Coordinated regulation of nitrogen supply mode and initial cell density for energy storage compounds production with economized nitrogen utilization in a marine microalga Isochrysis zhangjiangensis

Chi, Lei; Yao, Changhong; Cao, Xupeng; Xue, Song

doi:10.1016/j.biortech.2015.10.059

Cited by 25 publications

(14 citation statements)

References 34 publications

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“…Carbohydrate analysis was performed by sulfuric acid-anthrone method according to Chi et al (2016). Protein content was measured with Bradford method according to Yao et al (2012).…”

Section: Biochemical Composition Analysismentioning

confidence: 99%

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Evaluation of the integrated hydrothermal carbonization-algal cultivation process for enhanced nitrogen utilization in Arthrospira platensis production

Yao

Pan

et al. 2016

Bioresource Technology

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“…Carbohydrate analysis was performed by sulfuric acid-anthrone method according to Chi et al (2016). Protein content was measured with Bradford method according to Yao et al (2012).…”

Section: Biochemical Composition Analysismentioning

confidence: 99%

“…Biomass yield (dry weight, DW, g/L) was measured according to Chi et al (2016) with minor modification. Briefly the cultures (10 mL) were filtered using pre-weighed Whatman GF/C filters (47 mm diameter), washed twice with 2 mL of distilled water, and dried to a constant weight at 60°C.…”

Section: Biomass Measurementmentioning

confidence: 99%

Evaluation of the integrated hydrothermal carbonization-algal cultivation process for enhanced nitrogen utilization in Arthrospira platensis production

Yao

Pan

et al. 2016

Bioresource Technology

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“…The cells were harvested by centrifugation at 6800 g for 5 min before they were vacuum dried. The dry cell weight (DW, g/L) was measured according to the procedure described by Chi et al 28 Briefly, the cultures (10 mL) were filtered using pre-weighed Whatman GF/C filters (47 mm diameter), washed twice with 2 mL of distilled water, and dried to a constant weight at 60 C. The dry weight of the algal cells was the difference between the final weight and the weight of the clean filter.…”

Section: Culture Conditions and Growth Measurementmentioning

confidence: 99%

“…Carbohydrate analysis was performed by the sulfuric acid-anthrone method as described by Chi et al. 28…”

Section: Carbohydrate Analysismentioning

confidence: 99%

FabG can function as PhaB for poly-3-hydroxybutyrate biosynthesis in photosynthetic cyanobacteria Synechocystis sp. PCC 6803

et al. 2017

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The production of poly-3-hydroxybutyrate (PHB) by photosynthetic cyanobacteria is a potentially sustainable production method for the biodegradable plastics industry. β-Ketoacyl-ACP reductase (FabG), from the photosynthetic cyanobacterium Synechocystis sp. PCC 6803 (SpFabG), is the first NADPH-dependent reductase in the fatty acid biosynthesis pathway. Its structure is similar to that of acetoacetyl-CoA reductase (SpPhaB), which is critical for PHB synthesis and can replace SpPhaB for acetoacetyl-CoA reduction in vitro. However, the specific function of SpFabG in fatty acid synthesis and whether SpFabG could participate in PHB synthesis in vivo were not yet clear. In this study, the role of SpFabG in fatty acid synthesis was first verified in vivo by knocking down and overexpressing of fabG. It was shown that SpFabG was essential yet not rate-limiting for fatty acid biosynthesis. The biochemical characterization of SpFabG using acetoacetyl-CoA as the substrate showed that the optimum temperature, optimum pH, K and k were 30°C, 7, 2.30 mM, and 19.85 s, respectively, which exemplified the ability of SpFabG to reduce acetoacetyl-CoA with a relatively low affinity and weak catalytic efficiency. Functional analysis of SpFabG in vivo indicated that SpFabG was able to partially complement SpPhaB under nitrogen-deprived conditions, and overexpression of fabG led to the diversion of partial carbon flux from fatty acid toward PHB synthesis.

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“…Rattanapoltee & Kaewkannetra (2014) apuntan que el uso de sacarosa en bajas concentraciones para la acumulación de lípidos en Chlorella vulgaris Beijerinck resulta ser el sustrato más económico en relación costo-beneficio. Chi et al (2016) indican que el costo del nitrógeno requerido para el crecimiento microalgal debe ser considerado en los procesos de escalamiento para la producción de biodiésel. Estos autores reportaron un 66.5% de acumulación de lípidos en Isochrysis zhangjiangesis Hu et Liu y una productividad de lípidos de 3.28 g/d por cada gramo de nitrógeno alimentado al cultivo, lo cual consideran un uso eficiente del nitrógeno.…”

Section: Microalgas Como Fuente De Biodiésel: Avances Y Perspectivasunclassified

Biodiesel production from microalgae: progress and biotechnological prospects

et al. 2017

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RESUMENAntecedentes. Las microalgas son una alternativa para la obtención de biodiésel por su alto rendimiento de lípidos y su perfil de ácidos grasos. Objetivos. Hacer una revisión sobre los avances y perspectivas actuales de la producción de biodiésel a partir de microalgas. Métodos. Se realizó una búsqueda actualizada de los trabajos de investigación relacionados con la producción de biodiésel a partir de microalgas, con especial énfasis en la biosíntesis de ácidos grasos y triglicéridos, la producción de biomasa, las técnicas de extracción, los procesos biotecnológicos implementados en sistemas de cultivo, la transesterificación y los sistemas de doble propósito. Resultados. Las microalgas tienen rendimientos altos de producción de lípidos (59 m 3 ha -1 año -1 ), por lo que representa una alternativa para la obtención de biodiésel; sin embargo, el costo de producción y recuperación de biomasa sigue siendo elevado ($5.8 USD Kg -1 ), aunado a los altos requerimientos energéticos (33 MJ Kg -1 ). Actualmente no existen técnicas industriales factibles para la extracción de lípidos y se están probando los métodos por fluidos supercríticos, campo eléctrico de pulso, microondas y ultrasonicación. La biotecnología ha propuesto un novedoso sistema biológico mediante el uso de lipasas recuperadas de hongos filamentosos para el proceso de transesterificación, los cuales ya son comerciales, y ha logrado rendimientos de biocatálisis mayores al 90%. Los "sistema de doble propósito" pueden ser optimizados utilizando un diseño modular que establezca los procesos y operaciones unitarias bien definidas. Conclusiones. El uso de microalgas para la obtención de biodiésel representa una técnica viable gracias a su alto contenido lipídico y a su perfil de ácidos grasos, aunque hace falta el desarrollo de tecnologías que disminuyan el costo de producción. El uso de sistemas de doble propósito se vislumbra como una buena opción para reducir estos precios, al mismo tiempo que se reusan aguas residuales. ABSTRACTBackground. Microalgae have proven to be an excellent alternative to produce biodiesel due of its high lipid yield and its fatty acid profile. Goals. A review was made on the current advances and perspectives on the production of biodiesel from microalgae. Methods. An updated search was done of the research work related to biodiesel production from microalgae, emphasizing fatty acid and triglyceride biosynthesis, biomass production, extraction techniques, biotechnological processes implemented in culture systems, transesterification and dual purpose systems. Results. Microalgae have shown high yields of lipid production (59 m 3 ha -1 year -1 ) representing an alternative to produce biodiesel. However, costs of biomass production and recovery remain high (US $ 5.8 kg -1 ), coupled with high-energy requirements (33 MJ kg-kg -1 ). Supercritical fluids, electric pulse field, microwave and ultra sonication have being tested for lipid extraction, but currently there are no feasible industrial techniques in this field. Biotechnology ha...

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Coordinated regulation of nitrogen supply mode and initial cell density for energy storage compounds production with economized nitrogen utilization in a marine microalga Isochrysis zhangjiangensis

Cited by 25 publications

References 34 publications

Evaluation of the integrated hydrothermal carbonization-algal cultivation process for enhanced nitrogen utilization in Arthrospira platensis production

Evaluation of the integrated hydrothermal carbonization-algal cultivation process for enhanced nitrogen utilization in Arthrospira platensis production

FabG can function as PhaB for poly-3-hydroxybutyrate biosynthesis in photosynthetic cyanobacteria Synechocystis sp. PCC 6803

Biodiesel production from microalgae: progress and biotechnological prospects

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