Correction: Escape from Lethal Bacterial Competition through Coupled Activation of Antibiotic Resistance and a Mobilized Subpopulation

Stubbendieck, Reed M.; Straight, Paul D.

doi:10.1371/journal.pgen.1005807

Cited by 25 publications

(2 citation statements)

References 2 publications

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“…occurs on top of 1.5% agar, which is a concentration 2-fold higher than the maximal concentration permitting sliding in B. subtilis monocultures (37), consistent with similar observations that were recently made for additional interspecies interactions between B. subtilis and Streptomyces spp. (38,39). This movement does not require flagellar motility or chemotaxis.…”

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confidence: 94%

The Plant Host Induces Antibiotic Production To Select the Most-Beneficial Colonizers

Ogran

Yardeni

Keren-Paz

et al. 2019

Appl Environ Microbiol

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Microbial ecosystems tightly associated with a eukaryotic host are widespread in nature. The genetic and metabolic networks of the eukaryotic hosts and the associated microbes have coevolved to form a symbiotic relationship. Both the Gram-positive Bacillus subtilis and the Gram-negative Serratia plymuthica can form biofilms on plant roots and thus can serve as a model system for the study of interspecies interactions in a host-associated ecosystem. We found that B. subtilis biofilms expand collectively and asymmetrically toward S. plymuthica, while expressing a nonribosomal antibiotic bacillaene and an extracellular protease. As a result, B. subtilis biofilms outcompeted S. plymuthica for successful colonization of the host. Strikingly, the plant host was able to enhance the efficiency of this killing by inducing bacillaene synthesis. In turn, B. subtilis biofilms increased the resistance of the plant host to pathogens. These results provide an example of how plant-bacterium symbiosis promotes the immune response of the plant host and the fitness of the associated bacteria. IMPORTANCE Our study sheds mechanistic light on how multicellular biofilm units compete to successfully colonize a eukaryote host, using B. subtilis microbial communities as our lens. The microbiota and its interactions with its host play various roles in the development and prevention of diseases. Using competing beneficial biofilms that are essential microbiota members on the plant host, we found that B. subtilis biofilms activate collective migration to capture their prey, followed by nonribosomal antibiotic synthesis. Plant hosts increase the efficiency of antibiotic production by B. subtilis biofilms, as they activate the synthesis of polyketides; therefore, our study provides evidence of a mechanism by which the host can indirectly select for beneficial microbiota members.on July 10, 2020 by guest http://aem.asm.org/ Downloaded from RESULTSB. subtilis biofilm actively expands toward a competing S. plymuthica colony. When grown on a solid biofilm-inducing medium, B. sutbilis biofilms form symmetrical, circular colonies. To determine the effect of a competing S. plymuthica colony on the development of a B. subtilis biofilm, the two species were inoculated next to each other on a solid biofilm medium. After 2 days, the B. subtilis colony reached S. plymuthica, forming a thick wrinkle around its edge and penetrating toward its center. By the third day, B. subtilis biofilm completely engulfed the S. plymuthica colony, covering it with a thin, unstructured film and enclosing it within the circular wrinkle (Fig. 1A). The location and shape of the B. subtilis center of the biofilm did not change during the interaction with S. plymuthica. However, the biofilm colony advanced asymmetrically toward the S. plymuthica colony, breaking from its usual circular shape ( Fig. 1A).We next examined the mechanisms that could mediate this asymmetric expansion toward a competitor. Many bacterial cells are capable of directional swimming using their flagella (2...

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confidence: 94%

The Plant Host Induces Antibiotic Production To Select the Most-Beneficial Colonizers

Ogran

Yardeni

Keren-Paz

et al. 2019

Appl Environ Microbiol

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“…subtilis and Streptomyces sp. Mg1 (S. Mg1) (Stubbendieck & Straight, 2016) and found that strains of B. subtilis without lnrL are lysed while those who had lnrL could counter the lytic stress. Defense genes like this one may be important in promoting the competitive fitness of the bacteria.…”

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confidence: 99%

Embracing Change: A Look into the Ecological Diversification of Bacillus atrophaeus in Death Valley National Park using Whole-Genome Analysis

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Label-Free Proteomics of a Defined, Binary Co-culture Reveals Diversity of Competitive Responses Between Members of a Model Soil Microbial System

et al. 2017

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Interactions among members of microbial consortia drive the complex dynamics in soil, gut, and biotechnology microbiomes. Proteomic analysis of defined co-cultures of well-characterized species provides valuable information about microbial interactions. We used a label-free approach to quantify the responses to co-culture of two model bacterial species relevant to soil and rhizosphere ecology, Bacillus atrophaeus and Pseudomonas putida. Experiments determined the ratio of species in co-culture that would result in the greatest number of high-confidence protein identifications for both species. The 281 and 256 proteins with significant shifts in abundance for B. atrophaeus and P. putida, respectively, indicated responses to co-culture in overall metabolism, cell motility, and response to antagonistic compounds. Proteins associated with a virulent phenotype during surface-associated growth were significantly more abundant for P. putida in co-culture. Co-culture on agar plates triggered a filamentous phenotype in P. putida and avoidance of P. putida by B. atrophaeus colonies, corroborating antagonistic interactions between these species. Additional experiments showing increased relative abundance of P. putida under conditions of iron or zinc limitation and increased relative abundance of B. atrophaeus under magnesium limitation were consistent with patterns of changes in abundance of metal-binding proteins during co-culture. These results provide details on the nature of interactions between two species with antagonistic capabilities. Significant challenges remaining for the development of proteomics as a tool in microbial ecology include accurate quantification of low-abundance peptides, especially from rare species present at low relative abundance in a consortium.

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Correction: Escape from Lethal Bacterial Competition through Coupled Activation of Antibiotic Resistance and a Mobilized Subpopulation

Abstract: There is an error in the bond configuration for the linearmycin B structure depicted in Fig 1D. Please view the correct figure here.

Cited by 25 publications

References 2 publications

The Plant Host Induces Antibiotic Production To Select the Most-Beneficial Colonizers

The Plant Host Induces Antibiotic Production To Select the Most-Beneficial Colonizers

Embracing Change: A Look into the Ecological Diversification of Bacillus atrophaeus in Death Valley National Park using Whole-Genome Analysis

Label-Free Proteomics of a Defined, Binary Co-culture Reveals Diversity of Competitive Responses Between Members of a Model Soil Microbial System

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