Proteoliposomes with the ability to transport Ca2+ into the vesicles and hydrolyze phosphosubstrates on their surface

Bolean, Maytê; Simão, Adenilso da Silva; Kiffer-Moreira, Tina; Hoylaerts, Marc; Millán, José Luis; Itri, Rosângela; Ciancaglini, Pietro

doi:10.1016/j.abb.2015.08.018

Cited by 29 publications

(53 citation statements)

References 72 publications

(149 reference statements)

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“…The first step is the deposition of an amorphous mineral complex, i.e. the nucleation core (NC), which crystalizes to form HA inside MVs [5–8]. Concomitantly, MVs bleb out from cell membranes, bind collagen fibrils through specific molecular interactions and decompose to release their HA crystals, enabling HA propagation onto the collagenous ECM.…”

Section: Introductionmentioning

confidence: 99%

Topographic analysis by atomic force microscopy of proteoliposomes matrix vesicle mimetics harboring TNAP and AnxA5

Bolean

Borin

Simão

et al. 2017

Biochimica et Biophysica Acta (BBA) - Biomembranes

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Atomic force microscopy (AFM) is one of the most commonly used scanning probe microscopy techniques for nanoscale imaging and characterization of lipid-based particles. However, obtaining images of such particles using AFM is still a challenge. The present study extends the capabilities of AFM to the characterization of proteoliposomes, a special class of liposomes composed of lipids and proteins, mimicking matrix vesicles (MVs) involved in the biomineralization process. To this end, proteoliposomes were synthesized, composed of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-dipalmitoyl-sn-glycero-3-phospho-l-serine (DPPS), with inserted tissue-nonspecific alkaline phosphatase (TNAP) and/or annexin V (AnxA5), both characteristic proteins of osteoblast-derived MVs. We then aimed to study how TNAP and AnxA5 insertion affects the proteoliposomes’ membrane properties and, in turn, interactions with type II collagen, thus mimicking early MV activity during biomineralization. AFM images of these proteoliposomes, acquired in dynamic mode, revealed the presence of surface protrusions with distinct viscoelasticity, thus suggesting that the presence of the proteins induced local changes in membrane fluidity. Surface protrusions were measurable in TNAP-proteoliposomes but barely detectable in AnxA5-proteoliposomes. More complex surface structures were observed for proteoliposomes harboring both TNAP and AnxA5 concomitantly, resulting in a lower affinity for type II collagen fibers compared to proteoliposomes harboring AnxA5 alone. The present study achieved the topographic analysis of lipid vesicles by direct visualization of structural changes, resulting from protein incorporation, without the need for fluorescent probes.

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Section: Introductionmentioning

confidence: 99%

Topographic analysis by atomic force microscopy of proteoliposomes matrix vesicle mimetics harboring TNAP and AnxA5

Bolean

Borin

Simão

et al. 2017

Biochimica et Biophysica Acta (BBA) - Biomembranes

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“…Biophysical and biochemical characterization of biomimetic models Biophysical tools applied to biomineralization studies A large number of studies have been carried out using proteoliposomes as chondrocyte-and osteoblast-derived MVs biomimetics (Andrade et al 2016;Bolean et al 2010Bolean et al , 2011Bolean et al , 2015Bolean et al , 2017Ciancaglini et al 2010;Favarin et al 2017;Simao et al 2010aSimao et al , b, 2013.…”

Section: What Are the Biomimetic Systems Of Mvs?mentioning

confidence: 99%

Biophysical aspects of biomineralization

et al. 2017

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During the process of endochondral bone formation, chondrocytes and osteoblasts mineralize their extracellular matrix (ECM) by promoting the synthesis of hydroxyapatite (HA) seed crystals in the sheltered interior of membranelimited matrix vesicles (MVs). Several lipid and proteins present in the membrane of the MVs mediate the interactions of MVs with the ECM and regulate the initial mineral deposition and posterior propagation. Among the proteins of MV membranes, ion transporters control the availability of phosphate and calcium needed for initial HA deposition. Phosphatases (orphan phosphatase 1, ectonucleotide pyrophosphatase/ phosphodiesterase 1 and tissue-nonspecific alkaline phosphatase) play a crucial role in controlling the inorganic pyrophosphate/inorganic phosphate ratio that allows MVmediated initiation of mineralization. The lipidic microenvironment can help in the nucleation process of first crystals and also plays a crucial physiological role in the function of MVassociated enzymes and transporters (type III sodiumdependent phosphate transporters, annexins and Na + /K + ATPase). The whole process is mediated and regulated by the action of several molecules and steps, which make the process complex and highly regulated. Liposomes and proteoliposomes, as models of biological membranes, facilitate the understanding of lipid-protein interactions with emphasis on the properties of physicochemical and biochemical processes. In this review, we discuss the use of proteoliposomes as multiple protein carrier systems intended to mimic the various functions of MVs during the initiation and propagation of mineral growth in the course of biomineralization. We focus on studies applying biophysical tools to characterize the biomimetic models in order to gain an understanding of the importance of lipid-protein and lipid-lipid interfaces throughout the process.Keywords Lipid microenvironment . Biomineralization . Matrix vesicles . Liposome . Proteoliposome . Hydroxyapatite Mineralization process and the biogenesis of matrix vesiclesMineralization of cartilage and bone occurs by a series of physicochemical and biochemical processes that together facilitate the deposition of calcium phosphate. The deposited calcium phosphate is subsequently converted into hydroxyapatite (HA) [Ca 10 (PO 4 ) 6 (OH) 2 ] in specific areas of the extracellular matrix (ECM). Various experiments have revealed the presence of HA crystals both inside and outside collagen fibrils in the ECM (McNally et al. 2012) and also within the

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“…Proteoliposomes can be used to study how the protein-lipid interactions specifically affect the surface of the membrane model. The scientific community has highlighted this approach as an excellent tool to understand biochemical and biophysical phenomena and to evaluate biotechnological applications (Camolezi et al 2002;Ierardi et al 2002;Daghastanli et al 2004;De Lima Santos et al 2005;Rigos et al 2008;Santos et al 2009;Bolean et al 2010Bolean et al , 2011Bolean et al , 2015Simão et al 2010aSimão et al , 2010bBarbosa et al 2011;Yoneda et al 2013Yoneda et al , 2014Simão et al 2015;Dong et al 2016;Elkhodiry et al 2016). …”

Section: Liposomes and Nanobiotechnologymentioning

confidence: 99%

Biomedical applications of nanotechnology

et al. 2017

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The ability to investigate substances at the molecular level has boosted the search for materials with outstanding properties for use in medicine. The application of these novel materials has generated the new research field of nanobiotechnology, which plays a central role in disease diagnosis, drug design and delivery, and implants. In this review, we provide an overview of the use of metallic and metal oxide nanoparticles, carbon-nanotubes, liposomes, and nanopatterned flat surfaces for specific biomedical applications. The chemical and physical properties of the surface of these materials allow their use in diagnosis, biosensing and bioimaging devices, drug delivery systems, and bone substitute implants. The toxicology of these particles is also discussed in the light of a new field referred to as nanotoxicology that studies the surface effects emerging from nanostructured materials.

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Proteoliposomes with the ability to transport Ca2+ into the vesicles and hydrolyze phosphosubstrates on their surface

Cited by 29 publications

References 72 publications

Topographic analysis by atomic force microscopy of proteoliposomes matrix vesicle mimetics harboring TNAP and AnxA5

Topographic analysis by atomic force microscopy of proteoliposomes matrix vesicle mimetics harboring TNAP and AnxA5

Biophysical aspects of biomineralization

Biomedical applications of nanotechnology

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