Abstract:Dengue is the most widespread arbovirus infection and poses a serious health and economic issue in tropical and subtropical countries. Currently no licensed vaccine or compounds can be used to prevent or manage the severity of dengue virus (DENV) infection. Honokiol, a lignan biphenol derived from the Magnolia tree, is commonly used in Eastern medicine. Here we report that honokiol has profound antiviral activity against serotype 2 DENV (DENV-2). In addition to inhibiting the intracellular DENV-2 replicon, hon… Show more
“…Honokiol anticancer activity has been extensively investigated, but the study of honokiol antiviral activity remains limited. Only two reports have shown that DENV-2 and HCV infection are inhibited by honokiol with different underlying mechanisms (Lan et al 2012;Fang et al 2015). In the current study, we show that honokiol inhibits HSV-1 infection through blocking HSV-1 viral DNA replication and gene expression, the combination of honokiol with clinical antiherpesvirus drug acyclovir enhanced inhibition of HSV-1 infection.…”
Section: Discussionmentioning
confidence: 99%
“…Previous studies have also demonstrated the impact of honokiol on microbial infection. Honokiol not only inhibits various human pathogenic fungi infection (Bang et al 2000;Jin et al 2010;Jacobo-Salcedo Mdel et al 2011) and functions as an antibacterial agent (Park et al 2004;Li et al 2016;Sakaue et al 2016), also blocks hepatitis C virus (HCV) infection by targeting cell entry and viral replication (Lan et al 2012) and dengue virus type 2 infection (DENV-2) by inhibiting viral replication, gene expression and endocytotic process of DENV-2 (Fang et al 2015). However, honokiol shows weak activity against HIV-1 in human lymphocytes (Amblard et al 2006).…”
Honokiol is a pleiotropic natural compound isolated from Magnolia and has multiple biological and clinically relevant effects, including anticancer and antimicrobial function. However, the antiviral activity of honokiol has not yet been well studied. Here we showed that honokiol had no effect on herpes simplex virus-1 (HSV-1) entry, but inhibited HSV-1 viral DNA replication, gene expression and the production of new progeny viruses. The combination of honokiol and clinical drug acyclovir augmented inhibition of HSV-1 infection. Our results illustrate that honokiol could be a potential new candidate for clinical consideration in the treatment of HSV-1 infection alone or combination with other therapeutics. Keywords Honokiol Á Herpes simplex virus-1 (HSV-1) Á Viral replication Á Anti-viral activity Á Acyclovir Shuai Liu and Long Li these authors have contributed equally contribution to the work.
“…Honokiol anticancer activity has been extensively investigated, but the study of honokiol antiviral activity remains limited. Only two reports have shown that DENV-2 and HCV infection are inhibited by honokiol with different underlying mechanisms (Lan et al 2012;Fang et al 2015). In the current study, we show that honokiol inhibits HSV-1 infection through blocking HSV-1 viral DNA replication and gene expression, the combination of honokiol with clinical antiherpesvirus drug acyclovir enhanced inhibition of HSV-1 infection.…”
Section: Discussionmentioning
confidence: 99%
“…Previous studies have also demonstrated the impact of honokiol on microbial infection. Honokiol not only inhibits various human pathogenic fungi infection (Bang et al 2000;Jin et al 2010;Jacobo-Salcedo Mdel et al 2011) and functions as an antibacterial agent (Park et al 2004;Li et al 2016;Sakaue et al 2016), also blocks hepatitis C virus (HCV) infection by targeting cell entry and viral replication (Lan et al 2012) and dengue virus type 2 infection (DENV-2) by inhibiting viral replication, gene expression and endocytotic process of DENV-2 (Fang et al 2015). However, honokiol shows weak activity against HIV-1 in human lymphocytes (Amblard et al 2006).…”
Honokiol is a pleiotropic natural compound isolated from Magnolia and has multiple biological and clinically relevant effects, including anticancer and antimicrobial function. However, the antiviral activity of honokiol has not yet been well studied. Here we showed that honokiol had no effect on herpes simplex virus-1 (HSV-1) entry, but inhibited HSV-1 viral DNA replication, gene expression and the production of new progeny viruses. The combination of honokiol and clinical drug acyclovir augmented inhibition of HSV-1 infection. Our results illustrate that honokiol could be a potential new candidate for clinical consideration in the treatment of HSV-1 infection alone or combination with other therapeutics. Keywords Honokiol Á Herpes simplex virus-1 (HSV-1) Á Viral replication Á Anti-viral activity Á Acyclovir Shuai Liu and Long Li these authors have contributed equally contribution to the work.
“…Elsholtzia contains flavonoids and coumarins, and has the functions of heat clearing, wetting, antibacterial, anti-inflammatory and other pharmacological actions [19]. Honokiol in Magnolia officinalis also has the effect of inhibiting virus replication [20,21]. In the present study, XJXRY clearly demonstrated therapeutic effects in mice infected with influenza in a hot and humid environment, and reduced pulmonary inflammation in normal WT mice, but had no significant effect on TLR7 −/− mice.…”
Background
To investigate the effects and immunological mechanisms of the traditional Chinese medicine Xinjiaxiangruyin on controlling influenza virus (FM1 strain) infection in mice housed in a hygrothermal environment.
Methods
Mice were housed in normal and hygrothermal environments, and intranasally infected with influenza virus (FM1). A high-performance liquid chromatography fingerprint of Xinjiaxiangruyin was used to provide an analytical method for quality control. Real-time quantitative polymerase chain reaction (RT-qPCR) was used to measure messenger RNA expression of Toll-like receptor 7 (TLR7), myeloid differentiation primary response 88 (MyD88), and nuclear factor-kappa B (NF-κB) p65 in the TLR7 signaling pathway and virus replication in the lungs. Western blotting was used to measure the expression levels of TLR7, MyD88, and NF-κB p65 proteins. Flow cytometry was used to detect the proportion of Th17/T-regulatory cells.
Results
Xinjiaxiangruyin effectively alleviated lung inflammation in C57BL/6 mice in hot and humid environments. Guizhimahuanggebantang significantly reduced lung inflammation in C57BL/6 mice. The expression of TLR7, MyD88, and NF-κB p65 mRNA in lung tissue of WT mice in the normal environment, GZMHGBT group was significantly lower than that in the model group (P < 0.05). In WT mice exposed to the hot and humid environment, the expression levels of TLR7, MyD88, and NF-κB p65 mRNA in the XJXRY group were significantly different from those in the virus group. The expression levels of TLR7, MyD88, and NF-κB p65 protein in lung tissue of WT mice exposed to the normal environment, GZMHGBT group was significantly lower than those in the model group. In WT mice exposed to hot and humid environments, the expression levels of TLR7, MyD88, and NF-κB p65 protein in XJXRY group were significantly different from those in the virus group.
Conclusion
Guizhimahuanggebantang demonstrated a satisfactory therapeutic effect on mice infected with the influenza A virus (FM1 strain) in a normal environment, and Xinjiaxiangruyin demonstrated a clear therapeutic effect in damp and hot environments and may play a protective role against influenza through downregulation of the TLR7 signal pathway.
“…In addition, magnolol, the main component of Cortex Magnoliae Officinalis, has antioxidative [ 16 , 17 ], inflammation regulation [ 18 ], cancer cell inhibition [ 19 ] and other effects. At the same time, honokiol also has an anti-viral activity [ 20 , 21 ].…”
BackgroundInfluenza virus is a single-stranded RNA virus that causes influenza in humans and animals. About 600 million people around the world suffer from influenza every year. Upon recognizing viral RNA molecules, TLR7 (Toll-like receptor) initiates corresponding immune responses. Traditional Chinese Medicines (TCMs), including Yinqiao powder, Xinjiaxiangruyin and Guizhi-and-Mahuang decoction, have been extensively applied in clinical treatment of influenza. Although the therapeutic efficacy of TCMs against influenza virus in vivo was reported previously, its underlying mechanisms are not clearly understood. This study aimed to investigate the immunological mechanisms in the treatment of influenza virus infected mice with three Chinese herbal compounds as well as the effect on TLR7/NF-κB signaling pathway during recovery.MethodsWild type and TLR7 KO C57BL/6 mice were infected with influenza virus FM1 and then treated with three TCMs. The physical parameters of mice (body weight and lung index) and the expression levels of components in TLR7/NF-κB signaling pathway were evaluated.ResultsAfter viral infection, Guizhi-and-Mahuang decoction and Yinqiao powder showed better anti-viral effect under normal condition. Compared to the viral control group, expression levels of TLR7, MyD88, IRAK4 and NF-κB were significantly reduced in all treatment groups. Furthermore, the three TCM treatment groups showed poor therapeutic efficacy and no difference in viral load compared to the viral control group in TLR7 KO mice.ConclusionOur study indicated that Guizhi-and-Mahuang decoction and Yinqiao powder might play a crucial role of anti-influenza virus by regulating TLR7/NF-κB signal pathway.Electronic supplementary materialThe online version of this article (10.1186/s13020-018-0199-4) contains supplementary material, which is available to authorized users.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.