Herb-Partitioned Moxibustion Regulates the TLR2/NF-<i>κ</i>B Signaling Pathway in a Rat Model of Ulcerative Colitis

Wang, Xiaomei; Liu, Yanan; Dong, Hongsheng; Wu, Luyi; Feng, Xiaoming; Zhou, Zhigang; Zhao, Chen; Liu, Huirong; Wu, Huangan

doi:10.1155/2015/949065

Cited by 26 publications

(13 citation statements)

References 29 publications

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“…Additionally, a recent review highlighted the role of TNFα and TNFR1 activation in the progression of the inflammatory response and consequent activation of NFκB signaling pathway in different inflammatory diseases including UC (Holbrook et al, 2019). It was also reported in numerous literatures that NFκB pathway plays a central role in regulating the release of cytokines and contributes to the inflammatory and immune response in patients with UC (Sakthivel and Guruvayoorappan, 2014, Wang et al, 2015), which was consistent with our findings where the induction of UC in rats using AA increased the level TNFα, TNFR1 that consequently augmented the activation of NFκB p65.…”

Section: Discussionsupporting

confidence: 93%

Protective effect ofMorus macrouraMiq. fruit extract against acetic acid-induced ulcerative colitis in rats: Involvement of miRNA-223 and TNFα/NFκB/NLRP3 inflammatory pathway

Salama

Darwish

Shaffei

et al. 2020

Preprint

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Tumor necrosis factor receptor (TNFR) activation and nuclear factor kappa B (NFκB) expression play a significant role in the activation of nod-like receptor pyrin domain-1 containing 3 (NLRP3) inflammasome inflammatory pathway, which is involved in the pathogenesis of ulcerative colitis (UC). Furthermore, miRNA-223 expression was shown to exert counter-regulatory effect on NLRP3 expression. Interestingly, polyphenols are attaining increased importance for their potential effectiveness in ameliorating certain diseases owing to their antioxidant and anti-inflammatory activity. In accord, our study attempted to investigate the effect of mulberry tree (Morus macroura) fruit extract (MFE) against acetic acid (AA)- induced UC in rats, which is not previously investigated, based on previous promising results for MFE in alleviating gastric ulcer in rats. First, total phenolic (TPC), total flavonoid (TFC), and total anthocyanin content (TAC) were determined in MFE. Then, MFE (300 mg/kg) and sulfasalazine (Sulfa), as a standard treatment (100 mg/kg), were given orally for seven days before intra-rectal induction of UC by AA (2 ml, 4% v/v) on day eight. The extent of UC was evaluated macroscopically and microscopically. Biochemically, the colonic TNFR1, NLRP3, p- NFκB p65, TNFα, interleukin-1 beta (IL-1β), IL-18 levels, miRNA-223 expression and caspase-1 activity were assayed. MFE significantly reduced macroscopic and microscopic scores, colonic levels of TNFR1, NLRP3, p-NFκB p65, TNFα, IL-1β, IL-18, and caspase-1 activity, and showed increased miRNA-223 expression, almost similarly to Sulfa effects. In conclusion, our study provided a novel impact for MFE against AA-induced UC in rats through affecting miRNA-223 expression and halting TNFα/NFκB/NLRP3 inflammatory pathway.

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Section: Discussionsupporting

confidence: 93%

Protective effect ofMorus macrouraMiq. fruit extract against acetic acid-induced ulcerative colitis in rats: Involvement of miRNA-223 and TNFα/NFκB/NLRP3 inflammatory pathway

Salama

Darwish

Shaffei

et al. 2020

Preprint

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“…Similar results were also observed in an extract of Huanglian (Coptidis rhizome), a widely used herb in traditional Chinese medicine (25). While production of IFN-β and IL-10 was significantly decreased in the colonic mucosa of ulcerative colitis rats, their production was significantly upregulated upon herb-partitioned moxibustion (26). Fractions obtained from the aqueous extract of B. coccineus, a plant commonly found in west and tropical Africa, significantly stimulated IFN-β expression in PBMCs, causing a seven-fold increase of its transcript (27).…”

Section: Discussionsupporting

confidence: 75%

Matrine Inhibits CNS Autoimmunity Through an IFN-β-Dependent Mechanism

Chu

Thomé

et al. 2020

Front. Immunol.

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Matrine (MAT), a quinolizidine alkaloid component derived from the root of Sophora flavescens, suppresses experimental autoimmune encephalomyelitis (EAE), the animal model of multiple sclerosis (MS), by inducing the production of immunomodulatory molecules, e.g., IL-10. In an effort to find the upstream pathway(s) of the mechanism underlying these effects, we have tested certain upregulated immunomodulatory molecules. Among them, we found increased levels of IL-27 and IFN-β, one of the first-line MS therapies. Indeed, while low levels of IFN-β production in sera and type I interferon receptor (IFNAR1) expression in spinal cord of saline-treated control EAE mice were detected, they were significantly increased after MAT treatment. Increased numbers of CD11b + IFN-β + microglia/infiltrating macrophages were observed in the CNS of MAT-treated mice. The key role of IFN-β induction in the suppressive effect of MAT on EAE was further verified by administration of anti-IFN-β neutralizing antibody, which largely reversed the therapeutic effect of MAT. Further, we found that, while MAT treatment induced production of IL-27 and IL-10 by CNS microglia/macrophages, this effect was significantly reduced by IFN-β neutralizing antibody. Finally, the role of IFN-β in MAT-induced IL-27 and IL-10 production was further confirmed in human monocytes in vitro. Together, our study demonstrates that MAT exerts its therapeutic effect in EAE through an IFN-β/IL-27/IL-10 pathway, and is likely a novel, safe, low-cost, and effective therapy as an alternative to exogenous IFN-β for MS.

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“…The rat model of UC was established using immunization plus topical stimulation with formalin[ 25 , 26 ]. After modeling, each group contributed one rat for histopathological observation to verify the success of the UC model.…”

Section: Methodsmentioning

confidence: 99%

Herb-partitioned moxibustion alleviates colon injuries in ulcerative colitis rats

Zhang

Ren

Wei

et al. 2018

WJG

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AIMTo observe the effect of herb-partitioned moxibustion (HPM) on expression of colonic cytokines in ulcerative colitis (UC) rats.METHODSA UC rat model was established by protein immunization in combination with topical chemical stimulation. Rats in the HPM group (n = 8) received HPM at bilateral Tianshu (ST25) points. The gross injury and pathological scores of the colon were recorded. The expression profile of colonic cytokines was assayed using the protein microarray technique. Specific differential cytokines were selected and verified by ELISA. The corresponding UniProt Accessions of the differentially expressed cytokines were retrieved in the UniProt database. The pathways involved were analyzed with the help of the KEGG PATHWAY database. The DAVID database was used for functional cluster and pathway analysis.RESULTSHPM improved colon injuries in UC rats, manifested by accelerated repair of ulcers and alleviation of inflammation, and the gross injury and pathological scores both significantly decreased (P < 0.01). Fold change > 1.3 or < 0.77 was taken as the screening standard. There were 77 down-regulated and 9 up-regulated differentially expressed colonic cytokines in the HPM group compared with the model group, and expression of 20 differed significantly (P < 0.05). Twelve of the 20 significantly differentially expressed cytokines [β-catenin, interleukin-1 receptor 6 (IL-1R6), IL-1β, B7-1, nerve growth factor receptor, AMP-activated protein kinase-α1, neuropilin-2, orexin A, adipocyte differentiation-related protein, IL-2, Fas and FasL] were up-regulated in the model group (n = 3, compared with the normal group) but down-regulated in the HPM group (n = 3, compared with the model group). Functional cluster analysis showed that the differentially expressed colonic cytokines in the HPM group regulated apoptosis and protein phosphorylation. KEGG pathway analysis showed that 52 down-regulated and 7 up-regulated differentially expressed colonic cytokines in the HPM group had pathways. The pathways that interacted between the cytokines and their receptors accounted for the largest proportion (28 of the down-regulated and 5 of the up-regulated cytokines).CONCLUSIONHPM promotes the repair of colon injuries in UC rats, which is related to the regulation of several abnormally expressed cytokines.

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Herb-Partitioned Moxibustion Regulates the TLR2/NF-κB Signaling Pathway in a Rat Model of Ulcerative Colitis

Cited by 26 publications

References 29 publications

Protective effect ofMorus macrouraMiq. fruit extract against acetic acid-induced ulcerative colitis in rats: Involvement of miRNA-223 and TNFα/NFκB/NLRP3 inflammatory pathway

Protective effect ofMorus macrouraMiq. fruit extract against acetic acid-induced ulcerative colitis in rats: Involvement of miRNA-223 and TNFα/NFκB/NLRP3 inflammatory pathway

Matrine Inhibits CNS Autoimmunity Through an IFN-β-Dependent Mechanism

Herb-partitioned moxibustion alleviates colon injuries in ulcerative colitis rats

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