Dysregulation of sirtuins and key metabolic genes in skeletal muscle of pigs with spontaneous intrauterine growth restriction is associated with alterations of circulating IGF-1

Chriett, Sabrina; Huërou‐Luron, Isabelle Le; Vidal, Hubert; Pirola, Luciano

doi:10.1016/j.ygcen.2015.12.028

Cited by 9 publications

(9 citation statements)

References 45 publications

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“…5a). The expression of the miRNAs 24 18 , 19b 19 , 34a 20 , 122 21 , and 192 22 increased more than five-fold in exosomes from the PA-treated Huh7 cells, compared to those from vehicle-treated Huh7 cells. We next examined the expression levels of miRNA 122 and miRNA 192 using real-time PCR.…”

Section: Resultsmentioning

confidence: 88%

Exosomes derived from palmitic acid-treated hepatocytes induce fibrotic activation of hepatic stellate cells

Lee

Kim

et al. 2017

Sci Rep

178

173

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Non-alcoholic fatty liver disease (NAFLD) is a dominant cause of chronic liver disease, but the exact mechanism of progression from simple steatosis to nonalcoholic steatohepatitis (NASH) remains unknown. Here, we investigated the role of exosomes in NAFLD progression. Exosomes were isolated from a human hepatoma cell line treated with palmitic acid (PA) and their miRNA profiles examined by microarray. The human hepatic stellate cell (HSC) line (LX-2) was then treated with exosome isolated from hepatocytes. Compared with controls, PA-treated hepatocytes displayed significantly increased CD36 and exosome production. The microarray analysis showed there to be distinctive miRNA expression patterns between exosomes from vehicle- and PA-treated hepatocytes. When LX-2 cells were cultured with exosomes from PA-treated hepatocytes, the expression of genes related to the development of fibrosis were significantly amplified compared to those treated with exosomes from vehicle-treated hepatocytes. In conclusion, PA treatment enhanced the production of exosomes in these hepatocytes and changed their exosomal miRNA profile. Moreover, exosomes derived from PA-treated hepatocytes caused an increase in the expression levels of fibrotic genes in HSCs. Therefore, exosomes may have important roles in the crosstalk between hepatocytes and HSCs in the progression from simple steatosis to NASH.

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Section: Resultsmentioning

confidence: 88%

Exosomes derived from palmitic acid-treated hepatocytes induce fibrotic activation of hepatic stellate cells

Lee

Kim

et al. 2017

Sci Rep

178

173

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“…This conclusion is supported by recent studies that have found methylation at promoters is less variable over the lifespan than other regions, such as enhancers, suggesting that methylation at nonpromoter regions may be more environmentally sensitive. 57,58 In addition, though the positive association between IGF serum levels in both mothers and infants and birth weight has been well documented in both animal 59 and human studies, 23,25 the literature reporting a relationship between neonatal growth measures and methylation in IGF1, particularly across tissue sites has been less consistent and at times, contradictory. 26,31,32,56 Although the significance of our results with IGF1 methylation was attenuated following correction for multiple testing, Bonferroni correction is a very conservative method.…”

Section: Discussionmentioning

confidence: 99%

Associations between maternal prenatal stress, methylation changes in IGF1 and IGF2, and birth weight

Montoya-Williams

Quinlan

Clukay

et al. 2017

J Dev Orig Health Dis

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Maternal stress has been linked to low birth weight in newborns. One potential pathway involves epigenetic changes at candidate genes that may mediate the effects of prenatal maternal stress on birth weight. This relationship has been documented in stress-related genes, such as NR3C1. There is less literature exploring the effect of stress on growth-related genes. IGF1 and IGF2 have been implicated in fetal growth and development, though via different mechanisms as IGF2 is under imprinting control. In this study, we tested for associations between prenatal stress, methylation of IGF1 and IGF2, and birth weight. A total of 24 mother-newborn dyads in the Democratic Republic of Congo were enrolled. Ethnographic interviews were conducted with mothers at delivery to gather culturally relevant war-related and chronic stressors. DNA methylation data were generated from maternal venous, cord blood and placental tissue samples. Multivariate regressions were used to test for associations between stress measures, DNA methylation and birth weight in each of the three tissue types. We found an association between IGF2 methylation in maternal blood and birth weight. Previous literature on the relationship between IGF2 methylation and birth weight has focused on methylation at known differentially methylated regions in cord blood or placental samples. Our findings indicate there may be links between the maternal epigenome and low birth weight that rely on mechanisms outside known imprinting pathways. It thus may be important to consider the effect of maternal exposures and epigenetic profiles on birth weight even in the setting of maternally imprinted genes such as IGF2.

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“…Sirtuins are a family of cellular sensors exerting a deacetylase function which regulates cell bioenergetics by synchronizing nuclear and mitochondrial activities (32)(33)(34)(35). Despite evidence of deregulation of sirtuins in skeletal muscle of pigs with spontaneous IUGR (36), little is known about their implication in this obstetric complication. Interestingly, growing evidence supports sirtuins involvement in cardiac disease, independent of IUGR, either by modulation of bioenergetic shift and cardiomyocyte survival (37).…”

mentioning

confidence: 99%

Cardiac and placental mitochondrial characterization in a rabbit model of intrauterine growth restriction

Guitart‐Mampel

Gonzalez-Tendero

Niñerola

et al. 2018

Biochimica et Biophysica Acta (BBA) - General Subjects

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Dysregulation of sirtuins and key metabolic genes in skeletal muscle of pigs with spontaneous intrauterine growth restriction is associated with alterations of circulating IGF-1

Cited by 9 publications

References 45 publications

Exosomes derived from palmitic acid-treated hepatocytes induce fibrotic activation of hepatic stellate cells

Exosomes derived from palmitic acid-treated hepatocytes induce fibrotic activation of hepatic stellate cells

Associations between maternal prenatal stress, methylation changes in IGF1 and IGF2, and birth weight

Cardiac and placental mitochondrial characterization in a rabbit model of intrauterine growth restriction

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