Fabrication of highly catalytic silver nanoclusters/graphene oxide nanocomposite as nanotag for sensitive electrochemical immunoassay

Wang, Jiamian; Wang, Xiuyun; Wu, Shuo; Song, Jié; Zhao, Yanqiu; Ge, Yanqiu; Meng, Changgong

doi:10.1016/j.aca.2015.12.018

Cited by 22 publications

(14 citation statements)

References 41 publications

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“…5b); the bare electrode and His@AuNCs/RGOmodified electrode had a very low resistance for the redox probe. These results indicated that the redox reaction occurred more easily on the modified electrode surface, which implied that the stacked RGO sheets might act as a barrier to interfacial electron transfer compared to AuNCs [50,51]. Figure S5 (see ESM) shows typical voltammetric profiles for nitrite oxidation using GCE (curve d), RGO-GCE (curve c), His@AuNCs-GCE (curve b), and His@AuNCs/RGO- − in the presence of TMB.…”

Section: Fabrication and Electrochemical Performance Of Modified Elecmentioning

confidence: 98%

Enhanced His@AuNCs oxidase-like activity by reduced graphene oxide and its application for colorimetric and electrochemical detection of nitrite

Wen-e

et al. 2019

Anal Bioanal Chem

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Enzyme-mimicking (nanozyme)-based biosensors are attractive owing to their unique catalytic efficiency, multifunctionality, and tunable activity, but examples of oxidase-like nanozymes are quite rare. Herein, we demonstrated that histidine-capped gold nanoclusters (His@AuNCs) possessed intrinsic oxidase-like activity, which could directly oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) to blue colored ox-TMB without H 2 O 2. The assembly of reduced graphene oxide (RGO) with His@AuNCs could further improve its oxidase-like activity and the His@AuNCs/RGO nanocomposites had a lower Michaelis constant (K m) and higher catalytic constant (K cat) for TMB oxidation. Furthermore, compared to other nanomaterials, the as-prepared His@AuNCs/RGO also exhibited enhanced electrocatalytic activity toward TMB. Interestingly, nitrite inhibited the catalytic (chromogenic) and electrocatalytic processes of His@AuNCs/RGO in the oxidation of TMB. The oxidase-like and electrocatalytic activity of His@AuNCs/RGO was evaluated with nitrite and TMB as substrates, and the results indicated that TMB and nitrite might share the same catalytic active sites. On the basis of these findings, a colorimetric and electrochemical sensor was developed with the His@AuNCs/RGO composite as an oxidase mimic for determination of nitrite with linear ranges of 10-500 μM and 2.5-5700 μM, respectively. The developed method was successfully applied to the detection of nitrites in real samples. The present work suggests that the oxidase-like nanozyme is not only suitable for colorimetric assay but also for development of electrochemical sensors in bioanalysis.

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Section: Fabrication and Electrochemical Performance Of Modified Elecmentioning

confidence: 98%

Enhanced His@AuNCs oxidase-like activity by reduced graphene oxide and its application for colorimetric and electrochemical detection of nitrite

Wen-e

et al. 2019

Anal Bioanal Chem

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“…Moreover, the limit of detection (LOD) was estimated to be 5 pg/mL. The LOD is comparable to or even lower than that achieved by other immunosensors via the signal amplification of nanocatalyst labels ( Table S1 ) [ 41 , 42 , 43 , 44 , 45 , 46 ]. The high sensitivity can be attributed to the low background signal of the TCEP substrate, the signal amplification with CNTs as the carrier, and the high turnover frequency between FcM + and PQQH 2 as well as PQQ and TCEP.…”

Section: Resultsmentioning

confidence: 80%

Electrochemical Immunosensors with PQQ-Decorated Carbon Nanotubes as Signal Labels for Electrocatalytic Oxidation of Tris(2-carboxyethyl)phosphine

Deng

Xia

et al. 2021

Nanomaterials

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Nanocatalysts are a promising alternative to natural enzymes as the signal labels of electrochemical biosensors. However, the surface modification of nanocatalysts and sensor electrodes with recognition elements and blockers may form a barrier to direct electron transfer, thus limiting the application of nanocatalysts in electrochemical immunoassays. Electron mediators can accelerate the electron transfer between nanocatalysts and electrodes. Nevertheless, it is hard to simultaneously achieve fast electron exchange between nanocatalysts and redox mediators as well as substrates. This work presents a scheme for the design of electrochemical immunosensors with nanocatalysts as signal labels, in which pyrroloquinoline quinone (PQQ) is the redox-active center of the nanocatalyst. PQQ was decorated on the surface of carbon nanotubes to catalyze the electrochemical oxidation of tris(2-carboxyethyl)phosphine (TCEP) with ferrocenylmethanol (FcM) as the electron mediator. With prostate-specific antigen (PSA) as the model analyte, the detection limit of the sandwich-type immunosensor was found to be 5 pg/mL. The keys to success for this scheme are the slow chemical reaction between TCEP and ferricinum ions, and the high turnover frequency between ferricinum ions, PQQ. and TCEP. This work should be valuable for designing of novel nanolabels and nanocatalytic schemes for electrochemical biosensors.

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“…In a different study, Ag NCs labeled with a detection antibody were synthesized and loaded on GO for electrochemically sensing carcinoembryonic antigen (CEA), a tumor marker involved in many cancer types . The GO sheets, due to their large surface area, were utilized as a substrate for loading high amounts of Ag NCs , while also facilitating fast electron transfer through non‐covalent π‐π stacking interactions between sp 2 carbon atoms and bases of DNA antibody due to their excellent electron transport properties (Figure ).…”

Section: Applications Of Metal Nanoclusters/graphene Hybrid Materialsmentioning

confidence: 99%

“…Along the same lines, Ag NCs /GO composites were prepared and employed as a sensitive acetylcholinesterase biosensor . The novelty of this particular biosensor relied on the conductivity and electron‐transport properties of RGO as well as on the catalytic activity and biocompatibility of Ag NCs .…”

Section: Applications Of Metal Nanoclusters/graphene Hybrid Materialsmentioning

confidence: 99%

Hybrids of Metal Nanoclusters and Graphene‐Based Materials: Preparation, Properties and Applications

Koklioti

Tagmatarchis

2016

ChemNanoMat

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There is a significant drive within the scientific community to meet the demanding requirements of advanced energy‐conversion and detection schemes. The advantageous features of incorporating graphene with sub‐nanometer‐sized metal nanoclusters (MNCs) have already revealed encouraging results in key areas of selective sensing of hazardous molecules, detection of biologically related structures, bioimaging and hydrogen evolution. This timely Focus Review explores recent progress in the preparation and properties of MNCs/graphene hybrid materials, mainly focusing on the discussion of their applications. Moreover, a brief outlook on the future development of MNCs/graphene hybrid materials aiming to broaden their spectrum of applications is also provided.

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Fabrication of highly catalytic silver nanoclusters/graphene oxide nanocomposite as nanotag for sensitive electrochemical immunoassay

Cited by 22 publications

References 41 publications

Enhanced His@AuNCs oxidase-like activity by reduced graphene oxide and its application for colorimetric and electrochemical detection of nitrite

Enhanced His@AuNCs oxidase-like activity by reduced graphene oxide and its application for colorimetric and electrochemical detection of nitrite

Electrochemical Immunosensors with PQQ-Decorated Carbon Nanotubes as Signal Labels for Electrocatalytic Oxidation of Tris(2-carboxyethyl)phosphine

Hybrids of Metal Nanoclusters and Graphene‐Based Materials: Preparation, Properties and Applications

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