Anti-Mycobacterium tuberculosis Activity of Calophyllum brasiliense Extracts Obtained by Supercritical Fluid Extraction and Conventional Techniques

Pires, Cláudia Terêncio Agostinho; Scodro, Regiane Bertin de Lima; Brenzan, Mislaine Adriana; Cortez, Diógenes Aparı́cio Garcia; Siqueira, Vera Lúcia Dias; Cardozo‐Filho, Lúcio; Gonçalves, Renata Menoci; Caleffi-Ferracioli, Katiany Rizzieri; Cardoso, Rosilene Fressatti

doi:10.2174/1389201017666160114094723

Cited by 3 publications

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“…The genus Calophyllum, belonging to the family Guttiferae which comprises about 200 species that are widely distributed in Asia, America and Africa [8], [9]. This genus has been shown to possess various pharmacological activities including antiarthritic [10], antileishmanial [11], vasorelaxation [12], antiinflammatory, antioxidant [13], antibacteria [14], cytotoxicity [15], antidiabetic [16], antimicrobial [17], antiseptics, astringents, diuretics, purgatives, anti-HIV and antifungal [18]. The genus Calophyllum has an abundant source of secondary metabolites, especially xanthones, coumarins, flavonoids, acylphloroglucinols, terpenoids and chromanones [ 12] , [ 19] , [ 20] .…”

Section: Paclitaxel Camptothecin Quininementioning

confidence: 99%

CHEMICAL CONSTITUENTS FROM THE ROOTS OF Calophyllum calaba L.

Laopian

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Phytochemical investigation of the CH2Cl2 extract from the roots of C. calaba let to the isolation of three new xanthone derivatives, namely calaxanthones A-C (1-3), along with twenty nine known xanthones (4-32). The structures of all isolated compounds were fully characterized using spectroscopic data (1D and 2D NMR) as well as comparison with the previous literature data. Moreover, all isolated compounds were assessed for their in vitro cytotoxicity against the KB, HeLa S-3, HT29, MCF-7 and HepG2 human cancer cell lines. The tested compounds mostly showed moderate to inactive against these five cell lines, except compounds 3 showed potent cytotoxicity against KB, HeLa S-3, HT29, MCF-7 and HepG2 cells with IC50 values of 1.72, 0.82, 1.17, 5.04 and 1.65 ?M, respectively. Furthermore, compound 6 showed good cytotoxicity against KB and HeLa S-3 cell with IC50 value of 7.06 and 5.27 ?M, respectively. Moreover, compound 13 showed good cytotoxicity against only KB cell with IC50 value of 4.62 ?M.

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Section: Paclitaxel Camptothecin Quininementioning

confidence: 99%

CHEMICAL CONSTITUENTS FROM THE ROOTS OF Calophyllum calaba L.

Laopian

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Network Pharmacology Uncovers Anticancer Activity of Mammea-Type Coumarins from Calophyllum brasiliense

et al. 2018

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Mammea-type coumarins are a particular type of secondary metabolites biosynthesized by the tropical rainforest tree , which is distributed from South America to Mexico. Particularly, mammea A/BA and A/BB (alone or as a mixture) possess biological properties such as cytotoxic and antitumoral activities, however, most of its molecular targets remain unknown. In this context, novel bioinformatic approaches, such as network pharmacology analysis, have been successfully used in herbal medicine to accelerate research in this field, and the support of experimental validations has been shown to be quite robust. In the present study, we performed a network pharmacology analysis to assess the possible molecular biological networks that interact with mammea A/BA and A/BB. Moreover, we validated the most relevant networks experimentally on K562 cancer cells. The results of the network pharmacology analysis indicate that mammea A/BA and A/BB interacts with cell death, PI3K/AKT, MAPK, Ras, and cancer pathways. The model shows that mammea A/BA and A/BB induce apoptosis through the overexpression of the proapoptotic proteins Bax and Bak, disrupt the autophagic flux as seen by the cytosolic accumulation of LC3-II and p62, disrupting the mitochondria ultrastructure and concomitantly increase the intracellular calcium concentration. Additionally, docking analysis predicted a possible interaction with a rapamycin-binding domain of mTOR. In conclusion, we validated network pharmacology analysis and report, for the first time, that mammea A/BA and A/BB coumarins induce apoptosis through the inhibition of the autophagic flux, possibly interacting with mTOR.

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Calophyllum brasilienseModulates the Immune Response and PromotesLeishmania amazonensisIntracellular Death

Domeneghetti

Demarchi

Caitano

et al. 2018

Mediators of Inflammation

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Calophyllum brasiliense is a plant from the Brazilian rain forests and has been used in folk medicine for the treatment of various diseases, including leishmaniasis. This infectious disease depends on the Leishmania sp. and the host immune response. C. brasiliense antileishmanial activity is well known, but the effects on immune response remain to be investigated. This study showed the leishmanicidal and immunomodulatory effects of a 30 μg/mL of hydroalcoholic extract of C. brasiliense in murine macrophages before and after Leishmania (Leishmania) amazonensis infection. The semiquantitative cytokine RNA expression was determined by RT-PCR and the anti-Leishmania activity was measured by infection index (IF). Hydroalcoholic extract of C. brasiliense reduced more than 95% of IF when used before and after Leishmania infection, with 3 and 24 h of treatment (p < 0.05). C. brasiliense inhibited or reduced significantly (p < 0.05) the TNF-α, IL-1β, IL-18, and IL-10 mRNA expression. The antileishmanial and anti-inflammatory effects showed the potential of C. brasiliense as an alternative therapy for leishmaniasis and it must be investigated.

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Anti-Mycobacterium tuberculosis Activity of Calophyllum brasiliense Extracts Obtained by Supercritical Fluid Extraction and Conventional Techniques

Cited by 3 publications

References 0 publications

CHEMICAL CONSTITUENTS FROM THE ROOTS OF Calophyllum calaba L.

CHEMICAL CONSTITUENTS FROM THE ROOTS OF Calophyllum calaba L.

Network Pharmacology Uncovers Anticancer Activity of Mammea-Type Coumarins from Calophyllum brasiliense

Calophyllum brasilienseModulates the Immune Response and PromotesLeishmania amazonensisIntracellular Death

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