Isopropanol biodegradation by immobilized Paracoccus denitrificans in a three-phase fluidized bed reactor

Abstract

A three-phase bed bioreactor including a mix of immobilized microbes was used to degrade isopropanol (IPA). The immobilization method was studied and cells immobilized with calcium alginate, polyvinyl alcohol, activated carbon, and SiO were demonstrated to be the best immobilization method for the degradation of 90% of 2 g/L IPA in just 4 days, 1 day earlier than with free cells. Acetone was monitored as an indicator of microbial IPA utilization as the major intermediate of aerobic IPA biodegradation. The bioreactor was operated at hydraulic retention time (HRT) values of 32, 24, 16, 12, and 10 hr, which correspond to membrane fluxes of 0.03, 0.04, 0.06, 0.08, and 0.10 L/m/hr, respectively. The chemical oxygen demand (COD) removal efficiencies were maintained at 98.0, 97.8, 89.1, 80.6, and 71.1% at a HRT of 32, 24, 16, 12, and 10 hr, respectively, while the IPA degradations were 98.6, 98.3, 90.3, 81.6, and 73.3%, respectively. With a comprehensive consideration of COD removal and economy, the optimal HRT was 24 hr. The results demonstrate the potential of immobilized mixed bacterial consortium in a three-phase fluidized bed reactor system for the aerobic treatment of wastewater containing IPA.

Enhanced Carbofuran Degradation Using Immobilized and Free Cells of Enterobacter sp. Isolated from Soil

“…Bacterial cells were entrapped in gellan gum following an earlier described method by Ahmed et al [ 24 , 51 ]. A culture was grown on a large-scale to obtain enough cells of the strain to be immobilized.…”

Hydrophobic Deep Eutectic Solvents as Greener Substitutes for Conventional Extraction Media: Examples and Techniques

“…Apart from the already mentioned micropollutants, isopropanol compounds are another prevalent toxic component of wastewater that has been widely disposed of by various cosmetic, rubber, etc. industries into watersources. , A study on the separation of isopropanol from aqueous solution was reported using liquid–liquid extraction with the help of two HDESs, namely, 1-decanol:methlytrioctylammonim chloride (2:1) and 1-hexanol:methyltrioctylammonium chloride (2:1) . The distribution coefficient and separation factor for isopropyl alcohol extraction were found to be in the ranges of 1.38–4.13 and 2.50–23.02, respectively, which indicated that the two HDESs were effective at removing isopropyl alcohol from wastewater.…”