Epidemiology of human coronavirus NL63 infection among hospitalized patients with pneumonia in Taiwan

Huang, Su; Su, Mei Chi; Tien, Ni; Huang, Chien Jhen; Lan, Yu Ching; Lin, Chen Sheng; Chen, Chao Hsien; Lin, Cheng Wen

doi:10.1016/j.jmii.2015.10.008

Cited by 39 publications

(37 citation statements)

References 18 publications

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“…Human coronavirus (HCoV) NL63, the member of the genus alphacoronavirus in the Coronaviridae family, is one of common human coronaviruses (Li and Lin, 2013;Huang et al, 2017). HCoV-NL63 genome is a positive-strand RNA with near 27.5 kb nucleotides containing 5′ untranslated regions (UTR), ORF1a/b, spike(S), ORF3, envelope(E), membrane(M), nucleoprotein (N), and 3′UTR (Geng et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Antiviral activity of Sambucus FormosanaNakai ethanol extract and related phenolic acid constituents against human coronavirus NL63

et al. 2019

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Human coronavirus NL63 (HCoV-NL63), one of the main circulating HCoVs worldwide, causes respiratory tract illnesses like runny nose, cough, bronchiolitis and pneumonia. Recently, a severe respiratory illness outbreak of HCoV-NL63 has been reported in a long-term care facility. Sambucus FormosanaNakai, a species of elderberry, is a traditional medicinal herb with anti-inflammatory and antiviral potential. The study investigated the antiviral activity of Sambucus FormosanaNakai stem ethanol extract and some phenolic acid constituents against HCoV-NL63. The extract was less cytotoxic and concentration-dependently increased anti-HCoV-NL63 activities, including cytopathicity, sub-G1 fraction, virus yield (IC50 = 1.17 μg/ml), plaque formation (IC50 = 4.67 μg/ml) and virus attachment (IC50 = 15.75 μg/ml). Among the phenolic acid constituents in Sambucus FormosanaNakai extract, caffeic acid, chlorogenic acid and gallic acid sustained the anti-HCoV-NL63 activity that was ranked in the following order of virus yield reduction: caffeic acid (IC 50 = 3.54 μM) > chlorogenic acid (IC 50 = 43.45 μM) > coumaric acid (IC 50 = 71.48 μM). Caffeic acid significantly inhibited the replication of HCoV-NL63 in a cell-type independent manner, and specifically blocked virus attachment (IC 50 = 8.1 μM). Therefore, the results revealed that Sambucus Formosana Nakai stem ethanol extract displayed the strong anti-HCoV-NL63 potential; caffeic acid could be the vital component with anti-HCoV-NL63 activity. The finding could be helpful for developing antivirals against HCoV-NL63.

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Section: Introductionmentioning

confidence: 99%

Antiviral activity of Sambucus FormosanaNakai ethanol extract and related phenolic acid constituents against human coronavirus NL63

et al. 2019

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“…The etiology of CAP is diverse and complex; however, continuous identification of the major pathogens related to CAP is necessary for prevention and improved treatment . In this context, CAP is caused by a variety of pathogenic infectious agents including viruses and bacteria, of which viruses are the most predominant in children .…”

Section: Introductionmentioning

confidence: 99%

High incidence of rhinovirus infection in children with community‐acquired pneumonia from a city in the Brazilian pre‐Amazon region

Ferreira

Costa

Cariolano

et al. 2019

Journal of Medical Virology

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Community‐acquired pneumonia (CAP) is the leading cause of child death worldwide. Viruses are the most common pathogens associated with CAP in children, but their incidence varies greatly. This study investigated the presence of respiratory syncytial virus (RSV), adenovirus, human rhinovirus (HRV), human metapneumovirus (HMPV), human coronavirus (HCoV‐OC43 and HCoV‐NL63), and influenza A virus (FluA) in children with CAP and the contributing risk factors. Here, children with acute respiratory infections were screened by pediatrics; and a total of 150 radiographically‐confirmed CAP patients (aged 3 months to 10 years) from two clinical centers in Sao Luis, Brazil were recruited. Patient's clinical and epidemiological data were recorded. Nasopharyngeal swab and tracheal aspirate samples were collected to extract viral nucleic acid. RSV, adenovirus, rhinovirus, FluA, HMPV, HCoV‐OC43, and HCoV‐NL63 were detected by real‐time polymerase chain reaction. The severe CAP was associated with ages between 3 and 12 months. Viruses were detected in 43% of CAP patients. Rhinovirus infections were the most frequently identified (68%). RSV, adenovirus, FluA, and coinfections were identified in 14%, 14%, 5%, and 15% of children with viral infection, respectively. Rhinovirus was associated with nonsevere CAP (P = .014); RSV, FluA, and coinfections were associated with severe CAP (P < .05). New strategies for prevention and treatment of viral respiratory infections, mainly rhinovirus and RSV infections, are necessary.

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“…Severe acute respiratory syndrome (SARS)-associated coronavirus (CoV), a member of betacoronoviruses in the Coronaviridae family, is identified as the causative agent for the outbreak of SARS in Asia and other countries in [2002][2003]. Like other human coronaviruses (HCoVs) HCoV-229E, HCoV-OC43, HCoV-NL63, HCoV-HKU1, and MERS-CoV (Li and Lin, 2013;Huang et al, 2015), SARS-CoV genome is an approximately 30 kb positive-strand RNA consisting of a 5′ cap, a 3′ poly (A) tract, and 14 open reading frames (ORFs). The largest ORFs ORF1a and ORF1ab encode for the polyprotein replicases 1a and 1ab mainly involving in the SARS-CoV replication, as cleaved in cis and in trans by ORF1a-encoded papain-like protease (PLpro) and 3C-like protease (3CLpro).…”

Section: Introductionmentioning

confidence: 99%

SARS coronavirus papain-like protease up-regulates the collagen expression through non-Samd TGF-β1 signaling

Wang

et al. 2017

Virus Research

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SARS coronavirus (CoV) papain-like protease (PLpro) reportedly induced the production of TGF-β1 through p38 MAPK/STAT3-meidated Egr-1-dependent activation (Sci. Rep. 6, 25754). This study investigated the correlation of PLpro-induced TGF-β1 with the expression of Type I collagen in human lung epithelial cells and mouse pulmonary tissues. Specific inhibitors for TGF-βRI, p38 MAPK, MEK, and STAT3 proved that SARS-CoV PLpro induced TGF-β1-dependent up-regulation of Type I collagen in vitro and in vivo. Subcellular localization analysis of SMAD3 and SMAD7 indicated that non-SMAD pathways in TGF-β1 signaling involved in the production of Type I collagen in transfected cells with pSARS-PLpro. Comprehensive analysis of ubiquitin-conjugated proteins using immunoprecipitation and nanoLC-MS/MS indicated that SARS-CoV PLpro caused the change in the ubiquitination profile of Rho GTPase family proteins, in which linked with the increase of Rho-like GTPase family proteins. Moreover, selective inhibitors TGF-βRI and STAT6 (AS1517499) ascertained that STAT6 activation was required for PLpro-induced TGF-β1-dependent up-regulation of Type I collagen in human lung epithelial cells. The results showed that SARS-CoV PLpro stimulated TGF-β1-dependent expression of Type I collagen via activating STAT6 pathway.

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Epidemiology of human coronavirus NL63 infection among hospitalized patients with pneumonia in Taiwan

Abstract: Understanding epidemiology of HCoV-NL63 in Taiwan provides an insight for worldwide surveillance of HCoV-NL63 infection.

Cited by 39 publications

References 18 publications

Antiviral activity of Sambucus FormosanaNakai ethanol extract and related phenolic acid constituents against human coronavirus NL63

Antiviral activity of Sambucus FormosanaNakai ethanol extract and related phenolic acid constituents against human coronavirus NL63

High incidence of rhinovirus infection in children with community‐acquired pneumonia from a city in the Brazilian pre‐Amazon region

SARS coronavirus papain-like protease up-regulates the collagen expression through non-Samd TGF-β1 signaling

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