Alternative splicing transcription of Megalobrama amblycephala HIF prolyl hydroxylase PHD3 and up-regulation of PHD3 by HIF-1α

Chen, Nan; Huang, Chao; Chen, Boxiang; Liu, Hong; Wang, Weimin; Gul, Yasmeen; Wang, Huanling

doi:10.1016/j.bbrc.2015.12.046

Cited by 11 publications

(7 citation statements)

References 35 publications

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“…Igfbp-1 gene regulates the production of reactive oxygen species (ROS) in oxidative stress. Up-regulation of these genes were also observed in freshwater fish [15,32]. In addition, the gimap4 and gimap7 genes were significantly differentially expressed under hypoxia, and similar results were also observed in other fish [33,34].…”

Section: Degs As Adaptive Response To Hypoxiasupporting

confidence: 77%

Comparative Transcriptome Analysis of Gill Tissue in Response to Hypoxia in Silver Sillago (Sillago sihama)

Saetan

Tian

et al. 2020

Animals

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Simple Summary: Silver sillago (Sillago sihama) is a marine fish species with a high economic value. S. sihama is poorly resistant to hypoxia. However, hypoxia stress-related genes and pathways in S. sihama remain unclear. In this study, we compared gill tissues of S. sihama between hypoxia and normoxia groups and detected differentially expressed genes under hypoxia stress. Two gene families, such as cytochrome P450 and glutathione S-transferase were associated with the function of metabolic process under the hypoxia stress. This study will expand our knowledge about the molecular mechanism of the transcriptome response to hypoxia stress in S. sihama.Abstract: Silver sillago (Sillago sihama) is a commercially important marine fish species in East Asia. In this study, we compared the transcriptome response to hypoxia stress in the gill tissue of S. sihama. The fish were divided into four groups, such as 1 h of hypoxia (hypoxia1h, DO = 1.5 ± 0.1 mg/L), 4 h of hypoxia (hypoxia4h, DO = 1.5 ± 0.1 mg/L), 4 h of reoxygen (reoxygen4h, DO = 8.0 ± 0.2 mg/L) after 4 h of hypoxia (DO = 1.5 mg/L), and normoxia or control (DO = 8.0 ± 0.2 mg/L) groups. Compared to the normoxia group, a total of 3550 genes were identified as differentially expressed genes (DEGs) (log 2 foldchange > 1 and padj < 0.05), including 1103, 1451 and 996 genes in hypoxia1h, hypoxia4h and reoxygen4h groups, respectively. Only 247 DEGs were differentially co-expressed in all treatment groups. According to Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, DEGs were significantly enriched in steroid biosynthesis, biosynthesis of amino acids, glutathione metabolism and metabolism of xenobiotics by cytochrome P450, ferroptosis and drug metabolism-cytochrome P450 pathways. Of these, the cytochrome P450 (CYP) and glutathione S-transferase (GST) gene families were widely expressed. Our study represents the insights into the underlying molecular mechanisms of hypoxia stress.Animals 2020, 10, 628 2 of 13 the aquatic environment, which can be accelerated by several factors, such as human activities, water pollution, and intensive fish farming [1]. To adapt to the hypoxic environment, fish produce a range of adaptive physiological mechanisms, such as a rapid change in cell metabolism using ATP [2], regulation of respiratory function [3], floating head [4], and neurological, immune, and hormonal responses [5]. Severe hypoxia can even affect fish reproduction, survival, and cell metabolism [6]. The fish gill is the primary organ for physiological exchanges with the surrounding environment [7]. The fish gill plays a dominant role in aquatic gas exchange and is capable of extensive remodeling in response to changes in the DO level [8]. Many fish respond to hypoxia by increasing the functional surface area of their gill [4]. When the fish returns to normoxic water, the hypoxia-induced gill remodeling is reversed due to the embedding of gill lamellae [9].The sillago silver (Sillago sihama) is a popular species of the family Sillaginidae [10]. S. sihama is ...

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Section: Degs As Adaptive Response To Hypoxiasupporting

confidence: 77%

Comparative Transcriptome Analysis of Gill Tissue in Response to Hypoxia in Silver Sillago (Sillago sihama)

Saetan

Tian

et al. 2020

Animals

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“…The HmP HD2 mRNA was highly expressed in muscle, heart, gill, and intestine, while the HmP HD3 mRNA was highly expressed in liver, heart, muscle, and intestine. This was consistent with the results from M. amblycephala , in which the expression level of MaP HD2 was the highest in muscle [ 23 ] and lower in other tissues, while MaP HD3 [ 24 ] was highly expressed in the liver. However, our data were inconsistent with the results that PHD2 and PHD3 had the highest expression in heart, followed by brain and kidney, in mammals [ 40 ].…”

Section: Discussionsupporting

confidence: 92%

“…This was consistent with the results on M. amblycephala . During hypoxic exposure, the expression levels of MaP HD2 [ 21 ] and MaP HD3 [ 24 ], were significantly increased in the liver and brain. In the heart, gill, and muscle, the expression of HmP HD2 decreased significantly, while the expression of HmP HD3 increased significantly.…”

Section: Discussionmentioning

confidence: 99%

“…PHD 2 was cloned and identified from Megalobrama amblycephala [ 21 ] and Sillago sihama [ 22 ], respectively, and the results showed that the expression patterns of PHD2 were different in these two species under hypoxic conditions. Moreover, the Ma PHD 1 and Ma PHD 3 were also cloned and identified [ 23 , 24 ]. The Ma PHD 1 encodes two protein isoforms generated by alternative initiation, and both isoforms were distributed in the nucleus, therein the senior one promoted cell proliferation [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

“…Ma PHD 3 has two transcripts generated by alternative splicing, and both transcripts were up-regulated under hypoxia. Further evidence is needed to determine whether PHDs play different regulatory roles under hypoxia in different fish species [ 24 ]. Hypophthalmichthys molitrix , one of the most important aquaculture fish species in China, shows poor environmental hypoxia tolerance, making it hard to adapt to the hypoxic environment [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

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Molecular Characterization and Response of Prolyl Hydroxylase Domain (PHD) Genes to Hypoxia Stress in Hypophthalmichthys molitrix

Zhang

Chen

et al. 2022

Animals

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As an economically and ecologically important freshwater fish, silver carp (Hypophthalmichthys molitrix) is sensitive to low oxygen tension. Prolyl hydroxylase domain (PHD) proteins are critical regulators of adaptive responses to hypoxia for their function of regulating the hypoxia inducible factor-1 alpha subunit (HIF-1α) stability via hydroxylation reaction. In the present study, three PHD genes were cloned from H. molitrix by rapid amplification of cDNA ends (RACE). The total length of HmPHD1, HmPHD2, and HmPHD3 were 2981, 1954, and 1847 base pair (bp), and contained 1449, 1080, and 738 bp open reading frames (ORFs) that encoded 482, 359, and 245 amino acids (aa), respectively. Amino acid sequence analysis showed that HmPHD1, HmPHD2, and HmPHD3 had the conserved prolyl 4-hydroxylase alpha subunit homolog domains at their C-termini. Meanwhile, the evaluation of phylogeny revealed PHD2 and PHD3 of H. molitrix were more closely related as they belonged to sister clades, whereas the clade of PHD1 was relatively distant from these two. The transcripts of PHD genes are ubiquitously distributed in H. molitrix tissues, with the highest expressional level of HmPHD1 and HmPHD3 in liver, and HmPHD2 in muscle. After acute hypoxic treatment for 0.5 h, PHD genes of H. molitrix were induced mainly in liver and brain, and different from HmPHD1 and HmPHD2, the expression of HmPHD3 showed no overt tissue specificity. Furthermore, under continued hypoxic condition, PHD genes exhibited an obviously rapid but gradually attenuated response from 3 h to 24 h, and upon reoxygenation, the transcriptional expression of PHD genes showed a decreasing trend in most of the tissues. These results indicate that the PHD genes of H. molitrix are involved in the early response to hypoxic stress, and they show tissue-specific transcript expression when performing physiological regulation functions. This study is of great relevance for advancing our understanding of how PHD genes are regulated when addressing the hypoxic challenge and provides a reference for the subsequent research of the molecular mechanisms underlying hypoxia adaptation in silver carp.

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Molecular response and association analysis of Megalobrama amblycephala fih-1 with hypoxia

et al. 2016

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Hypoxia is one of the most important environmental factors which affect fish growth, development and survival, but regulation mechanisms of hypoxia in fish remain unclear. Therefore, to further understand molecular functions of factor inhibiting HIF-1 (Fih-1), an essential hypoxia sensor, the full-length cDNA of fih-1 was cloned from Megalobrama amblycephala, a hypoxia-sensitive cyprinid fish. The deduced amino acid sequence showed high homology with that of other vertebrates, and all structural and functional domains were highly conserved. The mRNA level in different tissues and developmental stages indicated that M. amblycephala fih-1 expression was higher in liver and muscle, followed by gill, intestine and spleen. During embryogenesis, the fih-1 mRNA was highly expressed in the early embryonic development, then decreased to a very low level, and maintained a relative high level of expression after hatching. In most tissues, the fih-1 mRNA was down-regulated at 2 h but up-regulated at 4 h after hypoxia treatment. In addition, the promoter sequence of M. amblycephala fih-1 was obtained using thermal asymmetric interlaced PCR. Three single nucleotide polymorphism (SNP) sites were found in the cDNA and promoter sequences, and identified significant association with hypoxia trait by correlation analysis in hypoxia-sensitive group and hypoxia-tolerant group. These results demonstrated that M. amblycephala fih-1 plays important roles in embryo development and hypoxia response, which will contribute to systematic understanding of the molecular mechanisms of fish in response to hypoxia, and provide help for fish genetic breeding with hypoxia-tolerant strains or breeds.

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Alternative splicing transcription of Megalobrama amblycephala HIF prolyl hydroxylase PHD3 and up-regulation of PHD3 by HIF-1α

Cited by 11 publications

References 35 publications

Comparative Transcriptome Analysis of Gill Tissue in Response to Hypoxia in Silver Sillago (Sillago sihama)

Comparative Transcriptome Analysis of Gill Tissue in Response to Hypoxia in Silver Sillago (Sillago sihama)

Molecular Characterization and Response of Prolyl Hydroxylase Domain (PHD) Genes to Hypoxia Stress in Hypophthalmichthys molitrix

Molecular response and association analysis of Megalobrama amblycephala fih-1 with hypoxia

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