The regulation of transcriptome responses in zebrafish embryo exposure to triadimefon

Abstract

The residue of triadimefon (TDF) (a pesticide) has become the pollutant in water due to its intensive use in agriculture and medicine, and its stability in water leaching from soil and vegetation. In this study, RNA-seq, a high-throughput method was performed, to analyze the global expression of differential expressed genes (DEGs) in zebrafish embryos treated with TDF (10 μg/mL) from fertilization to 72 h post-fertilization (hpf) as compared with that in the control group (without TDF treatment). Two cDNA libraries were generated from treated and non-treated embryos, respectively. With the 79.4% and 78.8% of reads mapped to the reference, it was observed that many differential genes were expressed between the two libraries. The most 20 differentially expressed up-regulated or down-regulated genes were involving in the signaling transduction, the activation of many genes related to cytochrome P450 enzymes, and molecular metabolism. Validation of seven genes expression confirmed RNA-seq results. The transcriptome sequences were further subjected to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis and showed diverse biological functions and metabolic pathways. The data from this study contributed to a better understanding of the potential consequences of fish exposed to TDF, and to evaluate the potential threat of TDF to fish population in the aquatic environment. © 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 217-226, 2017.

A colloidal gold immunochromatography test strip based on a monoclonal antibody for the rapid detection of triadimefon and triadimenol in foods

“…TDF is absorbed by all parts of the plant, where it inhibits ergosterol biosynthesis (Zhang et al, 2020), mycelium growth, and spore formation. According to the literature, TDF and its metabolite TDN can lead to reproductive toxicity, genotoxicity, neurotoxicity, teratogenicity, and carcinogenicity in humans (Cao, Wang, Jiao, & Zhu, 2016;Hsu, Chiou, Hsu, Wang, & Chen, 2017;Lenkowski, Sanchez-Bravo, & McLaughlin, 2010;Liang et al, 2013;Yinghong, Jing, Mingrong, Qiang, & Hu, 2018).…”

Triazole Fungicides Inhibit Zebrafish Hatching by Blocking the Secretory Function of Hatching Gland Cells

“…Unfortunately, in mammals, they also impair steroidogenesis therefore affecting reproduction and development [35] and there is substantial literature on the impacts of FON exposure to the endocrine system of mammals [36,37,38,39] and fish [32,40,41,42]. Additional work has described the teratogenic effects induced by FON and NOL exposure in ascidians [43], amphibians [44], and murine mammals [45].…”

“…The teratogenic effects of triazoles have been linked to alterations of retinoid acid (RA) metabolism and signaling, which may interfere with normal migration of the neural crest cells [46]. In zebrafish, the effects of FON, include reduction of growth, breeding success, and egg viability; teratogenesis; edema; and a decrease in hatching rates [32,40,41,47]. …”

Proteomic analysis of ametryn toxicity in zebrafish embryos

“…The zebrafish (Danio rerio) AB strain was provided by the Zebrafish Core at Academia Sinica, Institute of Cellular and Organism Biology (ICOB) in Taipei City, Taiwan, and the Taiwan Zebrafish Core Facility at the National Health Research Institute (NHRI) in Zhunan, Miaoli County, Taiwan. Zebrafish maintenance and embryo exposure was conducted according to the protocols of Hsu et al All animal handling protocols were approved by the Chung Shan Medical University Animal Care Committee, Taichung City, Taiwan. Fifty normal embryos were randomly distributed into glass beakers containing 500 mL of AMT (Sigma‐Aldrich, St. Louis, Missouri) at different doses (0, 10, or 20 µg/mL in 0.1% DMSO) until 24 and 48 hpf, respectively.…”