Effect of Cd2+ on muscle type of creatine kinase: Inhibition kinetics integrating computational simulations

Cai, Yan; Lee, Jinhyuk; Wang, Wei; Yang, Jun‐Mo; Qian, Guo‐Ying

doi:10.1016/j.ijbiomac.2015.11.077

Cited by 6 publications

(2 citation statements)

References 27 publications

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“…Previous reports have discussed the biochemical characteristics of PSCK in P. sinensis, mainly focusing on the muscle (PSCK-M) and brain (PSCK-B) types in vitro folding studies [15,16,20,21,29,30]. In this study, we have focused on the mitochondrial type such as PSCK-S and made a comparison between three types of PSCK isozymes.…”

Section: Discussionmentioning

confidence: 99%

“…The crystallographic protein structures for PSCK isozymes have not yet been elucidated. Therefore we constructed the 3D structures by homology modeling according to previous reports [15,16,20,21]. Each PSCK-B, PSCK-M, and PSCK-S protein structure was modeled using homology templates HHsearch [22] to find template proteins, and the 9 or 10 best template sequences were aligned by application of T-Coffee [23] in its fast mode.…”

Section: Computational Docking Simulations and Molecular Dynamics (Md)mentioning

confidence: 99%

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Comparative studies of the expression of creatine kinase isoforms under immune stress in Pelodiscus sinensis

Wang

Lee

et al. 2020

International Journal of Biological Macromolecules

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The expression and localization of different isoforms of creatine kinase in Pelodiscus sinensis (PSCK) were studied to reveal the role of PSCK isozymes (PSCK-B, PSCK-M, PSCK-S) under bacterial infection-induced immunologic stress. The computational molecular dynamics simulations predicted that PSCK-S would mostly possess a kinase function in a structural aspect when compared to PSCK-B and PSCK-M. The assay of biochemical parameters such as total superoxide dismutase (T-SOD), lactate dehydrogenase (LDH), malondialdehyde (MDA), catalase (CAT), and the content of ATP were measured along with total PSCK activity in different tissue samples under bacterial infection. The expression detections of PSCK isozymes in vitro and in vivo were overall well-matched where PSCK isozymes were expressed differently in P. sinensis tissues. The results showed that PSCK-B mostly contributes to the spleen, followed by the liver and myocardium; PSCK-M mostly contributes to the liver, followed by the myocardium and skeletal muscle, while PSCK-S contributes to the spleen and is uniquely expressed in skeletal muscle. Our study suggests that the various alterations of PSCK isozymes in tissues of P. sinensis are prone to defense the bacterial infection and blocking energetic imbalance before severe pathogenesis turned on in P. sinensis.

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Section: Discussionmentioning

confidence: 99%

Section: Computational Docking Simulations and Molecular Dynamics (Md)mentioning

confidence: 99%

Comparative studies of the expression of creatine kinase isoforms under immune stress in Pelodiscus sinensis

Wang

Lee

et al. 2020

International Journal of Biological Macromolecules

Self Cite

View full text Add to dashboard Cite

show abstract

Kinetic analysis of the transphosphorylation with creatine kinase by pressure-assisted capillary electrophoresis/dynamic frontal analysis

2021

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Effect of Cd2+ on tyrosinase: Integration of inhibition kinetics with computational simulation

Yue

Lee

et al. 2017

International Journal of Biological Macromolecules

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Effect of Cd2+ on muscle type of creatine kinase: Inhibition kinetics integrating computational simulations

Cited by 6 publications

References 27 publications

Comparative studies of the expression of creatine kinase isoforms under immune stress in Pelodiscus sinensis

Comparative studies of the expression of creatine kinase isoforms under immune stress in Pelodiscus sinensis

Kinetic analysis of the transphosphorylation with creatine kinase by pressure-assisted capillary electrophoresis/dynamic frontal analysis

Effect of Cd2+ on tyrosinase: Integration of inhibition kinetics with computational simulation

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