The level and distribution pattern of HP1β in the embryonic brain correspond to those of H3K9me1/me2 but not of H3K9me3

Bártová, Eva; Večeřa, Josef; Krejčí, Jana; Legartová, Soňa; Pachernı́k, Jiřı́; Kozubek, Stanislav

doi:10.1007/s00418-015-1402-7

Cited by 8 publications

(8 citation statements)

References 41 publications

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“…For analysis, we used the "tile-scanning" mode, a toll of Leica SP-5 confocal microscope (Leica, Germany). To acquire images, we used HCX PL APO lambda blue 20.0× 0.7 IMM UV objective (Leica Microsystems, Germany) (see Bartova et al 2016).…”

Section: The Tile-scanningmentioning

confidence: 99%

Cell differentiation and aging is accompanied by depletion of the ACE2 protein

Bártová

Legartová

Krejčí

et al. 2020

Preprint

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Angiotensin-converting enzyme (ACE) is a zinc metalloproteinase involved in the renin-angiotensin system (RAS). It is well known that ACE and ACE2 are central regulators of blood pressure. Moreover, recently, it was observed that the ACE2 protein is the main target of the SARS-CoV-2 virus, so we have tried to reveal if there is a distinction in the levels of the ACE2 protein in distinct cell types (sensitive to virus infection), during cell differentiation and aging. We observed that depletion of the ACE2 protein appears in aorta-associated parts during the aging of adult mice, and the level of ACE2 was lowest in kidneys of old female animals in comparison to male mice. Differentiation into enterocytes and more pronouncedly into cardiomyocytes was accompanied by depletion of the ACE2 protein. The deficiency of histone deacetylase 1 (HDAC1) also caused a decrease in the level of both ACE2 and its interacting partner renin. However, experimental cardiomyogenesis was associated with renin up-regulation. In human lung adenocarcinoma cells, vitamin D2, but not chloroquine, slightly increased the level of ACE2. Together, the higher level of the ACE2 protein appears in non-differentiated cells and tissue of young mice, in comparisons to terminally differentiated cells and old animals; thus, a higher level of the ACE2 protein, also seen after vitamin D2 treatment, seems to be a barrier against SARS-CoV-2, because it is known that tissues of young individuals are less sensitive to viral infection.

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Section: The Tile-scanningmentioning

confidence: 99%

Cell differentiation and aging is accompanied by depletion of the ACE2 protein

Bártová

Legartová

Krejčí

et al. 2020

Preprint

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“…Neuronal cells were prepared according to Bartova et al ., 2016 . Neural stem/progenitors cells (NSCs) were isolated from the embryonic ganglionic eminence (GE) of the forebrain of C57/BL6 mice at 13.5 dpc.…”

Section: Methodsmentioning

confidence: 99%

Selective elimination of neuroblastoma cells by synergistic effect of Akt kinase inhibitor and tetrathiomolybdate

Navrátilová

Karasová

Lánová

et al. 2017

J Cellular Molecular Medi

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Neuroblastoma is the most common extracranial solid tumour of infancy. Pathological activation of glucose consumption, glycolysis and glycolysis‐activating Akt kinase occur frequently in neuroblastoma cells, and these changes correlate with poor prognosis of patients. Therefore, several inhibitors of glucose utilization and the Akt kinase activity are in preclinical trials as potential anti‐cancer drugs. However, metabolic plasticity of cancer cells might undermine efficacy of this approach. In this work, we identified oxidative phosphorylation as compensatory mechanism preserving viability of neuroblastoma cells with inhibited glucose uptake/Akt kinase. It was oxidative phosphorylation that maintained intracellular level of ATP and proliferative capacity of these cells. The oxidative phosphorylation inhibitors (rotenone, tetrathiomolybdate) synergized with inhibitor of the Akt kinase/glucose uptake in down‐regulation of both viability of neuroblastoma cells and clonogenic potential of cells forming neuroblastoma spheroids. Interestingly, tetrathiomolybdate acted as highly specific inhibitor of oxygen consumption and activator of lactate production in neuroblastoma cells, but not in normal fibroblasts and neuronal cells. Moreover, the reducing effect of tetrathiomolybdate on cell viability and the level of ATP in the cells with inhibited Akt kinase/glucose uptake was also selective for neuroblastoma cells. Therefore, efficient elimination of neuroblastoma cells requires inhibition of both glucose uptake/Akt kinase and oxidative phosphorylation activities. The use of tetrathiomolybdate as a mitochondrial inhibitor contributes to selectivity of this combined treatment, preferentially targeting neuroblastoma cells.

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“…The tissues were permeabilized in 1% Triton X-100 and 0.1% saponin (Sigma-Aldrich, Czech Republic) dissolved in PBS. Immunohistochemistry was performed according to the protocols described by Bártová et al [49]. In our studies, we used a primary antibody raised against coilin (H-300) (#sc-32860, Santa Cruz, USA), fibrillarin (#ab4566, Abcam, UK), and a goat anti-rabbit Alexa Fluor 594 secondary antibody (#A11012, Invitrogen) or goat anti-mouse Alexa Fluor 594 (#A11032, Invitrogen, USA) or anti-rabbit Alexa Fluor 488 (#ab150077, Abcam, UK).…”

Section: Methodsmentioning

confidence: 99%

“…For 3D projections, we obtained 30–40 optical sections with axial steps of 0.3 μ m. 3D projection reconstruction was conducted using the Leica Application Suite (LAS) software. The scanning of larger biological objects, such as embryonic brain sections, was conducted in tile scanning mode with the Leica software, as previously described [49]. …”

Section: Methodsmentioning

confidence: 99%

Neural Differentiation in HDAC1-Depleted Cells Is Accompanied by Coilin Downregulation and the Accumulation of Cajal Bodies in Nucleoli

Krejčí

Legartová

Bártová

2017

Stem Cells International

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Cajal bodies (CBs) are important compartments containing accumulated proteins that preferentially regulate RNA-related nuclear events, including splicing. Here, we studied the nuclear distribution pattern of CBs in neurogenesis. In adult brains, coilin was present at a high density, but CB formation was absent in the nuclei of the choroid plexus of the lateral ventricles. Cells of the adult hippocampus were characterized by a crescent-like morphology of coilin protein. We additionally observed a 70 kDa splice variant of coilin in adult mouse brains, which was different to embryonic brains and mouse pluripotent embryonic stem cells (mESCs), characterized by the 80 kDa standard variant of coilin. Here, we also showed that depletion of coilin is induced during neural differentiation and HDAC1 deficiency in mESCs caused coilin accumulation inside the fibrillarin-positive region of the nucleoli. A similar distribution pattern was observed in adult brain hippocampi, characterized by lower levels of both coilin and HDAC1. In summary, we observed that neural differentiation and HDAC1 deficiency lead to coilin depletion and coilin accumulation in body-like structures inside the nucleoli.

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The level and distribution pattern of HP1β in the embryonic brain correspond to those of H3K9me1/me2 but not of H3K9me3

Cited by 8 publications

References 41 publications

Cell differentiation and aging is accompanied by depletion of the ACE2 protein

Cell differentiation and aging is accompanied by depletion of the ACE2 protein

Selective elimination of neuroblastoma cells by synergistic effect of Akt kinase inhibitor and tetrathiomolybdate

Neural Differentiation in HDAC1-Depleted Cells Is Accompanied by Coilin Downregulation and the Accumulation of Cajal Bodies in Nucleoli

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