Aged garlic extract enhances heme oxygenase-1 and glutamate-cysteine ligase modifier subunit expression via the nuclear factor erythroid 2–related factor 2–antioxidant response element signaling pathway in human endothelial cells

Hiramatsu, Kiyoshi; Tsuneyoshi, Tadamitsu; Ogawa, Takahiro; Morihara, Naoaki

doi:10.1016/j.nutres.2015.09.018

Cited by 45 publications

(27 citation statements)

References 47 publications

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“…The stimulatory effects of Allium sativum extracts on Nrf2 signaling were described previously in a cellular model of cadmium-induced toxicity, in an animal model of chromium toxicity, and in human endothelial cells (Hiramatsu et al, 2015; Kalayarasan et al, 2008; Lawal and Ellis, 2011). In addition, phenolic diterpenes isolated from Salvia officinalis were previously shown to induce activation of Nrf2-dependent transcription in mouse primary cortical cultures (Fischedick et al, 2013).…”

Section: Resultsmentioning

confidence: 99%

Pikuni-Blackfeet traditional medicine: Neuroprotective activities of medicinal plants used to treat Parkinson’s disease-related symptoms

Jacquet

Tambe

Ying

et al. 2017

Journal of Ethnopharmacology

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Ethnopharmacological relevance Parkinson’s disease (PD) is a multifactorial neurodegenerative disorder affecting 5% of the population over the age of 85 years. Current treatments primarily involve dopamine replacement therapy, which leads to temporary relief of motor symptoms but fails to slow the underlying neurodegeneration. Thus, there is a need for safe PD therapies with neuroprotective activity. In this study, we analyzed contemporary herbal medicinal practices used by members of the Pikuni-Blackfeet tribe from Western Montana to treat PD-related symptoms, in an effort to identify medicinal plants that are affordable to traditional communities and accessible to larger populations. Aim of the study The aims of this study were to (i) identify medicinal plants used by the Pikuni-Blackfeet tribe to treat individuals with symptoms related to PD or other CNS disorders, and (ii) characterize a subset of the identified plants in terms of antioxidant and neuroprotective activities in cellular models of PD. Materials and Methods Interviews of healers and local people were carried out on the Blackfeet Indian reservation. Plant samples were collected, and water extracts were produced for subsequent analysis. A subset of botanical extracts was tested for the ability to induce activation of the Nrf2-mediated transcriptional response and to protect against neurotoxicity elicited by the PD-related toxins rotenone and paraquat. Results The ethnopharmacological interviews resulted in the documentation of 19 medicinal plants used to treat various ailments and diseases, including symptoms related to PD. Seven botanical extracts (out of a total of 10 extracts tested) showed activation of Nrf2-mediated transcriptional activity in primary cortical astrocytes. Extracts prepared from Allium sativum cloves, Trifolium pratense flowers, and Amelanchier arborea berries exhibited neuroprotective activity against toxicity elicited by rotenone, whereas only the extracts prepared from Allium sativum and Amelanchier arborea alleviated PQ-induced dopaminergic cell death. Conclusions Our findings highlight the potential clinical utility of plants used for medicinal purposes over generations by the Pikuni-Blackfeet people, and they shed light on mechanisms by which the plant extracts could slow neurodegeneration in PD.

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Section: Resultsmentioning

confidence: 99%

Pikuni-Blackfeet traditional medicine: Neuroprotective activities of medicinal plants used to treat Parkinson’s disease-related symptoms

Jacquet

Tambe

Ying

et al. 2017

Journal of Ethnopharmacology

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“…In addition, garlic (allicin) has antioxidant property, which might be responsible for the antihypertensive effect of allicin by lowering the level of oxidative stress in hypertensive rats, consequently restoring NO–redox imbalance and increasing NO bioavailability 26, 27. Recently, Hiramatsu et al 29 reported that aged garlic extract increases the expression of nuclear factor erythroid 2-related factor 2, which is responsible for the antioxidant activity. They confirmed that aged garlic extract decreases oxidative stress and maintains cellular redox balance via the nuclear factor erythroid 2-related factor 2–antioxidant response element signaling pathway.…”

Section: Discussionmentioning

confidence: 99%

Antihypertensive effect of allicin in dexamethasone-induced hypertensive rats

Dubey

Singh

Patole³

et al. 2017

Integrative Medicine Research

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BackgroundGlucocorticoid is among the most commonly prescribed medicine. Unfortunately, Excess glucocorticoid level leads hypertension in 80–90% patients. Garlic (Allium sativum) has been used since ancient times and even nowadays as a part of popular medicine for various ailments and physiological disorders. Hence this study was undertaken to investigate the antihypertensive activity of allicin in dexamethasone induced hypertension in wistar rats.MethodsThe animals were randomly divided into four groups comprising of six rats per group. Hypertension was induced by subcutaneous injection of dexamethasone (10 μg/rat/day) in hypertensive rats. Two hypertensive group animals were treated with nicorandil (6 mg/kg/day, po) and allicin (8 mg/kg/day, po) respectively for 8 weeks. While systolic blood pressure (SBP) was measured by the tail-cuff method weekly up to 8 weeks.ResultsDexamethasone treatment resulted in significant increase in SBP while allicin treatment significantly decreases the SBP. Thus, this study confirmed that allicin treatment for 8 weeks partially reverse dexamethasone induced hypertension in rats. Allicin treatment also attenuated dexamethasone-induced anorexia and loss of total body weight.ConclusionThis result suggests antihypertensive effects of allicin in dexamethasone induced hypertension. However, further studies are needed to explore the detailed mechanism of antihypertensive effect of allicin.

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“…Aged garlic extract (AGE) and sulfur compounds. AGE was prepared as previously described (12). Briefly, AGE was manufactured according to the following steps: Originally grown raw garlic (A sativum L.) was cut into slices, immersed in aqueous ethanol, and extracted for >10 months at room temperature (12).…”

Section: Methodsmentioning

confidence: 99%

“…AGE was prepared as previously described (12). Briefly, AGE was manufactured according to the following steps: Originally grown raw garlic (A sativum L.) was cut into slices, immersed in aqueous ethanol, and extracted for >10 months at room temperature (12). The sulfur-containing amino acids, S1PC, SAC and SAMC, are produced during the aging process of raw garlic in 50% ethanol solution for >10 months (19).…”

Section: Methodsmentioning

confidence: 99%

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Sulfur‑containing amino acids in aged garlic extract inhibit inflammation in human gingival epithelial cells by suppressing intercellular adhesion molecule‑1 expression and IL‑6 secretion

Ohtani

Nishimura

2019

biom rep

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Aged garlic extract (AGE) contains various biologically active sulfur-containing amino acids, such as S-allylcysteine (SAC), S-1-propenylcysteine (S1PC) and S-allylmercaptocysteine (SAMC). These amino acids have been demonstrated to lower hypertension, improve atherosclerosis and enhance immunity through their anti-inflammatory and antioxidant activities. It was recently reported that the administration of AGE alleviated gingivitis in a clinical trial. In this study, to gain insight into this effect of AGE, the authors examined whether AGE and the three above-mentioned sulfur compounds influence the effects of tumor necrosis factor-α (TNF-α) in inducing intercellular adhesion molecule-1 (ICAM-1) expression and interleukin-6 (IL-6) secretion in Ca9-22 human gingival epithelial cells. It was found that S1PC reduced the level of ICAM-1 protein induced by TNF-α possibly through post-translational levels without affecting the TNF-α-induced mRNA expression. However, SAC and SAMC had no effect. It was also confirmed the inhibitory effect of an antimicrobial peptide [human-β defensin-3 (hβD3)] and found that the inhibitory effects of hbD3 and S1PC were synergistic. On the other hand, the TNF-α-induced IL-6 secretion was attenuated by SAC and SAMC in a dose-dependent manner, whereas S1PC was ineffective. In addition, SAC and SAMC, but not S1PC inhibited the phosphorylation of the transcription factor nuclear factor κ-light-chain-enhancer of activated B cells (NF-κB), which is involved in the expression of inflammatory molecules, suggesting that the anti-inflammatory effects of SAC and SAMC are mediated, at least partly, by NF-κB. On the whole, the findings of this study suggest that the three sulfur amino acids in AGE function synergistically in alleviating inflammation in human gingival epithelial cells.

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Aged garlic extract enhances heme oxygenase-1 and glutamate-cysteine ligase modifier subunit expression via the nuclear factor erythroid 2–related factor 2–antioxidant response element signaling pathway in human endothelial cells

Cited by 45 publications

References 47 publications

Pikuni-Blackfeet traditional medicine: Neuroprotective activities of medicinal plants used to treat Parkinson’s disease-related symptoms

Pikuni-Blackfeet traditional medicine: Neuroprotective activities of medicinal plants used to treat Parkinson’s disease-related symptoms

Antihypertensive effect of allicin in dexamethasone-induced hypertensive rats

Sulfur‑containing amino acids in aged garlic extract inhibit inflammation in human gingival epithelial cells by suppressing intercellular adhesion molecule‑1 expression and IL‑6 secretion

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