Molecular mechanism of fluoroquinolones modulation on corneal fibroblast motility

Chen, Tsan‐Chi; Tsai, Tzu-Yun; Chang, Shu‐Wen

doi:10.1016/j.exer.2015.10.018

Cited by 8 publications

(2 citation statements)

References 33 publications

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“…3 B. Consistent with the Cip effect on HDF cells, Chen et al 33 reported that Cip also notably reduced the migration of human corneal fibroblasts. The decrease in the cell migration observed for CB alone or with Cip 80 may have been due to a Cip cytotoxic effect on the cells (see Figs.…”

Section: Resultsmentioning

confidence: 61%

Ionic complexation improves wound healing in deep second-degree burns and reduces in-vitro ciprofloxacin cytotoxicity in fibroblasts

Sanchez

Guzmán

Flores-Martín

et al. 2022

Sci Rep

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The development of new treatments capable of controlling infections and pain related to burns continues to be a challenge. Antimicrobials are necessary tools, but these can be cytotoxic for regenerating cells. In this study, antibiotic-anesthetic (AA) smart systems obtained by ionic complexation of polyelectrolytes with ciprofloxacin and lidocaine were obtained as films and hydrogels. Ionic complexation with sodium alginate and hyaluronate decreased cytotoxicity of ciprofloxacin above 70% in a primary culture of isolated fibroblasts (p < 0.05). In addition, the relative levels of the proteins involved in cell migration, integrin β1 and p-FAK, increased above 1.5 times (p < 0.05) with no significant differences in cell mobility. Evaluation of the systems in a deep second-degree burn model revealed that reepithelization rate was AA-films = AA-hydrogels > control films > no treated > reference cream (silver sulfadiazine cream). In addition, appendage conservation and complete dermis organization were achieved in AA-films and AA-hydrogels. Encouragingly, both the films and the hydrogels showed a significantly superior performance compared to the reference treatment. This work highlights the great potential of this smart system as an attractive dressing for burns, which surpasses currently available treatments.

show abstract

Section: Resultsmentioning

confidence: 61%

Ionic complexation improves wound healing in deep second-degree burns and reduces in-vitro ciprofloxacin cytotoxicity in fibroblasts

Sanchez

Guzmán

Flores-Martín

et al. 2022

Sci Rep

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show abstract

“…Consistent with the Cip effect on HDF cells, Chen et al (2016) 21 reported that Cip also notably reduced the migration of human corneal broblasts. The decrease in the cell migration observed for CB alone or with CIP 80 may have been due to a Cip cytotoxic effect on the cells (see Figures 1 and 2), although an impact of the high viscosity of CB cannot be discarded.…”

Section: Effect Of Pe and Its Pe-cip Complexes On Hdf Cell Migrationmentioning

confidence: 74%

Polyelectrolyte Complexes of Ciprofloxacin and Lidocaine Improve Wound Healing in Deep Second Degree Burns and Reduce in Vitro Ciprofloxacin Cytotoxicity in Fibroblasts

Sanchez

Guzmán

Flores-Martín

et al. 2022

Preprint

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The development of new treatments capable of controlling infections and pain related to burns continues to be a challenge. Antimicrobials are necessary tools, but these can be cytotoxic for regenerating cells.In this study, antibiotic-anesthetic smart systems obtained by ionic complexation of polyelectrolytes with ciprofloxacin and lidocaine were obtained as film and hydrogel. The complexation of ciprofloxacin with natural polyelectrolytes efficiently contributed to increasing biocompatibility in a primary culture of isolated fibroblasts. In addition, the relative levels of the proteins integrin β1 and p-FAK involved in cell migration were increased with no modifications in cell mobility. Their evaluation in a deep second-degree burn model revealed fast reepithelization, with appendage conservation and complete dermis organization. Encouragingly, we found that both the film and the hydrogel showed a significantly superior performance compared to the reference treatment of silver sulfadiazine cream. This work highlights the great potential of this smart system as an attractive dressing for burns, which surpasses currently available treatments.

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Mitomycin C modulates intracellular matrix metalloproteinase-9 expression and affects corneal fibroblast migration

Chen

Lai

et al. 2019

European Journal of Pharmacology

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Molecular mechanism of fluoroquinolones modulation on corneal fibroblast motility

Cited by 8 publications

References 33 publications

Ionic complexation improves wound healing in deep second-degree burns and reduces in-vitro ciprofloxacin cytotoxicity in fibroblasts

Ionic complexation improves wound healing in deep second-degree burns and reduces in-vitro ciprofloxacin cytotoxicity in fibroblasts

Polyelectrolyte Complexes of Ciprofloxacin and Lidocaine Improve Wound Healing in Deep Second Degree Burns and Reduce in Vitro Ciprofloxacin Cytotoxicity in Fibroblasts

Mitomycin C modulates intracellular matrix metalloproteinase-9 expression and affects corneal fibroblast migration

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