miR-488 acts as a tumor suppressor gene in gastric cancer

Abstract

Gastric cancer is one of the major causes of cancer mortality. Several microRNAs play a role in the tumor growth and invasion. However, the underlying molecular mechanism remains poorly understood. We detected the miR-638 expression levels in tumor samples and adjacent noncancerous tissues from 68 patients with gastric cancer as well as in the gastric cancer cell line SGC-7901 and SC-M1. The cell cycle was analyzed by flow cytometry, cell proliferation was observed by CCK-8 assay and cell invasion was detected using Transwell assay. MiR-638 was down-regulated in human GC tissues and its expression level was negatively correlated to TNM stage and lymph metastasis. In the cell lines, aberrant expression of miR-638 was related to the cell proliferation, cell cycle and invasion. We also found that SOX2 had a negative correlation with miR-638 in GC tissues, and miR-638 overexpression could decrease SOX2 expression level by directly binding the 3'-UTR of SOX2. in vitro, downregulating SOX2 by siRNA could counteract the effect of miR-638 inhibitor on GC cells proliferation and invasion. Our results demonstrate that miR-638 may play a pivotal role in the growth and invasion of GC.

MiR-488 inhibits proliferation and cisplatin sensibility in non-small-cell lung cancer (NSCLC) cells by activating the eIF3a-mediated NER signaling pathway

“…Moreover, recent studies have found that decreasing eIF3a expression significantly reversed the malignant growth phenotype both in a human lung cancer cell line and in a breast cancer cell line13. An increasing number of studies have shown that miR-488 plays critical roles in several diseases39404142. In prostate carcinoma cells, it functions as tumor suppressor by inhibiting cancer cell proliferation and promoting cancer cell apoptosis39.…”

miR‐384 suppressed renal cell carcinoma cell proliferation and migration through targeting RAB23

“…[16][17][18][19][20] Abnormal or mutated miRNAs have been found to be act as tumor suppressors or oncogenes in various tumors, including gastric cancer, colorectal cancer, hepatocellular carcinoma, tongue squamous cell carcinoma, and osteosarcoma. [21][22][23][24][25][26][27][28] In this study, we showed that miR-384 expression was downregulated in the RCC specimens compared with nontumor specimens. Moreover, overexpression of miR-384 suppressed RCC cell proliferation, cell cycle, and cell migration.…”

MicroRNA‐488‐3p sensitizes malignant melanoma cells to cisplatin by targeting PRKDC

“…In particular, recent studies have suggested a regulatory role of miRNAs in DDP resistance (Ma et al, 2010;Galluzzi et al, 2014). (iii) It has been reported that miR-488-3p functions as tumor suppressors in prostate cancer and gastric cancer (Sikand et al, 2011;Zhao et al, 2016); however, very little was known in MM. (ii) miR-488-3p is a novel miR, and little was known about its function.…”

“…Recently, it has been reported that miR-488-3p is differentially expressed in various types of tissues and plays important roles in the pathogenesis of several diseases (Muinos-Gimeno et al, 2011;Song et al, 2013). For cancer biology, it has been shown that miR-488-3p is downregulated and displays anti-tumor activity in prostate cancer and gastric cancer (Sikand et al, 2011;Zhao et al, 2016). However, the expression patterns and the biological roles of miR-488-3p in MM have not been reported.…”