Circulating ANGPTL8/Betatrophin Concentrations Are Increased After Surgically Induced Weight Loss, but Not After Diet-Induced Weight Loss

Pascual‐Corrales, Eider; Gómez-Ambrosi, Javier; Moncada, Rafael; Valentí, Víctor; Catalán, Victoria; Rodríguez, Amaia; Ramírez, Beatriz; Silva, Camilo; Gil, Marı́a J.; Salvador, Javier; Frühbeck, Gema

doi:10.1007/s11695-015-2026-7

Cited by 24 publications

(18 citation statements)

References 44 publications

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“…On the other hand, we have studied the correlation of ANGPTL8 with some metabolic parameters; in our case we have found a positive correlation in obese patients of ANGPTL8 and HDL-cholesterol. This data is in agreement with other publications in which the change in ANGPTL8/betatrophin levels was positively correlated with the change in HDL-C concentrations in T2DM [18] and obese patients [53]. In obese women, it proposes that ANGPTL8 has a potential role in dyslipidaemia, and is intimately related to HDL [34].…”

Section: Discussionsupporting

confidence: 93%

Evidences for Expression and Location of ANGPTL8 in Human Adipose Tissue

Catalano-Iniesta

Robledo

Iglesias-Osma

et al. 2020

JCM

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The metabolism of triglycerides (TGs) is regulated, among others, by the lipoprotein lipase (LPL) that hydrolyses the TGs on endothelial cells. In turn, LPL is inhibited by the ANGPTLs family of proteins, such as ANGPTL3, 4, and, 8; the latter is the least known. In this work, we have tried to establish the expression and localisation of the Angiopoietin-like 8 (ANGPTL8) protein in the visceral adipose tissue (VAT) of morbid-obese and non-obese patients. 109 subjects (66 women and 43 men) undergoing laparoscopic surgery participated in this study. A blood sample and a portion of the VAT were obtained, and the patients were classified according to their Body Mass Index (BMI) as non-obese (19.5–30 kg/m2) and morbid-obese (40–50 kg/m2). No significant changes in ANGPTL8 plasma levels were determined by EIA in obese patients. The immunocytochemistry and Western blotting showed the presence of increased ANGPTL8 in morbid-obese patients (p < 0.05). In-situ hybridisation and a real time polymerase chain reaction (RT-PCR) confirmed that the mRNA that encodes ANGPTL8 was present in adipocytes, without differences in their nutritional state (p = 0.89), and even in the endothelial cells. Our data suggests that ANGPT8 plasmatic levels do not change significantly in patients with morbid obesity, although there is a modest difference related to gender. Besides, we demonstrate that in visceral adipose tissue, ANGPTL8 is well defined in the cytoplasm of adipocytes coexisting with perilipin-1 and its mRNA, also is present in endothelial cells. These findings suggest the possibility that among other functions, ANGPTL8 could perform either a paracrine and/or an endocrine role in the adipose tissue.

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Section: Discussionsupporting

confidence: 93%

Evidences for Expression and Location of ANGPTL8 in Human Adipose Tissue

Catalano-Iniesta

Robledo

Iglesias-Osma

et al. 2020

JCM

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“…Some studies have explored the effect of weight change on betatrophin, presenting with inconsistent results. A study reported that serum betatrophin levels decrease after diet-related weight loss [16] while another noted that only surgeryinduced weight loss increases blood betatrophin levels [17].…”

Section: Introductionmentioning

confidence: 99%

Circulating betatrophin/ANGPTL8 levels correlate with body fat distribution in individuals with normal glucose tolerance but not those with glucose disorders

et al. 2020

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Background: The relationship between betatrophin/ANGPTL8 and obesity has been investigated using body mass index (BMI); however, since BMI reflects overall adiposity rather than body fat distribution, it remains unclear whether fat deposition in different areas of the body affects betatrophin expression. Here, we investigated the correlation between circulating betatrophin levels and body fat distribution in patients with different glucose tolerance. Methods: We performed a cross-sectional study in 128 participants with impaired glucose tolerance (IGT; n = 64) or normal glucose tolerance (NGT; n = 64). Circulating betatrophin levels were detected by enzyme-linked immunosorbent assay (ELISA). Body fat distribution (subcutaneous, visceral, and limb fat) was measured by magnetic resonance imaging (MRI) and a body fat meter.Results: After controlling for age, sex, and BMI, betatrophin was correlated positively with visceral adipose tissue-tosubcutaneous adipose tissue ratio (VAT/SAT ratio; r = 0.339, p = 0.009) and negatively with body fat ratio (BFR; r = − 0.275, p = 0.035), left lower limb fat ratio (LLR; r = − 0.330, p = 0.011), and right lower limb fat ratio (RLR; r = − 0.288, p = 0.027) in the NGT group, with these correlations remaining after controlling for triglycerides. VAT/SAT ratio (standardized β = 0.419, p = 0.001) was independently associated with serum betatrophin levels; however, betatrophin was not associated with body fat distribution variables in the IGT group.Conclusions: Circulating betatrophin levels correlated positively with VAT/SAT ratio and negatively with lower limb fat, but not with subcutaneous or upper limb fat, in individuals with normal glucose tolerance. Thus, betatrophin may be a potential biomarker for body fat distribution in individuals without glucose disorders.

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“…Nonetheless, given the connection between ANGPTL8 and obesity, it is reasonable that ANGPTL8 would be regulated by weight loss [17]. Recent weight loss studies have evaluated the effects of bariatric surgery [16,[18][19][20], diet [21,22], exercise [23], and diet plus exercise [13] on ANGPTL8 concentrations in diabetic and nondiabetic obese subjects. Unfortunately, contradictory results (increase [16,19], decrease [18,20,22,23] and not change [13,21]) have also been reported.…”

mentioning

confidence: 99%

Effects of a diet with or without physical activity on angiopoietin-like protein 8 concentrations in overweight/obese patients with newly diagnosed type 2 diabetes: a randomized controlled trial

Yuan

Wang

et al. 2019

Endocr J

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Angiopoietin-like protein 8 (ANGPTL8) is a newly discovered adipokine plays an important role in energy homoeostasis, obesity and type 2 diabetes (T2D). Although lifestyle modification in obesity and T2D is known to offer metabolic benefits, there is paucity of comprehensive data on change in ANGPTL8. We investigated the effect of lifestyle intervention on ANGPTL8 concentrations. 384 obese/overweight adults with newly diagnosed T2D were randomly assigned (1:1:1) to diet (n = 128), diet + activity (n = 128) or usual care (control, n = 128) groups. All patients received usual care. Besides, the diet group received a calorie-restricted diet aiming for a weight loss of 5-10%. The diet + activity group additionally received a pedometer-based walking program. Primary outcome was change in ANGPTL8 concentration at 6 months. Data were analyzed according to intention-to-treat. From baseline to 6 months, the median ANGPTL8 level changed from 804.38 pg/mL to 792.86 pg/mL in control group. Compared with control, ANGPTL8 decreased with diet (baselineadjusted between-group difference was-121.00 pg/mL, 95% CI-177.47 to-64.53; p < 0.0001) and diet + activity (-126.16 pg/mL,-181.21 to-71.11; p < 0.0001). There was no greater effect of diet + activity compared with diet (-5.16 pg/mL,-53.63 to 43.31; p = 0.8348). Both effects disappeared after adjusting for change in body fat, but did not differ significantly when adjusting for physical activity. A 6-month intervention inducing weight loss by a calorie-restricted diet or diet + activity, resulted in significant decrease on ANGPTL8 concentration. These effects were established by change in total body fat, and not by change in physical activity.

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Circulating ANGPTL8/Betatrophin Concentrations Are Increased After Surgically Induced Weight Loss, but Not After Diet-Induced Weight Loss

Cited by 24 publications

References 44 publications

Evidences for Expression and Location of ANGPTL8 in Human Adipose Tissue

Evidences for Expression and Location of ANGPTL8 in Human Adipose Tissue

Circulating betatrophin/ANGPTL8 levels correlate with body fat distribution in individuals with normal glucose tolerance but not those with glucose disorders

Effects of a diet with or without physical activity on angiopoietin-like protein 8 concentrations in overweight/obese patients with newly diagnosed type 2 diabetes: a randomized controlled trial

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