Auxin and Cellular Elongation

Velasquez, Silvia M.; Barbez, Elke; Kleine‐Vehn, Jürgen; Estevez, José M.

doi:10.1104/pp.15.01863

Cited by 94 publications

(73 citation statements)

References 124 publications

(136 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The study of these components in different organ/cell scenarios has been useful to reveal their involvement in cellular processes such as cell division, cell enlargement (Velasquez et al, 2016), cell differentiation (Yang and Wang, 2016), and subcellular processes such as remodeling of the cell wall (Pacheco-Villalobos et al, 2016) and lignin biosynthesis (Zhang et al, 2014). Most of them occur at some stage of strawberry fruit development.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Analysis in Strawberry Fruits Reveals Active Auxin Biosynthesis and Signaling in the Ripe Receptacle

et al. 2017

View full text Add to dashboard Cite

The role of auxin in ripening strawberry (Fragaria ×ananassa) fruits has been restricted to the early stages of development where the growth of the receptacle is dependent on the delivery of auxin from the achenes. At later stages, during enlargement of the receptacle, other hormones have been demonstrated to participate to different degrees, from the general involvement of gibberellins and abscisic acid to the more specific of ethylene. Here we report the involvement of auxin at the late stages of receptacle ripening. The auxin content of the receptacle remains constant during ripening. Analysis of the transcriptome of ripening strawberry fruit revealed the changing expression pattern of the genes of auxin synthesis, perception, signaling and transport along with achene and receptacle development from the green to red stage. Specific members of the corresponding gene families show active transcription in the ripe receptacle. For the synthesis of auxin, two genes encoding tryptophan aminotransferases, FaTAA1 and FaTAR2, were expressed in the red receptacle, with FaTAR2 expression peaking at this stage. Transient silencing of this gene in ripening receptacle was accompanied by a diminished responsiveness to auxin. The auxin activity in the ripening receptacle is supported by the DR5-directed expression of a GUS reporter gene in the ripening receptacle of DR5-GUS transgenic strawberry plants. Clustering by co-expression of members of the FaAux/IAA and FaARF families identified five members whose transcriptional activity was increased with the onset of receptacle ripening. Among these, FaAux/IAA11 and FaARF6a appeared, by their expression level and fold-change, as the most likely candidates for their involvement in the auxin activity in the ripening receptacle. The association of the corresponding ARF6 gene in Arabidopsis to cell elongation constitutes a suggestive hypothesis for FaARF6a involvement in the same cellular process in the growing and ripening receptacle.

show abstract

Section: Discussionmentioning

confidence: 99%

Transcriptomic Analysis in Strawberry Fruits Reveals Active Auxin Biosynthesis and Signaling in the Ripe Receptacle

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Based on the observation that overexpression of Arabidopsis AtAPY1 or AtAPY2 promotes auxin transport, we hypothesized that ectopic expression of their closest homologue in peas, psNTP9 , could result in a more extensive root system architecture (RSA). Multiple studies have shown that increased auxin transport would be likely to increase the growth and development of lateral roots and root hairs (Contreras‐Cornejo, Macías‐Rodríguez, Cortés‐Penagos, & López‐Bucio, ; Reed, Brady, & Muday, ; Velasquez, Barbez, Kleine‐Vehn, & Estevez, ). Moreover, suppressing the expression of AtAPY1 and AtAPY2 in Arabidopsis inhibits lateral root production (Wu et al, ).…”

Section: Introductionmentioning

confidence: 99%

Ectopic expression of a pea apyrase enhances root system architecture and drought survival in Arabidopsis and soybean

Veerappa

Slocum

Siegenthaler

et al. 2018

Plant Cell & Environment

View full text Add to dashboard Cite

Ectoapyrases (ecto-NTPDases) function to decrease levels of extracellular ATP and ADP in animals and plants. Prior studies showed that ectopic expression of a pea ectoapyrase, psNTP9, enhanced growth in Arabidopsis seedlings and that the overexpression of the two Arabidopsis apyrases most closely related to psNTP9 enhanced auxin transport and growth in Arabidopsis. These results predicted that ectopic expression of psNTP9 could promote a more extensive root system architecture (RSA) in Arabidopsis. We confirmed that transgenic Arabidopsis seedlings had longer primary roots, more lateral roots, and more and longer root hairs than wild-type plants. Because RSA influences water uptake, we tested whether the transgenic plants could tolerate osmotic stress and water deprivation better than wild-type plants, and we confirmed these properties. Transcriptomic analyses revealed gene expression changes in the transgenic plants that helped account for their enhanced RSA and improved drought tolerance. The effects of psNTP9 were not restricted to Arabidopsis, because its expression in soybeans improved the RSA, growth, and seed yield of this crop and supported higher survival in response to drought. Our results indicate that in both Arabidopsis and soybeans, the constitutive expression of psNTP9 results in a more extensive RSA and improved survival in drought stress conditions.

show abstract

“…Defects in pectin metabolism appear particularly prone to trigger CWI responses that activate the brassinosteroid pathway, leading to diverse growth phenotypes (Wolf et al, 2012(Wolf et al, , 2014. On the other hand, FERONIA and its extracellular peptide ligand ("rapid alkalinization factor") are also required for normal root growth and auxin responses (Haruta et al, 2014;Shih et al, 2014;Velasquez et al, 2016;Barbez et al, 2017). Cell expansion thus appears to be intimately linked to these wall sensor pathways in ways we are only beginning to fathom.…”

mentioning

confidence: 99%

“…Diffuse growth is the dominant pattern for most cells in the plant body and is traditionally contrasted with tip growth, for instance, in pollen tubes and root hairs, where surface expansion is localized to limited regions of the hemispherical tip (Campàs et al, 2012;Velasquez et al, 2016). Despite differences in spatial patterning, tip growth may involve some of the same cell wall processes as occur in diffuse growth, but in a more intense, spatially localized manner.…”

mentioning

confidence: 99%