A Foodborne Outbreak of Gastroenteritis Caused by Vibrio parahaemolyticus and Norovirus through Non-Seafood Vehicle

Liu, Yan‐Hui; Tam, Yat Hung; Yuan, Jun; Chen, Fengling; Cai, Wenfeng; Liu, Jianping; Ma, Xiaowei; Xie, Chunguang; Zheng, Chuangliang; Li, Zhuo; Cao, Xianbang; Tan, Hailing; Li, Baisheng; Xie, Hua; Liu, Yuanyuan; Ip, Dennis K. M.

doi:10.1371/journal.pone.0137848

Cited by 21 publications

(14 citation statements)

References 13 publications

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“…parahaemolyticus was first isolated in 1952, reports demonstrated the recent outbreaks of V . parahaemolyticus are more severe [ 10 , 11 ]. On the recent outbreak in the city of Osaka (Japan), acute gastroenteritis was reported in 272 individuals, 20 of whom died [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Comprehensive genome based analysis of Vibrio parahaemolyticus for identifying novel drug and vaccine molecules: Subtractive proteomics and vaccinomics approach

et al. 2020

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Multidrug-resistant Vibrio parahaemolyticus has become a significant public health concern. The development of effective drugs and vaccines against Vibrio parahaemolyticus is the current research priority. Thus, we aimed to find out effective drug and vaccine targets using a comprehensive genome-based analysis. A total of 4822 proteins were screened from V. parahaemolyticus proteome. Among 16 novel cytoplasmic proteins, 'VIBPA Type II secretion system protein L' and 'VIBPA Putative fimbrial protein Z' were subjected to molecular docking with 350 human metabolites, which revealed that Eliglustat, Simvastatin and Hydroxocobalamin were the top drug molecules considering free binding energy. On the contrary, 'Sensor histidine protein kinase UhpB' and 'Flagellar hook-associated protein of 25 novel membrane proteins were subjected to T-cell and B-cell epitope prediction, antigenicity testing, transmembrane topology screening, allergenicity and toxicity assessment, population coverage analysis and molecular docking analysis to generate the most immunogenic epitopes. Three subunit vaccines were constructed by the combination of highly antigenic epitopes along with suitable adjuvant, PADRE sequence and linkers. The designed vaccine constructs (V1, V2, V3) were analyzed by their physiochemical properties and molecular docking with MHC molecules-results suggested that the V1 is superior. Besides, the binding affinity of human TLR-1/2 heterodimer and construct V1 could be biologically significant in the development of the vaccine repertoire. The vaccine-receptor complex exhibited deformability at a minimum level that also strengthened our prediction. The optimized codons of the designed construct was cloned into pET28a(+) vector of E. coli strain K12. However, the predicted drug molecules and vaccine constructs could be further studied using model animals to combat V. parahaemolyticus associated infections.

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Section: Introductionmentioning

confidence: 99%

Comprehensive genome based analysis of Vibrio parahaemolyticus for identifying novel drug and vaccine molecules: Subtractive proteomics and vaccinomics approach

et al. 2020

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“…Subsequent investigations revealed that one of the food handlers was the actual carrier/source for both pathogens. 62 A larger Vibrio outbreak in Malaysia in November 2009, caused approximately 400 infections and one casualty; several restaurants and food factories were ordered to close as they were suspected to be responsible for the outbreak. 63 In such cases, multiplex PCR assays would allow the fast identification of potential sources and the differentiation of disease-causing biotypes.…”

Section: Resultsmentioning

confidence: 99%

Utilization of Small RNA Genes to Distinguish Vibrio cholerae Biotypes via Multiplex Polymerase Chain Reaction

Ahmed

Raabe

Cheah

et al. 2019

The American Journal of Tropical Medicine and Hygiene

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The diarrheal disease "cholera" is caused by Vibrio cholerae, and is primarily confined to endemic regions, mostly in Africa and Asia. It is punctuated by outbreaks and creates severe challenges to public health. The disease-causing strains are most-often members of serogroups O1 and O139. PCR-based methods allow rapid diagnosis of these pathogens, including the identification of their biotypes. However, this necessitates the selection of specific target sequences to differentiate even the closely related biotypes of V. cholerae. Oligonucleotides for selective amplification of small RNA (sRNA) genes that are specific to these V. cholerae subtypes were designed. The resulting multiplex PCR assay was validated using V. cholerae cultures (i.e., 19 V. cholerae and 22 non-V. cholerae isolates) and spiked stool samples. The validation using V. cholerae cultures and spiked stool suspensions revealed detection limits of 10-100 pg DNA per reaction and 1.5 cells/mL suspension, respectively. The multiplex PCR assay that targets sRNA genes for amplification enables the sensitive and specific detection, as well as the differentiation of V. cholerae-O1 classical, O1 El Tor, and O139 biotypes. Most importantly, the assay enables fast and cheaper diagnosis compared with classic culture-based methods.

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“…), listeriosis (from Listeria sp. ), Clostridium perfringens , and V. parahaemolyticus gastroenteritis have been reported yet, even if they have been reportedly transmitted by the fecal‐oral route (Bari, Hossain, Isshiki, & Ukuku, ; Battersby, Whyte, & Bolton, ; Deleo & Otto, ; Jimenez et al, ; Liu et al, ; Papaconstantinou & Thomas, ; Shetty, ). There have been no reports of fecal‐oral or direct contact transmission of Vibrio sepsis (from V. vulnificus ) (J. M. Park, You, Cho, et al, ), even though confirmed cases could discharge the causative agent via their feces (Bross, Soch, Morales, & Mitchell, ).…”

Section: Resultsmentioning

confidence: 99%

New procedures for food handlers under infectious gastrointestinal disease: To control emerging microbial problems

Park

Cho²,

Kim³

et al. 2018

Journal of Food Safety

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Owing to climate change and global warming, foodborne infectious diseases are becoming an inevitable problem worldwide, and this includes Korea. An emerging factor of foodborne infectious disease outbreak in the foodservice business (i.e., restaurants) is inappropriate management of food handlers with acute gastroenteritis. This study will propose new procedures for bigger restaurants such as conglomerate chains or hotel restaurants that have adequate economic capability for operating new or existing procedures to complement the shortcomings of the Korean Food Safety Act based on microbiological, epidemiological, and etiological fundamentals. Designed procedures for managing a primary case (i.e., first symptomatic worker) or confirmed patients in close contact (i.e., colleagues) were based on several concepts, such as isolation, contact quarantine, and release. These procedures can be included in the sanitation standard operating procedures for foodservice business in a chapter on the Food Handler's Hygiene Management Manual. Practical applications Foodborne infectious disease spread by food handlers is currently an emerging food safety management crisis in Korea. Since few response procedures have been proposed as to how to act in such a critical situation, our study will suggest new procedures. Possible foodborne infectious disease that can be mediated by food handlers was investigated and categorized into four types based on their microbiological, epidemiological, and etiological characters. Several distinct response procedures were designated for primary or secondary case, confirmed case, and close contact colleagues. Newly designed criteria were simulated for field applicability. These procedures can be included in the sanitation standard operating procedures for foodservice businesses in a chapter of the Food Handler's Hygiene Management Manual.

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A Foodborne Outbreak of Gastroenteritis Caused by Vibrio parahaemolyticus and Norovirus through Non-Seafood Vehicle

Cited by 21 publications

References 13 publications

Comprehensive genome based analysis of Vibrio parahaemolyticus for identifying novel drug and vaccine molecules: Subtractive proteomics and vaccinomics approach

Comprehensive genome based analysis of Vibrio parahaemolyticus for identifying novel drug and vaccine molecules: Subtractive proteomics and vaccinomics approach

Utilization of Small RNA Genes to Distinguish Vibrio cholerae Biotypes via Multiplex Polymerase Chain Reaction

New procedures for food handlers under infectious gastrointestinal disease: To control emerging microbial problems

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