significantly inhibit OSMinduced osteogenic differentiation of DPSCs and the expression of related genes, furthermore matrix mineralization was also suppressed. In summary, OSM promotes osteoblastic differentiation of DPSCs and osteogenesis-related genes expression through the JAK3/STAT3 signaling pathway which may be useful for the autologous transplantation of DPSCs.

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Section: Cell Culturesmentioning

confidence: 99%

The role of oncostatin M regulates osteoblastic differentiation of dental pulp stem cells through STAT3 pathway

et al. 2016

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“…However, extended in vitro culture using current methodologies is known to cause loss of hDPCs' regenerative capacity . Stro1 is an early marker of stem cells, and its expression decreased at later passages . Oct4 and Sox2 levels in human embryonic stem cells (hESCs) characterize the undifferentiated state, and loss expression of Oct4 and Sox2 resulted in cell differentiation and progressive loss of pluripotency .…”

Section: Discussionmentioning

confidence: 99%

“…26 Stro1 is an early marker of stem cells, and its expression decreased at later passages. 27 Oct4 and Sox2 levels in human embryonic stem cells (hESCs) characterize the undifferentiated state, and loss expression of Oct4 and Sox2 resulted in cell differentiation and progressive loss of pluripotency. 28 Recent studies have reported that hDPCs can be rejuvenated by optimizing the extracellular microenvironment, which regulates pluripotency-related genes such as Stro1, Oct4, and Sox2 to modulate cells' regenerative properties.…”

Section: Discussionmentioning

confidence: 99%

The extracts of bredigite bioceramics enhanced the pluripotency of human dental pulp cells

Liu

Yang

et al. 2017

J Biomedical Materials Res

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Biomaterials have a profound effect on tissue engineering and regenerative medicine, but few studies have reported the role of extracts from bioceramics in the regulation of stem cell pluripotency. The present study investigated the effects of bioceramics extracts, including silicate bredigite (Ca7MgSi4O16) and conventional β‐tricalcium phosphate (β‐TCP), on the pluripotency and the multilineage differentiation potential of human dental pulp cells (hDPCs). Basic fibroblast growth factor (bFGF), which is a known regulator of hDPCs pluripotency, was used as a reference. Bredigite extracts significantly promoted cell growth, proliferation, TERT expression and maintained hDPCs in a presenescent state. The extracts of bredigite significantly up‐regulated the expression of pluripotency‐related genes such as Stro1, Oct4 and Sox2, and further promoted the multilineage differentiation of hDPCs after odontogenic/adipogenic induction. The stimulation of bredigite extracts on hDPCs pluripotency was comparable to that of bFGF, whereas β‐TCP extracts lacked these properties. Our results suggested for the first time that bredigite extracts enhance the pluripotency of dental‐derived stem cells, paving the way for extended applications in regenerative medicine. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 3465–3474, 2017.

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“…An earlier report using the ferret model for regenerative procedures showed that the use of blood clot or platelet-rich plasma resulted in the formation of mineralized tissue that mimics osseous or cemento-osseous tissues. 36 Recent studies have isolated and characterized DPSCs from the ferret canine (fDPSCs), 37 which were found to express STRO1, CD90, and CD105, but not CD146, and were highly osteogenic but weakly adipogenic. Quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) experiments revealed that the cells expressed osteopontin, DMP1, DSP, and alkaline phosphatase (ALP) comparable with their human counterparts.…”

Section: Ferret Model (Mustela Poturios Furo)mentioning

confidence: 99%

Animal Models for Stem Cell-Based Pulp Regeneration: Foundation for Human Clinical Applications

Nakashima

Iohara

Bottino

et al. 2019

Tissue Engineering Part B: Reviews

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Rapid progress has been made in the last decade related to stem cell-mediated pulp-dentin regeneration, from characterization of dental pulp stem cells (DPSCs) to the first-ever reported clinical case in humans. However, many challenges still need to be addressed before such technology can become a common clinical practice; therefore, further rigorous research is needed. Animal study models are very important to test new ideas, concepts, and technologies. This review summarizes and discusses several key animal models that have been utilized to investigate pulp-dentin regeneration. From a tissue regeneration perspective, we categorize the animal model by the location where the regenerated pulp tissue is formed: ectopic, semiorthotopic, and orthotopic. Several animal species are discussed, including mouse, ferret, dog, and miniswine. Mouse is used for ectopic pulp-dentin regeneration in the dorsum subcutaneous space. A commonly tested approach is hydroxyapatite/tricalcium phosphate (HA-TCP) granules model used to observe ectopic pulp-dentin complex formation. The semiorthotopic model includes tooth slices or fragments with which de novo pulp regeneration in a root canal space can be tested in the mouse subcutaneous space. For orthotopic pulp regeneration, the canine teeth of ferrets are large enough for such purposes. As nonprimate large animal models, dog and miniswine teeth have many aspects quite similar to those of humans, allowing researchers to perform experiments that mimic clinical conditions in humans. The protocols established and the data obtained from large animal studies may directly relate to and apply to future human studies. Complete orthotopic pulp regeneration has been demonstrated in dogs and miniswine. The use of allogeneic and subpopulations of DPSCs for pulp regeneration, and testing of the periapical disease model and aging model have been performed in miniswine or dogs. In sum, all these animal models will help address challenges that still face pulp regeneration in humans. We need to thoroughly utilize these models to test new ideas, technologies, and strategies before reliable and predictable clinical protocols can be established for human clinical trials or treatment.

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Isolation, Characterization, and Differentiation of Dental Pulp Stem Cells in Ferrets

Cited by 18 publications

References 36 publications

The role of oncostatin M regulates osteoblastic differentiation of dental pulp stem cells through STAT3 pathway

The role of oncostatin M regulates osteoblastic differentiation of dental pulp stem cells through STAT3 pathway

The extracts of bredigite bioceramics enhanced the pluripotency of human dental pulp cells

Animal Models for Stem Cell-Based Pulp Regeneration: Foundation for Human Clinical Applications

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