Deciphering the molecular adaptation of the king scallop (Pecten maximus) to heat stress using transcriptomics and proteomics

Artigaud, Sébastien; Richard, Joëlle; Thorne, Michael A. S.; Lavaud, Romain; Flye-Sainte-Marie, Jonathan; Jean, Fred; Peck, Lloyd S.; Clark, Melody S.; Pichereau, Vianney

doi:10.1186/s12864-015-2132-x

Cited by 40 publications

(16 citation statements)

References 80 publications

(119 reference statements)

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“…The expression of two putative apoptosis inhibitors was consistently higher in treated animals than in control animals, while the apoptosis inhibitor BIRC7B was significantly up-regulated in AER_24H and AER_72H. BIRC7 has previously been reported to be increasingly transcribed following heat stress in adult P. maximus (Artigaud et al 2015b).…”

Section: Apoptosismentioning

confidence: 84%

“…RNA sequencing has been increasingly exploited in bivalve species (e.g., Bjärnmark et al 2016;Nie et al 2017;Pauletto et al 2017), yet the P. maximus transcriptome has been poorly investigated, and the available transcriptional data were retrieved from adult tissues (e.g., Artigaud et al 2014bArtigaud et al , 2015aArtigaud et al , 2015b. In the present study, a large number of sequences was obtained from great scallop larvae, and a larval scaffold transcriptome with 80 012 contigs was provided; thus, this research significantly increased the available genomic information for this species, especially regarding its sensitive planktonic stage.…”

Section: Discussionmentioning

confidence: 99%

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Understanding the mechanisms involved in the high sensitivity of Pecten maximus larvae to aeration

et al. 2018

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Among reared bivalves, some "novel species", such as the great scallop, Pecten maximus, have experienced more difficulty with routine reproduction due to their high sensitivity to biological, chemical, and physical stress during stages of early development. Working with high larval densities requires the use of aeration systems to provide optimal larval suspension and feed distribution. The high susceptibility of the great scallop to aeration in small-volume systems may impose an important limitation in hatchery-based practices. The present study aimed to investigate the processes impacted by aeration in P. maximus veliger larvae exposed to continuous aeration in small-volume tanks (10 L). Aeration appeared as major stressor that was responsible for early mortality among exposed animals (at 96 h after aeration started, haa) when exposure started 13 days after fertilization. Exposed larvae and controls were collected at 12, 24 and 72 haa, and a total of 18 cDNA libraries, each representing a pool of approximately 7,500 larvae, were sequenced, obtaining 358,817,016 raw reads. RNA-seq data were first used to build a de novo transcriptome assembly, and differential transcript abundance was assessed in exposed and control groups; thus, the molecular mechanisms involved in high sensitivity to aeration were deciphered. More than 2,000 transcripts were differentially expressed between exposed and control larvae across the entire time series (logFC > 1, FDR < 5%). Functional analysis revealed that transcriptional changes in larvae exposed to aeration mainly involved the genes that regulate digestive activity and energy metabolism, immune defense, inflammation, apoptosis, larval growth, and development. The results of this study demonstrate that, overall, aeration affects the feeding capacity and energy metabolism of larvae, with expected consequences on the animal's fitness, including its swimming efficiency. Aeration also triggered immune responses and apoptosis, which then increased through opportunistic infections. Notably, infections may be a consequence of a bacterial bloom triggered by the first mortality events that occurred in the culture. This study provides insights into the interactions between environmental variables and great scallop larvae physiology, and the results may contribute to the development of strategies for improving larval rearing practices and ensuring long-term sustainability of P. maximus aquaculture. Highlights ► Pecten maximus is highly susceptible to mild aeration, a desirable rearing practice yielding optimal larvae and feed distribution. ► Water turbulence reduced lipid and carbohydrate metabolism in scallop larvae, thus resulting in delayed growth and development. ► As a symptom of stress condition, larvae died, exhausted due to continuous swimming and inability to feed. ► These findings may contribute to develop strategies for improving rearing practices and for long-term sustainability of scallop aquaculture.

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Section: Apoptosismentioning

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Understanding the mechanisms involved in the high sensitivity of Pecten maximus larvae to aeration

et al. 2018

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“…Artigaud and colleagues in 2015 50 reported an increase in the metabolic rate of animals, which was strictly related to glycolysis. In mollusks, an upregulation of the glycolytic pathway has been described during thermal acclimation in providing additional energy resources 51,52 .…”

Section: Discussionmentioning

confidence: 96%

Omics approaches for conservation biology research on the bivalve Chamelea gallina

Carducci

Biscotti

Trucchi

et al. 2020

Sci Rep

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The striped venus (Chamelea gallina) is an important economic resource in the Mediterranean Basin; this species has exhibited a strong quantitative decline in the Adriatic Sea. The aim of this work was to provide a comprehensive view of the biological status of C. gallina to elucidate the bioecological characteristics and genetic diversity of wild populations. To the best of our knowledge, this investigation is the first to perform a multidisciplinary study on C. gallina based on two omics approaches integrated with histological, ecotoxicological, and chemical analyses and with the assessment of environmental parameters. The results obtained through RNA sequencing indicated that the striped venus has a notable ability to adapt to different environmental conditions. Moreover, the stock reduction exhibited by this species in the last 2 decades seems not to have negatively affected its genetic diversity. Indeed, the high level of genetic diversity that emerged from our ddRAD dataset analyses is ascribable to the high larval dispersal rate, which might have played a “compensatory role” on local fluctuations, conferring to this species a good adaptive potential to face the environmental perturbations. These findings may facilitate the efforts of conservation biologists to adopt ad hoc management plans for this fishery resource.

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“…Furthermore, GAPDH was recently identified to be down-regulated in a 2-DE based study on thermal acclimation in the scallop Pecten maximus [83]. As well as its established role in the glycolytic pathway, GAPDH also appears to be involved in a diverse range of cellular processes, including as a transcription factor interacting with RNA polymerase II [84], a signal protein via the mTOR pathway [85] and binding to various nucleic acids [86],…”

Section: Accepted Manuscriptmentioning

confidence: 99%

“…suggesting that it is a central node in a network of thermo-regulated genes and proteins and may play a pivotal role in thermal acclimation [83].…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Non-additive effects of ocean acidification in combination with warming on the larval proteome of the Pacific oyster, Crassostrea gigas

Harney

Artigaud

Souchu

et al. 2016

Journal of Proteomics

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Increasing atmospheric CO2 raises sea surface temperatures and results in ocean acidification, two climatic variables known to impact marine organisms. Larvae of calcifying species may be particularly at risk to such changing environmental conditions. The Pacific oyster Crassostrea gigas is ecologically and commercially important, and understanding its ability to acclimate to climate change will help to predict how aquaculture of this species is likely to be impacted. Modest, yet realistic changes in pH and/or temperature may be more informative of how populations will respond to contemporary climate change. We showed that concurrent acidification and warming mitigates the negative effects of pH alone on size of larvae, but proteomic analysis reveals altered patterns of metabolism and an increase in oxidative stress suggesting non-additive effects of the interaction between pH and temperature on protein abundance. Thus, even small changes in climate may influence development, with potential consequences later in life.

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Deciphering the molecular adaptation of the king scallop (Pecten maximus) to heat stress using transcriptomics and proteomics

Cited by 40 publications

References 80 publications

Understanding the mechanisms involved in the high sensitivity of Pecten maximus larvae to aeration

Understanding the mechanisms involved in the high sensitivity of Pecten maximus larvae to aeration

Omics approaches for conservation biology research on the bivalve Chamelea gallina

Non-additive effects of ocean acidification in combination with warming on the larval proteome of the Pacific oyster, Crassostrea gigas

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