ADP-Ribosylation Factor 1 Regulates Proliferation, Migration, and Fusion in Early Stage of Osteoclast Differentiation

Kim, Min Jae; Kim, Hyunsoo; Lee, Seoung Hoon; Gu, Dong Ryun; Lee, Soo Young; Lee, Kyung‐Hee; Jeong, Daewon

doi:10.3390/ijms161226168

Cited by 7 publications

(4 citation statements)

References 33 publications

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“…Adherence and migration to the bone surface play essential roles during early stage of osteoclast differentiation ( Kim et al, 2015 ). Thus, we assessed the effect of ELMO1 on adhesion and migration in osteoclast precursors.…”

Section: Resultsmentioning

confidence: 99%

ELMO1 Regulates RANKL-Stimulated Differentiation and Bone Resorption of Osteoclasts

Liang

Hou

et al. 2021

Front. Cell Dev. Biol.

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Bone homeostasis is a metabolic balance between the new bone formation by osteoblasts and old bone resorption by osteoclasts. Excessive osteoclastic bone resorption results in low bone mass, which is the major cause of bone diseases such as rheumatoid arthritis. Small GTPases Rac1 is a key regulator of osteoclast differentiation, but its exact mechanism is not fully understood. ELMO and DOCK proteins form complexes that function as guanine nucleotide exchange factors for Rac activation. Here, we report that ELMO1 plays an important role in differentiation and bone resorption of osteoclasts. Osteoclast precursors derived from bone marrow monocytes (BMMs) of Elmo1–/– mice display defective adhesion and migration during differentiation. The cells also have a reduced activation of Rac1, p38, JNK, and AKT in response to RANKL stimulation. Importantly, we show that bone erosion is alleviated in Elmo1–/– mice in a rheumatoid arthritis mouse model. Taken together, our results suggest that ELMO1, as a regulator of Rac1, regulates osteoclast differentiation and bone resorption both in vitro and in vivo.

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Section: Resultsmentioning

confidence: 99%

ELMO1 Regulates RANKL-Stimulated Differentiation and Bone Resorption of Osteoclasts

Liang

Hou

et al. 2021

Front. Cell Dev. Biol.

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“…For instance, ADP‐ribosylation factor 1 (Arf1) regulates asymmetric cell division in mouse oocyte meiosis through the MAPK signaling pathway (Wang et al, 2009). In addition, human Arf1 expression is positively correlated with cell proliferation (Xu et al, 2017), and mouse Arf1 regulates proliferation in the early stage of mouse osteoclast differentiation (Kim et al, 2015). In summary, ZmArf2 is highly conserved, and it may enlarge plant tissues by promoting cell proliferation.…”

Section: Discussionmentioning

confidence: 99%

An ARF24‐ZmArf2 module influences kernel size in different maize haplotypes

Gao

Zhang

Haonan

et al. 2023

JIPB

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Members of the ADP‐ribosylation factor family, which are GTP‐binding proteins, are involved in metabolite transport, cell division, and expansion. Although there has been a significant amount of research on small GTP‐binding proteins, their roles and functions in regulating maize kernel size remain elusive. Here, we identified ZmArf2 as a maize ADP‐ribosylation factor‐like family member that is highly conserved during evolution. Maize zmarf2 mutants showed a characteristic smaller kernel size. Conversely, ZmArf2 overexpression increased maize kernel size. Furthermore, heterologous expression of ZmArf2 dramatically elevated Arabidopsis and yeast growth by promoting cell division. Using expression quantitative trait loci (eQTL) analysis, we determined that ZmArf2 expression levels in various lines were mainly associated with variation at the gene locus. The promoters of ZmArf2 genes could be divided into two types, pS and pL, that were significantly associated with both ZmArf2 expression levels and kernel size. In yeast‐one‐hybrid screening, maize Auxin Response Factor 24 (ARF24) is directly bound to the ZmArf2 promoter region and negatively regulated ZmArf2 expression. Notably, the pS and pL promoter types each contained an ARF24 binding element: an auxin response element (AuxRE) in pS and an auxin response region (AuxRR) in pL, respectively. ARF24 binding affinity to AuxRR was much higher compared with AuxRE. Overall, our results establish that the small G‐protein ZmArf2 positively regulates maize kernel size and reveals the mechanism of its expression regulation.

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“…Fourth, ADPribosylation factor 1 is a GTP-binding protein that functions as an allosteric activator of ADP-ribosyltransferase. is multifunctional protein can regulate cell proliferation, migration, and fusion [55]. Finally, Cytochrome P450 (CYP) is the sole enzyme responsible for the production of aldosterone in humans [56] and participates in the metabolism of therapeutic drugs, fatty acids, eicosanoids, sterols, steroids, Figure 3: Compound-target networks.…”

Section: Compounds In Ree Tcm Formulae Have Multiple Targetsmentioning

confidence: 99%

Chinese Medicine for Psoriasis Vulgaris Based on Syndrome Pattern: A Network Pharmacological Study

Wang

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Background. The long-term use of conventional therapy for psoriasis vulgaris remains a challenge due to limited or no patient response and severe side effects. Complementary and alternative treatments such as traditional Chinese medicine (TCM) are widely used in East Asia. TCM treatment is based on individual syndrome types. Three TCM formulae, Compound Qingdai Pills (F1), Yujin Yinxie Tablets (F2), and Xiaoyin Tablets (F3), are used for blood heat, blood stasis, and blood dryness type of psoriasis vulgaris, respectively. Objectives. To explore the mechanism of three TCM formulae for three syndrome types of psoriasis vulgaris. Methods. The compounds of the three TCM formulae were retrieved from the Psoriasis Database of Traditional Chinese Medicine (PDTCM). Their molecular properties of absorption, distribution, metabolism, excretion and toxicity (ADME/T), and drug-likeness were compared by analyzing the distribution of compounds in the chemical space. The cellular targets of the compounds were predicted by molecular docking. By constructing the compound-target network and analyzing network centrality, key targets and compounds for each formula were screened. Three syndrome types of psoriasis vulgaris related pathways and biological processes (BPs) were enriched by the Database for Annotation, Visualization, and Integrated Discovery (DAVID) v6.8. Results. The compounds of the three formulae exhibited structural diversity, good drug-like properties, and ADME/T properties. A total of 72, 97 and 85 targets were found to have interactions with compounds of F1, F2, and F3, respectively. The three formulae were all related to 53 targets, 8 pathways, 9 biological processes, and 10 molecular functions (MFs). In addition, each formula had unique targets and regulated different pathways and BPs. Conclusion. The three TCM formulae exhibited common mechanisms to some extent. The differences at molecular and systems levels may contribute to their unique applications in individualized treatment.

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ADP-Ribosylation Factor 1 Regulates Proliferation, Migration, and Fusion in Early Stage of Osteoclast Differentiation

Cited by 7 publications

References 33 publications

ELMO1 Regulates RANKL-Stimulated Differentiation and Bone Resorption of Osteoclasts

ELMO1 Regulates RANKL-Stimulated Differentiation and Bone Resorption of Osteoclasts

An ARF24‐ZmArf2 module influences kernel size in different maize haplotypes

Chinese Medicine for Psoriasis Vulgaris Based on Syndrome Pattern: A Network Pharmacological Study

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