Acute exposure to waterborne psychoactive drugs attract zebrafish

Abreu, Murilo S. de; Giacomini, Ana C.V.V.; Gusso, Darlan; Rosa, João Gabriel Santos da; Koakoski, Gessi; Kalichak, Fabiana; Idalêncio, Renan; Oliveira, Thiago Acosta; Barcellos, H. H. A.; Bonan, Carla Denise; Barcellos, Leonardo José Gil

doi:10.1016/j.cbpc.2015.08.009

Cited by 32 publications

(28 citation statements)

References 48 publications

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“…Also, its brain structure [24,25] and neurochemistry [26] offer translational relevance to humans [23] and allow exploring the model for a thorough understanding of the effects of substances used/abused by humans. In fact, various studies have shown that zebrafish respond similar to mammals when treated with many pharmacological compounds [27][28][29]. For example, benzodiazepine medication causes sedative effects in mouse [30,31] and zebrafish [32].…”

Section: Introductionmentioning

confidence: 99%

Caffeine Dose-Response Relationship and Behavioral Screening in Zebrafish

Santos¹,

Ruiz-Oliveira²,

Silva³

et al. 2017

The Question of Caffeine

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It has been centuries since humans consume coffee and get the benefits of this bean. Many researches worldwide continue to show healthful properties of coffee, while others suggest a number of side effects. In fact, anything consumed in excess may cause disturbance of the body functioning, whereas caffeine is a central nervous system stimulant that increases focus and improves performance, its high concentration can cause insomnia, dizziness, and vomiting. Thus, the question is: which coffee dose promotes benefits and prevents risks? To answer it, we used the zebrafish, a popular animal model that is at the vanguard of psychopharmacological research due to its unique combination of complexity and simplicity, translational relevance and applicability to high throughput behavioral drug screens. In the current study, we examine time-course and dose-dependent changes in zebrafish following exposure to caffeine. Our data show an inverted U-shaped path for the locomotor parameters and crescent path for the anxiety-like parameters. High doses are harmful to the individual, because the stimulating effect disappears and anxiogenic effects take place. We conclude that temporal analysis of zebrafish behavior is a sensitive method for the study of acute caffeine exposure-induced functional changes in the vertebrate brain.

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Section: Introductionmentioning

confidence: 99%

Caffeine Dose-Response Relationship and Behavioral Screening in Zebrafish

Santos¹,

Ruiz-Oliveira²,

Silva³

et al. 2017

The Question of Caffeine

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“…Stimulation of the ATN elicits reproduction-related vocalizations in male midshipman fishPorichthys notatus(Goodson and Bass, 2000), suggesting a role in reproductive behavior; however, a role in defensive behavior has not yet been determined.Indirect evidence for a participation of the hypothalamus in defensive behavior is stronger in relation to neuroendocrine endpoints, especially cortisol responses, given that these responses are under descending hypothalamic control. Aversive stimuli which have been shown to elicit cortisol responses in zebrafish include acute chasing stress (deAbreu et al, 2016;Idalencio et al, 2017;Tran et al, 2014), acute restraint stress(Abreu et al, 2017a;Ghisleni et al, 2012), unpredictable chronic stress(Piato et al, 2011), exposure to the novel tank or the light/dark test…”

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confidence: 99%

The Aversive Brain System of Teleosts: Implications for Neuroscience and Biological Psychiatry

Silva¹,

Lima-Maximino²,

Maximino³

2018

Preprint

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Defensive behavior is a function of specific survival circuits, the “aversive brain system”, that are thought to be conserved across vertebrates, and involve threat detection and the organization of defensive responses to reduce or eliminate threat. In mammals, these circuits involve amygdalar and hypothalamic subnuclei and midbrain circuits. The increased interest in teleost fishes as model organisms in neuroscience created a demand to understand which brain circuits are involved in defensive behavior. Telencephalic and habenular circuits represent a “forebrain circuit” for threat processing and organization of responses, being important to  mounting appropriate coping responses. Specific hypothalamic circuits organize neuroendocrine and neurovegetative outputs, but are the less well-studied in fish. A “midbrain circuit” is represented by projections to interneurons in the optic tectum which mediate fast escape responses via projections to the central gray and/or the brainstem escape network. Threatening stimuli (especially visual stimuli) can bypass the “high road” and directly activate this system, initiating escape responses. Increased attention to these circuits in an evolutionary framework is still needed.

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“…The experimental apparatus consisted of a 30-L acrylic tank ( Fig. 1a; 50 9 25 9 25 cm, length 9 width 9 height) as described in Abreu et al (2016). Briefly, the apparatus had two chambers leading to two lanes of water with laminar flow running in parallel without mixing.…”

Section: Experimental Apparatusmentioning

confidence: 99%

“…The basic strategy of the present study consisted of a twochoice test first used by Korver and Sprague (1989), then adapted to test anesthetics (Readman et al 2013) and psychoactive drugs (Abreu et al 2016), in which we quantified, in individually reared zebrafish, the choice for paths having contaminated or clean water flows. Choice was operationally set as the time spent in each path and the shuttle frequency between them.…”

Section: Experimental Protocolmentioning

confidence: 99%

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Fish Aversion and Attraction to Selected Agrichemicals

Rosa

Abreu

Giacomini

et al. 2016

Arch Environ Contam Toxicol

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In agriculture intensive areas, fishponds and natural water bodies located in close proximity to these fields receive water with variable amounts of agrichemicals. Consequently, toxic compounds reach nontarget organisms. For instance, aquatic organisms can be exposed to tebuconazole-based fungicides (TBF), glyphosate-based herbicides (GBH), and atrazine-based herbicides (ABH) that are potentially dangerous, which motivates the following question: Are these agrichemicals attractant or aversive to fish? To answer this question, adult zebrafish were tested in a chamber that allows fish to escape from or seek a lane of contaminated water. This attraction and aversion paradigm was evaluated with zebrafish in the presence of an acute contamination with these compounds. We showed that only GBH was aversive to fish, whereas ABH and TBF caused neither attraction nor aversion for zebrafish. Thus, these chemicals do not impose an extra toxic risk by being an attractant for fish, although TBF and ABH can be more deleterious, because they induce no aversive response. Because the uptake and bioaccumulation of chemicals in fish seems to be time- and dose-dependent, a fish that remains longer in the presence of these substances tends to absorb higher concentrations than one that escapes from contaminated sites.

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Acute exposure to waterborne psychoactive drugs attract zebrafish

Cited by 32 publications

References 48 publications

Caffeine Dose-Response Relationship and Behavioral Screening in Zebrafish

Caffeine Dose-Response Relationship and Behavioral Screening in Zebrafish

The Aversive Brain System of Teleosts: Implications for Neuroscience and Biological Psychiatry

Fish Aversion and Attraction to Selected Agrichemicals

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