A new direct transfer protocol for cryopreserved IVF embryos

Sanches, B. V.; Lunardelli, Paula Alvares; Tannura, J. H.; Cardoso, Bruna Lopes; Pereira, M. H. C.; Gaitkoski, D.; Basso, Andrea; Arnold, Daniel; Seneda, Marcelo Marcondes

doi:10.1016/j.theriogenology.2015.11.029

Cited by 58 publications

(41 citation statements)

References 20 publications

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“…The absence of relevant variation in the pregnancy percentage of embryos produced in vitro for treated or non-FSH-treated cows can be characterized by the equivalent quality of the embryos transferred. The results observed in the present study are similar to those verified in the literature, with distinct breeds and protocols for obtaining oocytes (Guemra et al, 2014;Sanches et al, 2016).…”

Section: Discussionsupporting

confidence: 91%

Effect of single dose follicle stimulating hormone on follicular aspiration, in vitro fertilization and pregnancy rate

Moraes¹,

Adona²,

Guemra³

et al. 2019

Braz. J. Vet. Res. Anim. Sci.

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The present study evaluated Brangus cows treated with single doses of follicle stimulating hormone (FSH) subjected to follicular aspiration after 24 h to assess oocyte recovery, in vitro fertilization and pregnancy rate. Follicles exceeding 3 millimeters in diameter were aspirated, 200 mg of FSH was administered 2 days later, and a new ovum pickup was performed 24 h afterward. These methods were performed 3 times every 3 days. In control, follicular aspirations occurred at intervals of 1-week without FSH administration o. The aspirated oocytes were evaluated, submitted to in vitro fertilization and the embryos were transferred to the recipients. The average recovery of oocytes was higher (p<0.05) in control cows (12.4±1.8) than in treated cows (9.4±1.3). There was no difference (p>0.05) in the mean percentage of viable oocytes (52.0±3.9 and 62.7±4.7%) or the mean percentage of embryos (41.4±4.8 and 41.5±4.2%) among control and treated cows, respectively. The mean percentage of pregnancy did not differ (p>0.05) for control cows (43.8±2.7%), and treated cows (40.9±6.8%). In conclusion, FSH treatment did not improve oocyte recovery, in vitro fertilization, and pregnancy percentage. However, there is possibility of several consecutive ovum pickup every t3 days, concentrating the in vitro fertilization and the pregnancy percentage. Keywords: Bovine. Follicle. Ovum pickup. RESUMOO presente estudo avaliou vacas Brangus tratadas com doses únicas de hormônio folículo estimulante (FSH) submetidas a aspiração folicular após vinte e quatro horas, para avaliação da recuperação oocitária, fertilização in vitro e taxa de prenhez. Folículos superiores a três milímetros de diâmetro foram aspirados, 200 mg de FSH foram administrados dois dias depois e uma nova aspiração folicular foi realizada 24 horas após. Esses métodos foram efetivados três vezes a cada três dias. No controle, as aspirações foliculares ocorreram em intervalos de uma semana sem administração de FSH. Os oócitos aspirados foram avaliados, submetidos à fertilização in vitro e os embriões foram transferidos em receptoras. A recuperação média dos oócitos foi superior (p<0,05) nas vacas controle (12,4±1,8) do que nas vacas tratadas (9,4±1,3). Não houve diferença (p>0,05) na porcentagem média de oócitos viáveis (52,0±3,9 e 62,7±4,7%) ou na porcentagem média de embriões (41,4±4,8 e 41,5±4,2%) entre vacas controle e vacas tratadas, respectivamente. A porcentagem média de prenhez não diferiu (p>0,05) para as vacas controle (43,8±2,7%) e as tratadas (40,9±6,8%). Em conclusão, o tratamento com FSH não melhorou a recuperação de oócitos, a fertilização in vitro e o percentual de prenhez. No entanto, existe a possibilidade de várias aspirações foliculares consecutivas a cada três dias, concentrando a fertilização in vitro e o percentual de prenhez. Palavras-chave: Aspiração follicular. Bovinos. Folículos. 2/8 Braz J Vet Res Anim Sci. 2019;56(3):e156894 How to cite: Moraes MEB, Adona PR, Guemra S, De Bem THC, Miranda MS. Effect of single dose follicle stimulating hormone on fol...

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Section: Discussionsupporting

confidence: 91%

Effect of single dose follicle stimulating hormone on follicular aspiration, in vitro fertilization and pregnancy rate

Moraes¹,

Adona²,

Guemra³

et al. 2019

Braz. J. Vet. Res. Anim. Sci.

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“…Transferred embryos The results of this study revealed the possibility of using frozen embryos because the direct transfer optimized the logistics and can become a more practical approach for the transfer of cryopreserved in vitro embryos in the field (Sanches et al, 2016).…”

Section: Groupmentioning

confidence: 91%

“…Currently, slow freezing (classic) and vitrification (ultra-rapid) are the two main methods used commercially for IVEP embryo cryopreservation (Saragusty and Arav, 2011). Vitrification is the predominant technique used for IVEP (Dode et al, 2013) due to being a simple, fast and low-cost method (Sanches et al, 2016). In this method, a high-osmolarity solution is used so that the embryonic intracellular water exits rapidly, dehydrating the embryonic cells and making them permeable to the cryoprotectant.…”

Section: Cryopreservation Of Bovine Embryosmentioning

confidence: 99%

“…In this context, the total embryo production is sometimes higher than the number of embryos transferred, so investment in research was increased to develop an efficient protocol for the cryopreservation of the remaining embryos in a program (Sanches et al, 2016). Despite the advantages provided by IVEP, the greatest challenge of this biotechnology is the lower resistance to the cryopreservation process that these embryos present (Sudano et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…However, this technique requires a high concentration of cryoprotectants in addition to a trained person to perform a morphological evaluation of embryo quality before the loading process (Vajta et al, 1998). In contrast, Sanches et al (2016) demonstrated that a technique used since the 1990s to simplify the post-thawing rehydration step of in vivo embryosdirect transfer (DT) -can also be used for frozen in vitro embryos. The DT strategy has been demonstrated to be helpful for overcoming limitations to in vitro embryo cryopreservation, since it has been recently performed by commercial laboratories, providing good embryo viability after thawing.…”

Section: Introductionmentioning

confidence: 99%

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Cryopreservation of in vitro-produced embryos: challenges for commercial implementation

Sanches¹,

Zangirólamo²,

Silva³

et al. 2017

Anim Reprod

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In the last several years, the high demand for embryo production has resulted in the need to study new methods to make the cryopreservation of bovine embryos produced in vitro more efficient. Despite the advantages offered by in vitro embryo production (IVEP), the major challenge to its greater dissemination is to improve embryonic survival after cryopreservation. Embryos that are produced in vitro are less resistant to cryopreservation compared to those produced in vivo, which is due to the higher accumulation of lipids in their cells, among other factors. In this context, changes in the culture conditions such as the addition of lipolytic chemical substances and the adjustment of fetal calf serum in the medium have been proposed to decrease the lipid amount within the embryos. Several years ago, vitrification allowed good results for in vitro produced (IVP) embryos because of its simplicity, speed and low cost. More recently, another technique applied to simplify the embryo post-thawing rehydration step in vivo, direct transfer (DT), is a strategy that has proven to be of interest in helping to overcome limitations to the cryopreservation of in vitro produced embryos. DT has been performed by commercial laboratories, ensuring good embryo viability after thawing. However, commercial and operational limitations prevent the large-scale use of these techniques. Thus, this review aims to discuss the use of strategies to improve the postcryopreservation survival capacity and the aspects to be overcome so that the cryopreservation of IVP embryos becomes a well-established and commercially applicable technique in addition to presenting new guidelines for embryo transfer (ET) programs from a better selection of recipients.

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Cryopreservation of Bovine Embryos

Jones

2021

Bovine Reproduction

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A new direct transfer protocol for cryopreserved IVF embryos

Cited by 58 publications

References 20 publications

Effect of single dose follicle stimulating hormone on follicular aspiration, in vitro fertilization and pregnancy rate

Effect of single dose follicle stimulating hormone on follicular aspiration, in vitro fertilization and pregnancy rate

Cryopreservation of in vitro-produced embryos: challenges for commercial implementation

Cryopreservation of Bovine Embryos

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