PEDF attenuates insulin-dependent molecular pathways of glucose homeostasis in skeletal myocytes

Carnagarin, Revathy; Dharmarajan, Arun; Dass, Crispin R.

doi:10.1016/j.mce.2015.12.010

Cited by 17 publications

(15 citation statements)

References 41 publications

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“…The insulin-dependent signaling pathways in skeletal muscle are upregulated via phosphotidylinositol-3-kinase which phosphorylates Akt at threonine 308, but only partially activates Akt and at serine 473 which fully activates Akt, thereby initiating events important for glucose uptake and metabolism [49, 50]. The results of the current study show that following glucose challenge (increased insulin), the ratio of the levels of skeletal muscle pAKT ser to total AKT was significantly increased in 12–14 year-old offspring untreated or treated with letrozole, indicating that this key component of insulin signaling in skeletal muscle was responsive to insulin in both groups of baboons.…”

Section: Discussionmentioning

confidence: 99%

Insulin resistance elicited in postpubertal primate offspring deprived of estrogen in utero

et al. 2016

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We recently demonstrated that offspring delivered to baboons deprived of estrogen during the second half of gestation exhibited insulin resistance prior to onset of puberty. Because gonadal hormones have a profound effect on insulin action and secretion in adults, we determined whether insulin resistance is retained after initiation of gonadal secretion of testosterone and estradiol. Glucose tolerance tests were performed in postpubertal baboon offspring of untreated and letrozole-treated animals (serum estradiol reduced >95 %). Basal fasting levels of insulin (P < 0.05) and peak 1 min and 1 + 3 + 5 min levels of glucose after glucose tolerance tests challenge (P < 0.03) were greater in offspring delivered to letrozole-treated, estrogen-deprived baboons than untreated animals. Moreover, the value for the HOMA-IR, an accepted index of insulin resistance, was 2-fold greater (P < 0.05) in offspring delivered to baboons treated with letrozole than in untreated animals. Collectively these results support the proposal that estrogen normally has an important role in programming mechanisms in utero within the developing fetus that lead to insulin sensitivity after birth.

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Section: Discussionmentioning

confidence: 99%

Insulin resistance elicited in postpubertal primate offspring deprived of estrogen in utero

et al. 2016

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“…As a member of the serine protease inhibitor family, PEDF has a high a nity to type I collagen in bone tissue. The high expression of PEDF in osteoblasts and active areas of bone formation suggested that it played an important role in the process of bone angiogenesis and matrix reconstruction [24] . For SERPINF1 (serpin peptidase inhibitor, clade F, member 1), located on chromosome 17p13.3, it encodes pigment-epithelium-derived factor (PEDF) [25] .…”

Section: Discussionmentioning

confidence: 99%

Exploring key gene in Acute myeloid leukemia with weighted genes co-expression network analysis

Zhang

Gao

Jia

et al. 2022

Preprint

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Purpose: This study applied the WGCNA method to calculate the data of AML acquired from the TCGA database to discover the genes that are highly related to AML, and figure out key genes from these highly expressed genes, providing novel potential targets and biomarkers for therapy.Methods: The TCGA-AML was downloaded to construct gene co-expression module according to the correlation of genes, and the most significant module exhibiting the highest correlation coefficiency with clinical characteristics was selected. The Cox proportional hazards model was selected for gene survival analysis to screen risk factors related to AML death. Time-dependent ROC was analyzed to screen the genes with the largest ROC curve area and identified as the key genes, and Cox model forest and Nomogram method were constructed to verify the prognosis model.Results: According to the correlation of gene expression, 22 co-expression modules were found. The Grey60 module was significantly positively correlated with clinical characteristics (Cor=0.27, P<0.001). PTCRA, SLC4A3, SERPINF1, PPM1, and MB were screened by survival analysis. Furthermore, PPM1J with the largest AUC area was selected as the key gene. The death risk of the PPM1J high expression group was 2.83 times higher than that of the PPM1J low expression group. The survival rates of patients with different ages and cytogenetic risk types were statistically significantly different (P <0.05). Nomogram displayed that the expression of PPM1J in patients has a great impact on the survival time. The predicted 1-year, 3-year, and 5-year survival rates of patients in the low-expression group of PPM1J are >85%, >85%, and >70%, respectively, while the 1-year, 3-year, and 5-year survival rates of patients in the high-expression group are significantly decreased to 65%, 35%, and 20%, respectively.Conclusion: In this study, the PPM1J gene was screened out by constructing a gene co-expression network, which may become a potential genetic biomarker, affecting the occurrence and prognosis of AML.

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“…Effects of the insulin that was released from the tablets on C2C12 myoblast cells were examined by Glut‐4 translocation. An immunofluorescence experiment was performed using an in‐house protocol on cells seeded in 96‐well plates . Before fixation with 4% paraformaldehyde and fluorescence immunocytochemistry with Glut‐4 antibody (Sigma‐Aldrich), C2C12 cells were treated with placebo tablet, insulin native powder, coated tablets and uncoated tablets for 15 min at room temperature.…”

Section: Methodsmentioning

confidence: 99%

In-vitro evaluation of enteric coated insulin tablets containing absorption enhancer and enzyme inhibitor

Wong

Martinez

Carnagarin

et al. 2017

Journal of Pharmacy and Pharmacology

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PEDF attenuates insulin-dependent molecular pathways of glucose homeostasis in skeletal myocytes

Cited by 17 publications

References 41 publications

Insulin resistance elicited in postpubertal primate offspring deprived of estrogen in utero

Insulin resistance elicited in postpubertal primate offspring deprived of estrogen in utero

Exploring key gene in Acute myeloid leukemia with weighted genes co-expression network analysis

In-vitro evaluation of enteric coated insulin tablets containing absorption enhancer and enzyme inhibitor

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