A targeted proteomic strategy for the measurement of oral cancer candidate biomarkers in human saliva

Kawahara, Rebeca; Bollinger, James G.; Rivera, César; Ribeiro, Ana Carolina Prado; Brandão, Thaís Bianca; Leme, Adriana Franco Paes; MacCoss, Michael J.

doi:10.1002/pmic.201500224

Cited by 69 publications

(54 citation statements)

References 51 publications

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“…The CFH was previously identified in lung adenocarcinoma and cutaneous squamous cell carcinoma, but not in OSCC , and apoB100 was found in serum of patients with head and neck squamous cell carcinoma . No data were found on the presence of complement C5 and mucin‐7 in cancer; however, other components of the complement system and other forms of mucins were all identified in different forms of cancer and in OSCC as well .…”

Section: Resultsmentioning

confidence: 99%

“…Complement C4B (C4B), complement factor B (CFB), complement C3, and alpha‐1‐antitrypsin were shown to be associated with the risk of developing OSCC according to a targeted proteomics study . The levels of apolipoproteins A and E; PON1; inter‐alpha‐trypsin inhibitor heavy chain H1, H2, and H4; kininogen 1; protein AMBP; nucleobindin‐2; SERPIND1; and SERPINA6 were found to be upregulated in OSCC in shotgun proteomics experiments carried out on saliva samples .…”

Section: Resultsmentioning

confidence: 99%

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Salivary proteome profiling of oral squamous cell carcinoma in a Hungarian population

et al. 2018

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Oral squamous cell carcinoma (OSCC) is the seventh most common malignancy and the ninth most frequent cause of cancer death in Europe. Within Europe, Hungary has one of the highest rates of OSCC incidence and mortality. Thus, there is an urgent need to improve early detection. Saliva, as a readily available body fluid, became an increasingly important substance for the detection of biomarkers for many diseases. Different research groups have identified salivary biomarkers specific for OSCC for different countries. In this study, saliva samples of Hungarian patients with OSCC were studied to discover disease‐specific and perhaps region‐specific biomarkers. LC‐mass spectrometry (MS)/MS analysis on a linear ion trap‐Orbitrap mass spectrometer was used for qualitative and quantitative salivary protein profiling. More than 500 proteins were identified from saliva by shotgun proteomics. The up‐ and downregulated proteins in the saliva of patients with OSCC highlighted the importance of protein–protein interaction networks involving the immune system and proteolysis in disease development. Two potential biomarkers from our shotgun analysis and a third candidate reported earlier by a Taiwanese group were further examined by ELISA on a larger reference set of samples. Resistin, a biomarker reported in Taiwan but not validated in our study, highlights the necessity of application of standardized analysis methods in different ethnic or geographical populations to identify biomarkers with sufficient specificity and sensitivity.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Salivary proteome profiling of oral squamous cell carcinoma in a Hungarian population

et al. 2018

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“…Compared with other samples, collection of saliva is convenient, non-invasive, economical and not influenced by the menstrual cycle. For this reason, some studies have focused on using saliva to detect diseases and have showed some clinical benefits [10,11]. Similarly, saliva could be used to improve the comprehensiveness of studies on TCM constitution.…”

Section: Introductionmentioning

confidence: 99%

miRNA Expression Profile of Saliva in Subjects of Yang Deficiency Constitution and Yin Deficiency Constitution

Chen

Wu²,

Yao

et al. 2018

Cell Physiol Biochem

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Background/Aims: Based on the theory of constitution in Traditional Chinese Medicine (TCM), the Chinese Han population has been classified into nine constitutions. Of these, Yang deficiency constitution mainly exhibit cold intolerance while Yin deficiency constitution mainly exhibit heat intolerance. Some studies have been carried out to explore the modern genetic and biological basis of such constitution classification, but more remains to be done. MicroRNA (miRNA) serves as post-transcriptional regulators of gene expression and may play a role in the classification process. Here, we examined miRNA expression profile of saliva to further improve the comprehensiveness of constitution classification. Methods: Saliva was collected from Chinese Han individuals with Yang deficiency, Yin deficiency and Balanced constitutions (n=5 each), and miRNA expression profile was determined using the Human miRNA OneArray®v7. Based on 1.5 Fold change, means log₂|Ratio|≥0.585 and P-value< 0.05, differentially expressed miRNA was screened. Target genes were predicted using DIANA-TarBasev7.0 and analysis of KEGG pathway was carried out using DIANA-mirPathv.3. Results: We found that 81 and 98 differentially expressed miRNAs were screened in Yang deficiency and Yin deficiency constitution, respectively. Among them, 16 miRNAs were identical and the others were unique. In addition, the target genes that are regulated by the unique miRNAs were significantly enriched in 27 and 20 signaling pathways in Yang deficiency and Yin deficiency constitution, respectively. Thyroid hormone signaling pathway is present in both constitutions. These unique miRNAs that regulated target genes of thyroid hormone signaling pathway may be associated with cold intolerance or heat intolerance. Conclusion: The results of our study show that Yang deficiency and Yin deficiency constitutions exhibit systematic differences in miRNA expression profile. Moreover, the distinct characteristics of TCM constitution may be explained, in part, by differentially expressed miRNAs.

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“…Between the proteins identified in the discovery phase, there was a significant enrichment of proteins involved in the complement and coagulation activation cascades (C5, C8G, C1QC, C7, and C9), which were previously considered as biomarkers of different cancers, [17] and several apolipoproteins also reported as plasma biomarkers for different types of cancer and obesity-cancer association. Much of the biological variance in cancer patients is not related to the disease but to the different genetic background of the individuals.…”

Section: Discussionmentioning

confidence: 99%

Identification and Validation of Stage‐Associated Serum Biomarkers in Colorectal Cancer Using MS‐Based Procedures

Marín-Vicente

Mendes

Rı́os

et al. 2019

Proteomics Clinical Apps

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Purpose Successful prevention of colorectal cancer (CRC) would benefit from a rapid serum screening for early detection. Here, a novel strategy for CRC biomarker discovery and validation exclusively based on MS procedures is reported. Experimental design Identification of CRC serum biomarkers is initially made using label‐free quantification on pooled serum samples from different CRC stages followed by two consecutive steps of targeted parallel reaction monitoring assays in different serum cohorts. Relevance of different protein depletion and peptide fractionation extent is investigated. Absolute quantification of a selected peptide is performed as a proof‐of‐concept. Results A total of 945 proteins showed differential abundance in the discovery phase. Based on their statistical significance and relative expression in disease stages, 123 potential biomarkers are selected for a training step. In the final validation step, five peptides belonging to four proteins are consistently quantified in individual CRC serum samples and controls. Different statistical analyses indicate that peptides GWVTDGFSSLK (APOC3) and LCNNPTPQFGGK (THBS1) are candidate biomarkers. Absolute quantification of LCNNPTPQFGGK shows statistical significance for the diagnosis of early respect to late CRC stages. Conclusions and clinical relevance Two peptides from APOC3 and THBS1 are validated by PRM as potential biomarkers for non‐invasive diagnosis of colorectal cancer.

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A targeted proteomic strategy for the measurement of oral cancer candidate biomarkers in human saliva

Cited by 69 publications

References 51 publications

Salivary proteome profiling of oral squamous cell carcinoma in a Hungarian population

Salivary proteome profiling of oral squamous cell carcinoma in a Hungarian population

miRNA Expression Profile of Saliva in Subjects of Yang Deficiency Constitution and Yin Deficiency Constitution

Identification and Validation of Stage‐Associated Serum Biomarkers in Colorectal Cancer Using MS‐Based Procedures

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