Sorbitol treatment extends lifespan and induces the osmotic stress response in Caenorhabditis elegans

Chandler-Brown, Devon; Choi, Haeri; Park, Shirley; Ocampo, Billie; Chen, Shiwen; Le, Anna; Sutphin, George L.; Shamieh, Lara S.; Smith, Erica D.; Kaeberlein, Matt

doi:10.3389/fgene.2015.00316

Cited by 27 publications

(23 citation statements)

References 61 publications

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“…YUCCAs also promote drought resistance, and some FMOs are involved in osmoregulation in worms (85) and fish (88), in addition to producing the osmolyte TMAO in humans (43). Interestingly, there is overlap between the DR and osmotic stress pathways (89,90), and it is plausible that FMOs act at this intersection.…”

Section: Fmos and Aging In Non-mammalian Speciesmentioning

confidence: 99%

Flavin-containing monooxygenases in aging and disease: Emerging roles for ancient enzymes

Rossner

Kaeberlein

Leiser

2017

Journal of Biological Chemistry

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Edited by F. Peter GuengerichFlavin-containing monooxygenases (FMOs) are primarily studied as xenobiotic metabolizing enzymes with a prominent role in drug metabolism. In contrast, endogenous functions and substrates of FMOs are less well understood. A growing body of recent evidence, however, implicates FMOs in aging, several diseases, and metabolic pathways. The evidence suggests an important role for these well-conserved proteins in multiple processes and raises questions about the endogenous substrate(s) and regulation of FMOs. Here, we present an overview of evidence for FMOs' involvement in aging and disease, discussing the biological context and arguing for increased investigation into the function of these enzymes.

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Section: Fmos and Aging In Non-mammalian Speciesmentioning

confidence: 99%

Flavin-containing monooxygenases in aging and disease: Emerging roles for ancient enzymes

Rossner

Kaeberlein

Leiser

2017

Journal of Biological Chemistry

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“…Similarly, sorbitol‐induced hyperosmolarity causes lifespan extension in worms (Chandler‐Brown et al . ) and increases replicative (Kaeberlein et al . ) and chronological lifespan in yeast (Smith et al .…”

Section: Introductionmentioning

confidence: 99%

The role of declining adaptive homeostasis in ageing

Pomatto

Davies

2017

The Journal of Physiology

153

108

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Adaptive homeostasis is "the transient expansion or contraction of the homeostatic range for any given physiological parameter in response to exposure to sub-toxic, non-damaging, signalling molecules or events, or the removal or cessation of such molecules or events" (Davies, 2016). Adaptive homeostasis enables biological systems to make continuous short-term adjustments for optimal functioning despite ever-changing internal and external environments. Initiation of adaptation in response to an appropriate signal allows organisms to successfully cope with much greater, normally toxic, stresses. These short-term responses are initiated following effective signals, including hypoxia, cold shock, heat shock, oxidative stress, exercise-induced adaptation, caloric restriction, osmotic stress, mechanical stress, immune response, and even emotional stress. There is now substantial literature detailing a decline in adaptive homeostasis that, unfortunately, appears to manifest with ageing, especially in the last third of the lifespan. In this review, we present the hypothesis that one hallmark of the ageing process is a significant decline in adaptive homeostasis capacity. We discuss the mechanistic importance of diminished capacity for short-term (reversible) adaptive responses (both biochemical and signal transduction/gene expression-based) to changing internal and external conditions, for short-term survival and for lifespan and healthspan. Studies of cultured mammalian cells, worms, flies, rodents, simians, apes, and even humans, all indicate declining adaptive homeostasis as a potential contributor to age-dependent senescence, increased risk of disease, and even mortality. Emerging work points to Nrf2-Keap1 signal transduction pathway inhibitors, including Bach1 and c-Myc, both of whose tissue concentrations increase with age, as possible major causes for age-dependent loss of adaptive homeostasis.

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“…32,40 We therefore examined the total time of larval development, from hatching to the molting event that marks the transition from the fourth larval stage to adulthood. We compared our individual culture system to bulk culture in petri dishes, using 1.7% agar with corral-NGM.…”

Section: Resultsmentioning

confidence: 99%

A simple culture system for long-term imaging of individual C. elegans

et al. 2017

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We have miniaturized standard culture techniques to rear arrays of isolated, individual C. elegans throughout their lives on solid gel media. The resulting apparatus is compatible with brightfield and fluorescent microscopy, enabling longitudinal studies of morphology and fluorescent transgene expression. Our culture system exploits a novel crosslinking reaction between a polyethylene glycol hydrogel and a silicone elastomer to constrain animals to individual “corrals” on the gel surface. These devices are simple to construct on the benchtop with commercially available reagents, and, unlike microfluidic isolation methods, does not rely on micropatterned materials. We demonstrate that this new culture method has negligible effects on the physiology of C. elegans compared to standard culture on agar plates. In addition, RNAi techniques are effective in this system. Finally, the hydrogel–silicone binding chemistry that we developed also allows traditional microfluidic devices to be covalently attached to gel substrates instead of glass.

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Sorbitol treatment extends lifespan and induces the osmotic stress response in Caenorhabditis elegans

Cited by 27 publications

References 61 publications

Flavin-containing monooxygenases in aging and disease: Emerging roles for ancient enzymes

Flavin-containing monooxygenases in aging and disease: Emerging roles for ancient enzymes

The role of declining adaptive homeostasis in ageing

A simple culture system for long-term imaging of individual C. elegans

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