Feel the force: Podosomes in mechanosensing

Linder, Stefan; Wiesner, Christiane

doi:10.1016/j.yexcr.2015.11.026

Cited by 60 publications

(53 citation statements)

References 52 publications

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“…Several studies have revealed the existence of a podosome substructure, the so‐called “cap structure,” situated on top of the podosome (Bhuwania et al, ; Linder & Wiesner, ; Mersich, Miller, Chkourko, & Blystone, ). These cap structures overlaid on top of the podosomes were resistant to mechanical shearing.…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have revealed the existence of a podosome substructure, the so-called "cap structure," situated on top of the podosome (Bhuwania et al, 2012;Linder & Wiesner, 2016;Mersich, Miller, Chkourko, & Blystone, 2010) The appearance of these heterogeneous podosome cores may be ascribed to the podosome dynamics in the SZ as described above.…”

Section: Podosome Cap Complexmentioning

confidence: 95%

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Scattered podosomes and podosomes associated with the sealing zone architecture in cultured osteoclasts revealed by cell shearing, quick freezing, and platinum‐replica electron microscopy

Akisaka

Yoshida

2019

Cytoskeleton

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Osteoclasts (OCs) can adhere to a variety of substrate surfaces by highly dynamic actin‐based cytoskeletal structures termed podosomes. This tight attachment is established by a sealing zone (SZ), which is made of interconnected individual podosomes. Compared with scattered podosomes in various cell types, the architecture of the SZ is still unclear. Especially, ultrastructural studies on the details of the cytoskeletal structure of an OC have been challenging, because the high density of filaments in their podosomes obscure visualization of individual filaments. Therefore, to study this organization in more exact detail, we employed shearing open combined with replica electron microscopy. The present study provides several new details of the podosome and SZ structure, which were previously unrecognized: (a) the SZ consists of recognizable podosomes with a dense actin network of interpodosomal regions characterized by multiple layers of crossing, branching and anastomosing actin filament networks; (b) the Arp2/3 complex is distributed throughout the actin network of podosomes and SZ, indicating that actin polymerization is concentrated at these regions; (c) a close spatial relationship between the podosome and the dorsal membrane; and (d) a network of membranous organelles in close proximity to the podosomes in the SZ. Taken together, the present study reveals that a more complicated interpodosomal actin network among neighboring individual podosomes, which is more complicated than previously thought, appears to form the SZ. Indeed, individual podosomes are not an isolated structural unit from other organelles; and, in turn, their dynamism might affect the surrounding interpodosomal cytoskeletons, membranous organelles, and plasma membrane.

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Section: Discussionmentioning

confidence: 99%

Section: Podosome Cap Complexmentioning

confidence: 95%

Scattered podosomes and podosomes associated with the sealing zone architecture in cultured osteoclasts revealed by cell shearing, quick freezing, and platinum‐replica electron microscopy

Akisaka

Yoshida

2019

Cytoskeleton

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“…The ring contains a combination of two subsets of unbranched actin filaments that contain myosin and appear to be the basis for actomyosin contractility, either at individual invadosomes (i.e. the lateral fibres) or between invadosome cores (the connecting cables) (Linder and Wiesner, 2015;Luxenburg et al, 2007;van den Dries et al, 2013) (Fig. 1A).…”

Section: General Features Of Invadosomesmentioning

confidence: 99%

The microenvironment controls invadosome plasticity

Martino

Henriet

Ezzoukhry

et al. 2016

Journal of Cell Science

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Invadosomes are actin-based structures involved in extracellular matrix degradation. Invadosomes is a term that includes podosomes and invadopodia, which decorate normal and tumour cells, respectively. They are mainly organised into dots or rosettes, and podosomes and invadopodia are often compared and contrasted. Various internal or external stimuli have been shown to induce their formation and/or activity. In this Commentary, we address the impact of the microenvironment and the role of matrix receptors on the formation, and dynamic and degradative activities of invadosomes. In particular, we highlight recent findings regarding the role of type I collagen fibrils in inducing the formation of a new linear organisation of invadosomes. We will also discuss invadosome plasticity more generally and emphasise its physio-pathological relevance.

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“…During cellular invasion processes, cells must be able to degrade and remodel the ECM. One way in which cells accomplish this task is through podosomes and invadopodia (sometimes collectively referred to as ‘invadosomes’) , which are dynamic protrusions of the plasma membrane found in both normal (referred to as podosomes) and in cancer cells (referred to as invadopodia) .…”

Section: Introductionmentioning

confidence: 99%

Invadosomes are coming: new insights into function and disease relevance

2017

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Invadopodia and podosomes are discrete, actin-based molecular protrusions that form in cancer cells and normal cells, respectively, in response to diverse signaling pathways and extracellular matrix cues. Although they participate in a host of different cellular processes, they share a common functional theme of controlling pericellular proteolytic activity, which sets them apart from other structures that function in migration and adhesion, including focal adhesions, lamellipodia, and filopodia. In this review, we highlight research that explores the function of these complex structures, including roles for podosomes in embryonic and postnatal development, in angiogenesis and remodeling of the vasculature, in maturation of the postsynaptic membrane, in antigen sampling and recognition, and in cell-cell fusion mechanisms, as well as the involvement of invadopodia at multiple steps of the metastatic cascade, and how all of this may apply in the treatment of human disease states. Finally, we explore recent research that implicates a novel role for exosomes and microvesicles in invadopodiadependent and invadopodia-independent mechanisms of invasion, respectively.

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Feel the force: Podosomes in mechanosensing

Cited by 60 publications

References 52 publications

Scattered podosomes and podosomes associated with the sealing zone architecture in cultured osteoclasts revealed by cell shearing, quick freezing, and platinum‐replica electron microscopy

Scattered podosomes and podosomes associated with the sealing zone architecture in cultured osteoclasts revealed by cell shearing, quick freezing, and platinum‐replica electron microscopy

The microenvironment controls invadosome plasticity

Invadosomes are coming: new insights into function and disease relevance

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