Interactions Between Monovalent Cations and Nutrient Homeostasis

Abstract

Maintenance of appropriate fluxes of monovalent cation is a requirement for growth and survival. In the budding yeast Saccharomyces cerevisiae an electrochemical gradient of H(+) is fundamental for the uptake of diverse cations, such as K(+), and of many other nutrients. In spite of early work suggesting that alterations in monovalent cation fluxes impact on the uptake and utilization of nutrients, such as phosphate anions, only recently this important aspect of the yeast physiology has been addressed and characterized in some detail. This chapter provides a historical background and summarizes the latest findings.

Transcriptome profile of yeast reveals the essential role of PMA2 and uncharacterized gene YBR056W-A (MNC1) in adaptation to toxic manganese concentration

“…For example, magnesium fluxes across the plasma membrane regulate timekeeping and energy balance 1 and potassium and sodium gradients are essential for cell viability. 2 Yeast is an exclusive model to study the adaptation of eukaryotic cells to toxic heavy metals. [3][4][5][6] Multiple signaling pathways were found to be responsible for yeast viability under an excess of heavy metals.…”

Multi-omics characterization of the necrotrophic mycoparasite Saccharomycopsis schoenii

“…albicans gene nomenclature [54] (Fig 4B). During Nutrient limitation conditions, GO categories such as carnitine and acetyl-CoA metabolism ( CTN3 , CAT2 and CRC1 ), anion and cation transport ( DAL8 , VHT1 and FGR2 ) and the Golgi apparatus ( SGA1 , GAP2 , VHT1 ) were highly enriched, suggesting a focus on peroxisome energy generation and membrane transport facilitation [59, 60]. During Methionine deprivation, membrane transport ( DAL7 , YCT1 , TNA1 , FEN2 , SEO1 , GPT1 , C1_10710C , MUP1 , HGT19 , TPO3 , DUR4 and JEN2 ) GO terms were highly enriched, together with methionine biosynthesis ( CYS1 , MET17 , MET2 and CYS3 ) and oxidation-reduction processes ( C1_01190C , ADI1 , MXR1 , C2_01540W , ADH4 , C2_09850C , GRE3 , ADH6 , PRX1 and C7 _ 03350C ) suggesting, in concert, extensive scavenging and salvaging efforts of methionine and other sulfur compounds (Fig 4B).…”

The role of ion homeostasis in adaptation and tolerance to acetic acid stress in yeasts

“…Pma1 is the main modulator of cytosolic pH (Serrano et al 1986 , Carmelo et al 1997 ). Intracellular pH homeostasis is achieved through the continuous regulation of both cytosolic and organellar pH levels (Canadell and Ariño 2016 ). The pH within any given organelle is determined by a complex interplay involving passive proton leaks alongside the function of various pumps and channels (Brett et al 2005 , Kane 2016 , Ariño et al 2019 ).…”

“…Central to yeast’s stress response is the maintenance of ionic homeostasis, where dissimilarities in ion concentrations across cellular membranes establish crucial gradients for proper cellular function (Orij et al 2011 , Ke et al 2013 , Yenush 2016 , Antunes et al 2023 ). These ionic gradients, which define the transmembrane electrochemical potential, are fundamental for maintaining cellular physiology, solute translocation, and energy homeostasis, preventing toxicity from intracellular surplus ion concentrations (Canadell and Ariño 2016 ). Perturbations to any of these gradients, especially under stress, can substantially disrupt the overall electrochemical transmembrane potential and the balance of other ions, underscoring the interconnectedness of ion homeostasis, cellular function, and stress tolerance (Orij et al 2011 ).…”